Study Guide for Campbell Biology
11th Edition
ISBN: 9780134443775
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece, Martha R. Taylor, Michael A. Pollock
Publisher: PEARSON
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Chapter 20, Problem 10TYK
Summary Introduction
Introduction: Recombinant deoxyribonucleic acid (DNA) technology is the process of combining the DNA sequence of two or more organisms artificially to acquire the desired protein product. The process is brought about by inserting the gene of interest into a plasmid called recombinant plasmid. This recombinant plasmid is then introduced into a bacterium to produce a clone of cells with the gene of interest.
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Which gene is incorporated into plasmids to detect recombinantcells?a. restriction endonuclease
b. virus receptors
c. a gene for antibiotic resistance
d. reverse transcriptase
A mutation in which of these proteins will lead to increased mutations in all daughter cells?
A. helicase
B. topoisomerase
C. ligase
D. DNA polymerase
What is used to cut the DNA in panels 1 and 2?
a.
Restriction enzymes
b.
DNA polymerases
c.
DNA ligases
d.
RNA polymerases
e.
Reverse transcriptase
Chapter 20 Solutions
Study Guide for Campbell Biology
Ch. 20 - In what ways would third-generation sequencing be...Ch. 20 - The following schematic diagram depicts an...Ch. 20 - Which of the following DNA sequences would most...Ch. 20 - a. When PCR is used to prepare a DNA fragment for...Ch. 20 - a. What are some of the benefits of determining...Ch. 20 - Prob. 6IQCh. 20 - What are some of the practical and ethical...Ch. 20 - Prob. 8IQCh. 20 - Prob. 1SYKCh. 20 - Fill in the table on the previous page on the...
Ch. 20 - Prob. 3SYKCh. 20 - Prob. 1TYKCh. 20 - Prob. 2TYKCh. 20 - Gel electrophoresis is a means of separating...Ch. 20 - Prob. 4TYKCh. 20 - Prob. 5TYKCh. 20 - The following segment of DNA has restriction sites...Ch. 20 - Prob. 7TYKCh. 20 - Prob. 8TYKCh. 20 - Prob. 9TYKCh. 20 - Prob. 10TYKCh. 20 - Prob. 11TYKCh. 20 - Which enzyme is used in the polymerase chain...Ch. 20 - Prob. 13TYKCh. 20 - STRs (short tandem repeats) are a valuable tool...Ch. 20 - Prob. 15TYKCh. 20 - Which of the following has the greatest potential...Ch. 20 - Prob. 17TYKCh. 20 - Petroleum-lysing bacteria are being engineered for...Ch. 20 - Prob. 19TYKCh. 20 - Prob. 20TYK
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- DNA polymerases that corrects errors and mismatches in DNA is called? A. Post replication repair B. Base excision repair C. Nucleotide excision repair D. Proofreadingarrow_forwardChoose the combination of answers that most accurately completes the statement. Which gene is incorporated into plasmids to detect recombinant cells? a. restriction endonuclease c. a gene for antibiotic resistance b. virus receptors d. reverse transcriptasearrow_forwardWhat repair system will have an issue functioning properly if DNA glycosylase loses its function? A. Photoreactivation repair B. Base excision repair C. Nucleotide excision repair D. SOS repairarrow_forward
- Which of these is a polymer made of nucleotides? a. RNA polymerase b. CAS9 c. restriction enzyme d. mRNAarrow_forwardWhich protein directs the recombination exchange with a DNA that has failed a complete DNA replication? A. UvrA B. RecB C. MutC D. RecAarrow_forwardWhich enzyme adds RNA nucleotides to mark the starting point for the synthesis of a new DNA strand? A. DNA polymerase I B. DNA polymerase III C. primase D. helicase The spontaneous loss of amino groups from adenine in dna results in hypoxanthine an uncommon base opposite thymine. what combination of proteins could repair such damage? A. telomerase, primase, DNA polymerase B. nuclease, DNA polymerase, DNA ligase C. telomerase, helicase, single-strand binding protein D. DNA ligase, replication fork proteins, adenylyl cyclase A section of DNA has the following sequence of nitrogenous bases: CGATTACAG. Which of the following sequence would be produced as a result of transcription? A. CGTUUTCTG B. GCTAATGTC C. CGAUUACAG D. GUAAUGUCarrow_forward
- Which type of mechanism best repairs thymidine dimers caused by exposure to UV light? a. Nucleotide excision repair b. Base excision repair c. Mismatch repair d. NHEJarrow_forwardA scientist randomly mutates the DNA of a bacterium. She then sequences the bacterium’s daughter cells, and finds that the daughters have many errors in their replicated DNA. The parent bacterium likely acquired a mutation in which enzyme? a. DNA ligase b. DNA pol II c. Primase d. DNA pol Iarrow_forwardWhat is the purpose of the low temperature step in the PCR reaction? a. To allow DNA polymerase to synthesize new DNA in the 3' to 5' direction b. To permanently deactivate DNA polymerase c. To allow primers to anneal to DNA templates d. To allow DNA polymerase to synthesize new DNA in the 5' to 3' directionarrow_forward
- What would be the result if an organism’s telomerase were mutated and nonfunctional? a. No DNA replication would take place. b. The DNA polymerase enzyme would stall at the telomere. c. Chromosomes would shorten with each new generation. d. RNA primers could not be removed.arrow_forwardWhat is the effect of ionizing radiation on DNA? a. faster replication b. the formation of nucleotide dimers c. kinks in the DNA molecule d. breaks in the DNA molecule e. base pair mismatchingarrow_forwardWhat is meant by the term DNA replication? a. synthesis of nucleotides b. cell division c. interpretation of the genetic code d. the exact copying of the DNA code into two new moleculesarrow_forward
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