Discuss the process and give example on the following. a. Aptamer Biosensors b. CRISPR c. Stem Cell Therapy

Q: Explain how the presence of an antibiotic resistance gene can be used to determine whether a…

A: Desired genes can be inserted into the genome of an organism using plasmids. In addition to the gene…

Q: Electrophoretic-Mobility Shift Assay DNA affinity chromatography Immunoprecipitation DNA footprint…

A: Introduction  DNA replication is the process by which a cell makes a copy of its genetic material,…

Q: Define the following terms:a. hypochromic effectb. DNA denaturationc. restriction endonucleasesd.…

A: Recombinant DNA is made by combining two or more DNA from other sources. The process depends on the…

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Q: What would be some major impediments to genetically modifyinghuman embryos with CRISPR?

A: Genome editing tools are those tools which help in making changes into the genome of an individual.…

Q: Which of the following statements about base editing is correct? 1. It involves nCas9. 2. The end…

A: Base editing  It is an alternative tool to HDR-mediated replacement. This facilitates editing of…

Q: Describe the methods of site-directed mutagenesis and CRISPR-Cas technology.

A: A molecular process in which some discontinuous change to nucleotide sequences in their genomic DNA…

Q: Order the following experimental steps to identify auxotrophic mutants in Saccharomyces Cerevisiae…

A: The objective of the question is to order the steps to identify auxotrophic mutants in Saccharomyces…

Q: When treating a person by gene therapy, is it necessary that the therapeutic gene becomes part of a…

A: Gene therapy refers to the transfer of nucleic acids into a cell of a patient as a drug for the…

Q: In gene cloning, a vector is required to transform the gene of interest into host cell. State the…

A: In gene cloning, a vector is required to transform the gene of interest into the host cell. The…

Q: Determine how screening is carried out if pCR®II-TOPO® is used for cloning?

A: There are many applications of PCR as used in DNA fingerprinting to identify an individual from…

Q: Match the term with its description.

A: There are various enzymes and gene components that are studied in techniques of genetic engineering.…

Q: Make a list of possible research questions that could be answeredusing site-directed mutagenesis or…

A: Site-directed mutagenesis refers to an in-vitro or artificial technique with the help of which…

Q: Link the technique to the correct enzyme.…

A:

Q: Describe what CRISPR is and explain the overall process of how it works. Identify and discuss 3-4…

A: CRISPR : It stands for Clustered Regularly Interspaced Short Palindromic Repeats  Repetitive DNA…

Q: What is the benefit of using a strain of Lactobacillus bacterium that contains the CRISPR locus in…

A: CRISPR is a group of DNA sequences present in the genomes of archaea and bacteria, which are…

Q: The detection of single nucleotide polymorphisms is possible with: O A. Restriction Enzymes O B. All…

A: SNPs or single nucleotide polymorphisms are the most common type of genetic variation in humans.…

Q: The student sets up the assay and analyzes the presence of the oncogene in normal and tumor issue…

A: •Cancer cells have more genetic changes than normal cells. •Genetic changes that lead to cancer…

Q: A graduate student who has not taken BIOL 519 thinks they have discovered a new oncogene that could…

A: Cancer develops as a result of damaged genes. These damaged genes are frequently caused by…

Q: Briefly outline how a mutagenesis screen is carried out.

A: Mutagenesis screening can be defined as an experimental technique that helps to screen out those…

Q: Discuss the process and give example on the following: a. Genome Project-write b. Mitochondrial…

A: The process of genome writing, mitochondrial DNA "spring cleaning," micro-encapsulation, and…

Q: Positional cloning refers to: a. using a selection procedure to clone a cDNA b. cloning a portion of…

A: Positional cloning is a gene cloning method where cloning is done simply based on the knowledge of…

Q: The CRISPR method is used in  a.DNA sequencing. b.DNA fingerprinting. c.genome editing. d.the…

A: CRISPER stands for clustered regularly interspaced short palindromic repeats. It is a group of DNA…

Q: What is the function of the CRISPR/Cas system? What are the ethical impacts of a technology such as…

A: Genetic and molecular biology is the study of genetic information such as the genetic material,…

Q: Which of the following is NOT a true statement about CRISPR/Cas9? A. CRISPR/Cas9 is simpler to…

A: E. Delivery of CRISPR/Cas9 is not at all easy.

Q: Outline the steps by which the polymerase chainreaction (PCR) amplifies a specific region of a…

A: Polymerase chain reaction or PCR technique is used to amplify or make multiple copies of a specific…

Q: Explain The Discovery of CRISPR ?

A: The common technology   that can be used to edit genes is refers as the CRISPR. In other words you…

Q: These functional groups act as acid/ base catalysts: A. His imidazole B. Thiol of Cys C. Guanidino…

A: Acid base catalysis is most common type of catalysis mechanism utilized by most of the enzymes like…

Q: RNA microarrays can be used to determine which genes are turned on Select one: a. False b. True

A: The gene expression is the production of RNA from a particular DNA (gene) by the process of…

Q: You have a patient slated to undergo somatic cell gene therapy to alleviate a cystic fibrosis…

A: Answer: Cystic fibrosis is the result of homozygous recessive mutations in the CFTR gene.

Q: Define the following terms: a. ANT b. symporter c. phosphate translocase d. CK e. CK/PCr shuttle

A: Symporter: It is a integral membrane protein that involves in the transport of two different…

Q: Restriction maps illustrate the lengths of DNA fragments between restriction sites. Which of the…

A: Restriction mapping is a technique to map unknown segment of DNA by breakung it into small…

Q: Genetic information from the Human Genome Project may be used to develop screening procedures which…

A: The Human Genome Project was an international, collaborative research project whose aim was to map…

Q: Design the main steps of cloning experiment.

A: Microbial enzymes have been employed in a wide range of sectors, including chemical, agricultural,…

Q: Which of the following methods use(s) a labeled nucleic acidprobe, such as a labeled fragment of…

A: DNA is the genetic material that carries genetic information in the form of coded nucleotide…

Q: Describe CRISPR as a genetic tool with its functions .

A: CRISPR ( Clustered Regularly Interspersed Short Palindromic Repeats ) is a segment of prokaryotic…

Q: Explain why an investigator might want to create aconditional knockout mouse.

A: A conditional Knockout mouse defines an animal model in which a gene of interest can be: inactivated…

Q: Briefly describe what CRISPR technology does

A: Gene is a particular segment of deoxyribose nucleic acid (DNA) that is responsible for the…

Q: which of the following do researchers not need to use during vector cloning? a. a plasmid containing…

A: Certainly! Let's delve deeper into each option:a. Plasmid containing selectable marker genes: When…