Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 19.5, Problem 5COMQ
An advantage of translesion-replicating polymerases is that they can replicate _____________, but a disadvantage is that they _____________.
a. very quickly, have low fidelity
b. over damaged DNA, have low fidelity
c. when
d. over damaged DNA, are very slow
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Students have asked these similar questions
What would be the outcome if the primers used in a polymerase chain reaction have lower GC content (<40 %), shorter, and more variable than the intended oligonucleotide sequence?
a.The PCR reaction will cease after the first cycle
b.The reaction will yield a mixture of non-specific products.
c.All of these
d.The PCR reaction will not start.
e.The reaction will yield a single short PCR product.
Polymerase Chain Reaction, or PCR, can
Group of answer choices
A. target a specific region of DNA and cut it out of the rest of the genetic material for further analysis.
B. copy the number of copies of a selected region of DNA linearly.
C. increase the number of copies of a selected region of DNA exponentially.
D. copy the entire genome at least a dozen times.
Match the terms associated with the polymerase chain reaction with their correct descriptions.
Refers to the fact that DNA molecules get longer the more of them there are in the reaction.
A.
В.
Heat the sample to a high temperature (usually 94°C) to separate all DNA strands from each other.
Denaturation
C.
Incubate the reaction at the optimal temperature for the primers to base-pair with each other.
Annealing.
D.
Incubate at a low enough temperature (usually-55°C) so that primers base-pair with their complementary sequence.
Extension.
Add a chaotropic agent that destabilizes hydrogen bonding.
E.
Amplification.
F. Incubate the sample at a temperature that is optimal for thermostable Taq DNA polymerase (usually -72°C).
G.
Happens after repeated cycles of the temperature change regimen.
Refers to the quadrupling of the target DNA sequence in every cycle of the temperature regimen.
Н.
Chapter 19 Solutions
Genetics: Analysis and Principles
Ch. 19.1 - 1. A mutation changes a codon that specifies...Ch. 19.1 - A down promoter mutation causes the promoter of a...Ch. 19.1 - 3. A mutation in one gene that reverses the...Ch. 19.1 - Which of the following is an example of a somatic...Ch. 19.2 - Prob. 1COMQCh. 19.3 - Which of the following is not an example of a...Ch. 19.3 - A point mutation could be caused by a....Ch. 19.3 - One way that TNRE may occur involves the formation...Ch. 19.4 - Nitrous acid replaces amino groups with keto...Ch. 19.4 - Prob. 2COMQ
Ch. 19.4 - Prob. 3COMQCh. 19.5 - The function of photolyase is to repair a....Ch. 19.5 - Which of the following DNA repair systems may...Ch. 19.5 - 3. In nucleotide excision repair in E. coli, the...Ch. 19.5 - Prob. 4COMQCh. 19.5 - An advantage of translesion-replicating...Ch. 19 - Is each of the following mutations a transition,...Ch. 19 - Prob. 2CONQCh. 19 - What does a suppressor mutation suppress? What is...Ch. 19 - Prob. 4CONQCh. 19 - X-rays strike a chromosome in a living cell and...Ch. 19 - Prob. 6CONQCh. 19 - Prob. 7CONQCh. 19 - 8. A point mutation occurs in the middle of the...Ch. 19 - Prob. 9CONQCh. 19 - Prob. 10CONQCh. 19 - 11. Is a random mutation more likely to be...Ch. 19 - 12. Which of the following mutations could be...Ch. 19 - Prob. 13CONQCh. 19 - Discuss the consequences of a germ-line versus a...Ch. 19 - Prob. 15CONQCh. 19 - Explain how a mutagen can interfere with DNA...Ch. 19 - What type of mutation (transition, transversion,...Ch. 19 - Explain what happens to the sequence of DNA during...Ch. 19 - Distinguish between spontaneous and induced...Ch. 19 - Prob. 20CONQCh. 19 - Prob. 21CONQCh. 19 - Prob. 22CONQCh. 19 - Trinucleotide repeat expansions (TNREs) are...Ch. 19 - 24. With regard to TNRE, what is meant by the term...Ch. 19 - 25. What is the difference between the mutation...Ch. 19 - Achondroplasia is a rare form of dwarfism. It is...Ch. 19 - Prob. 27CONQCh. 19 - In the treatment of cancer, the basis for many...Ch. 19 - Prob. 29CONQCh. 19 - 30. Which of the following examples is likely to...Ch. 19 - Prob. 31CONQCh. 19 - Prob. 32CONQCh. 19 - Prob. 33CONQCh. 19 - With regard to the repair of double-strand breaks,...Ch. 19 - Prob. 35CONQCh. 19 - Prob. 36CONQCh. 19 - 37. Three common ways to repair changes in DNA...Ch. 19 - Prob. 38CONQCh. 19 - Prob. 39CONQCh. 19 - Explain how the technique of replica plating...Ch. 19 - 2. Outline how you would use the technique of...Ch. 19 - 3. From an experimental point of view, is it...Ch. 19 - Prob. 4EQCh. 19 - Prob. 5EQCh. 19 - 6. Richard Boyce and Paul Howard-Flanders...Ch. 19 - In E. coli, a variety of mutator strains have been...Ch. 19 - 2. Discuss the times in a person’s life when it is...Ch. 19 - A large amount of research is aimed at studying...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Which of the following describes an advantage of using a recombinant plasmid for DNA cloning over PCR? A. PCR is more likely to have errors introduced in the copying process. B. Recombinant DNA plasmids are able to create large amounts of copies more quickly than PCR. C. PCR can only be conducted in eukaryotic cells. D. PCR requires prior knowledge of the sequence in question, while a recombinant plasmid does not.arrow_forwardWhich of the following is not essential to carry out the polymerasechain reaction?a. primers b. DNA polymerase c. gel electrophoresis d. high temperaturearrow_forwardWhy is Taq polymerase used in PCR rather than other DNA polymerases?a. Taq polymerase is a synthetic enzyme that produces DNA strands ata faster rate than natural polymerases.b. Taq polymerase is a heat-stable form of DNA polymerase that canfunction after exposure to the high temperatures necessary for PCR.c. Taq polymerase is easier to isolate than other DNA polymerases.d. Taq polymerase is the DNA polymerase commonly produced bymost eukaryotic cells.e. All of the above are correct.arrow_forward
- Which of the following statements regarding DNA polymerases ineukaryotes is not correct?a. DNA polymerase α synthesizes a short RNA–DNA primer.b. DNA polymerases δ and ε synthesize most of the lagging and leading strands, respectively.c. Translesion-replicating DNA polymerases can replicate over damaged DNA.d. All of the above statements are correct.arrow_forwardA DNA probe with sequence TCAGGCTTCAG would bind most strongly to which of the following DNA fragments? a. AGTCCGAAGTC c. GACTTCGGACT b. TCAGGCTTCAG d. UGAGGCUUGAGarrow_forwardScientists can distinguish between DNA of different individuals, thus making this information useful in criminal investigations. The technique used is called __________. a. DNA fingerprinting b. CRISPR/Cas9 c. fluorescent in situ hybridization d. PCRarrow_forward
- in Cohen-Boyer's recombinant DNA procedure _______ must be used for both the bacterial DNA and the amphibian DNA _______. a. the same restrictions enzyme, so that the the restriction site are identical in the DNA of each species b. different restriction enzymes, so that the genes outside the restriction site are maintained c. the same restriction enzymes, to ensure that the newly formed DNA can replicate d. different restriction enzymes, to ensure that the newly introducted genes are maintained in the bacterial DNAarrow_forwardYou have extracted a long piece of DNA from a human cell and you want to extract the gene of interest for you to clone it. Assuming that you know the sequence of the DNA what methods can youuse to amplify the gene of interest? A. antibody purification B. polymerase chain reaction C. none of the above D. ligationarrow_forwardIn gene reflection, which is part of a designated vector that cannot be part of the original 1. plasmid? A. Foreign or transformed gene B. Ori site C. Multiple cloning site D. Resistance gene 2. Statement I: During ligation, a recombinant DNA technology have different meanings Statement II: Recombination can only be facilitated with DNA tools and genetic engineering. A. Both are correct. B. Both are false. C. Statement I is correct and Il is false. D. Statement I is incorrect and II is correct.arrow_forward
- Which of the following statements regarding DNA polymerases in eukaryotes is not correct? a. DNA polymerase α synthesizes a short RNA-DNA primer. b. DNA polymerases ε and δ synthesize most of the leading and lagging strands, respectively. c. Lesion-replicating DNA polymerases can replicate over damaged DNA. d. All of the above statements are correct.arrow_forwardAn individual’s unique set of_______ can be used in DNA profiling. a. DNA sequences c. SNPs b. short tandem repeats d. all of the abovearrow_forwardPlace the steps of sanger sequencing in order.A. A fluorescent laser excites the fragments and records the wavelength consistent with a single nucleotide. B. ddNTPs bind and stop chain extension.C. DNA fragments are separated by size through a capillary tube. D. DNA polymerase copies the target region of template DNA.E. The final nucleotide of each fragment is labeled with a fluorescent tag.arrow_forward
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