Why is Taq polymerase used in PCR rather than other DNA polymerases?a. Taq polymerase is a synthetic enzyme that produces DNA strands ata faster rate than natural polymerases.b. Taq polymerase is a heat-stable form of DNA polymerase that canfunction after exposure to the high temperatures necessary for PCR.c. Taq polymerase is easier to isolate than other DNA polymerases.d. Taq polymerase is the DNA polymerase commonly produced bymost eukaryotic cells.e. All of the above are correct.

The typical cycle of a PCR reaction includes a period of time at 95 degrees, 55 degrees, and 72 degrees. Match the temperature to the event that happens at that temperature.          -               95 degrees   55 degrees   72 degrees A. the DNA polymerase enzymes build new DNA at this temperature, starting from the 3' hydroxyl end of the annealed primers B. the pre-synthesized primers find their complementary sites on the template and attach via hydrogen bonds at this temperature C. the complementary strands of the DNA become separated at this temperature

A researcher is performing PCR to amplify a sample of DNA. Unfortunately, he forgot to add the DNA primer prior to starting the experiment. Which of the following results is he most likely to observe? a. The reaction will work, but at a significantly slower rate. b. The reaction will work, but the product will contain many undesired mutations. c. The reaction will work, but amplify a region that was not his target. d. The reaction will be completely unsuccessfu

A primer is laid down complementary to the DNA sequence TAGCAATCGCA to prime synthesis of a daughter strand. What will the sequence of the primer be? A. ATCGTTAGCGT B. ATGGTTAGGGT C. AUCGUUAGCGU D. AUCGTUAGCGT

Which of the following enzymes is NOT involved in DNA replication?   a. DNA Polymerase   b. Primase   c. Helicase   d. Phosphatase   e. DNA Ligase

Which of the following is TRUE with respect to electrophoresis? a. DNA is not attracted to either pole but in fact moves by diffusion through the TAE buffer in the agarose.  b. DNA can be attracted to either pole depending on the bases it is made of.  c. DNA is attracted to the negative pole.  d. DNA is attracted to the postive pole

Certain restriction endonucleases produce cohesive (sticky) ends.  This means that they: a. stick tightly to the ends of the DNA they have cut. b. cut both DNA strands at the same base pair. c.   make a staggered double-strand cut, leaving ends with a few nucleotides of single-stranded DNA protruding.   d. cut in regions of high GC content, leaving ends that can form more hydrogen bonds than ends of high AT content. e. cut in regions of high AT content, leaving ends that can form more hydrogen bonds than ends of high GC content.

DNA polymerase adds nucleotides to Select one: a. the 5′ end, and the leading strand elongates in the 3′ to 5′ direction b. the 3′ end, and the leading strand elongates in the 5′ to 3′ direction c. the 5′ end, and the leading strand elongates in the 5′ to 3′ direction d. the 3′ end, and the leading strand elongates in the 3′ to 5′ direction

You want to amplify a specific region of DNA during PCR. Your primers should   a Both should be an exact replica of the beginning of the specific gene you want to amplify b Both should be complementary to the beginning of the specific gene you want to amplify c One primer should be identical to the beginning of the gene sequence while the other is identical to the end of the gene sequence d One primer should be complementary to the beginning of the gene sequence while the other is complementary to the end of the gene sequence e One primer should be identical to the beginning of the gene sequence while the other is complementary to the end of the gene sequence

Some bacteria, through natural selection, have acquired some extremely potent enzymes that destroy viral DNA, thereby preventing the bacterial cell from becoming infected with the virus. These enzymes are called: Select one: O a. DNA polymerases O b. DNA ligases c. restriction endonucleases O d. restriction ligases