Place the steps of sanger sequencing in order. A.  A fluorescent laser excites the fragments and records the wavelength consistent with a single nucleotide. B.  ddNTPs bind and stop chain extension. C.  DNA fragments are separated by size through a capillary tube. D.  DNA polymerase copies the target region of template DNA. E.  The final nucleotide of each fragment is labeled with a fluorescent tag.

Q: Select all of the following that need to be in a polymerase chain reaction that will successfully…

A: Polymerase chain reaction (PCR) is a molecular technique used by scientists to multiply particular…

Q: Which drug would cause disruption to an amoeba's movement? (Note: these drugs are real and their…

A: The pseudopodia is formed by the actions of actin polymerization forming the microfilaments. The…

Q: DNA molecules with compatible sticky ends can be joined together by Select an answer and submit. For…

A: dna is the genetic material . it is a polymer of nucleotides , which are formed by nitrogen base ,…

Q: A DNA microarray is a slide that is dotted witha. mRNAs from a sample of cells.b. fluorescently…

A: Bio-informatics is the branch of biology that uses computers and statistics to analyse and record…

Q: The in situ hybridization method is based on Select one: a. labeling specific neuronal components…

A: Hybridization is the property of single-stranded nucleic acids to base-pair with their complementary…

Q: PCR is used to: Select one: a. Randomly break large pieces of DNA or plasmids into smaller…

A: PCR ( polymerase chain reaction ) is used to amplify a specific segment of DNA . Amplification is…

Q: The function of a restriction enzyme is to a. prevent the movement of DNA outside the nucleus b.…

A: Restriction enzymes:- these are the proteins and mostly isolated from prokaryotes. These enzymes…

Q: In next-generation sequencing, which of these advances allows for massively parallel sequencing?

A: Ans : Next Generation Sequencing (NGS) is the genome sequencing technology where we can determine…

Q: Slipped, mispaired DNA occurs when the DNA region has A. direct repeats. B.…

A: Slipped (SSM) strand mispairing is considered as a mutation process, in which occurs during the…

Q: Link the technique to the correct enzyme.…

A:

Q: ) Base excision repair requires polymerases. B.) In DNA repair by excision, the non-damaged strand…

A: Solution : Correct option is d

Q: A genetic map provides a. the sequence of the DNA in a genome. b. the relative position of genes…

A: The genome of an organism represents all the genetic information contained within the cells. This…

Q: Which of the following experimental results was NOT evidence that DNA is the genetic molecule rather…

A: Eukaryotes have linear DNA which is a double-chained helically twisted structure. it has a…

Q: Which of the following is FALSE about current Sanger dideoxy DNA sequencing procedures?   a. Chain…

A: An oligonucleotide primer is annealed to a single-stranded DNA template and expanded by DNA…

Q: Polymerase Chain Reaction, or PCR, can

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Q: With regard to dideoxy sequencing, which of the following statementsis false?a. The dideoxy…

A: Nucleotides are the molecules composed of a sugar moiety, phosphate group, and a nitrogenous base.…

Q: By mistake, you add two primers to your DNA sequencing reaction. The primers anneal on the same…

A: The correct answer is:B. The detector will detect two nucleotides at about 75% of the positions.

Q: In E.coli, the following enzymes do NOT synthesize the RNA primer for Okazaki fragments?

A: Introduction:- E.coli,like most bacteria, has  a single origin of replication on its chromosome. As…

Q: Which of the following is NOT true of DNA polymerase?   a.  DNA polymerase builds the new strand…

A: DNA polymerase is a type of enzyme found in all living things. Its primary function is to duplicate…

Q: PCR relies on the use of a pair of primers amplify a portion of DNA Which of the following…

A: The study of the make-up, structure, and interconnections of cellular molecules, that include…

Q: During proofreading, which of the following enzymes reads the DNA? a. primase b. topoisomerase c.…

A: BASIC INFORMATION  DNA  it stands for deoxyribo nucleic acid.  it is the genetic material of all…

Q: Sanger sequencing (aka the chain termination) a. is a method for for determining the sequence of…

A: Without a doubt!Identifying the order of nucleotides in a section of DNA may be accomplished by the…

Q: Match the substance to it's function. B. v Prevents supercoiling/Overwinding of the DNA A.…

A: Nucleosomes are the basic packing unit of DNA built from histone proteins around which DNA is…

Q: DNA Polymerase holoenzymes used for DNA replication recognizes   A. double-stranded sequences as…

A: DNA polymerase III holoenzyme (Pol III HE) is an enzyme that catalyzes elongation of DNA chains…

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A: Nucleic acids are large molecules that are made up of nucleotides that performs a various function…

Q: The CRISPR method is used in  a.DNA sequencing. b.DNA fingerprinting. c.genome editing. d.the…

A: CRISPER stands for clustered regularly interspaced short palindromic repeats. It is a group of DNA…

Q: If you repeat the okazaki experiment WITHOUT denaturing DNA, what would be the expected outcome? O…

A: The Okazaki experiment explained that the process of DNA replication is discontinuous and also…

Q: One common feature of all DNA polymerases is that they a. synthesize DNA in the 3′-to-5′…

A: One common feature of all DNA polymerases is that they synthesize DNA in the 5'-3' direction.

Q: The Meselson and Stahl experiment used a density label toa. determine the directionality of DNA…

A: Deoxyribonucleic acid is a particle made out of two polynucleotide chains that curl around one…

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A: Specific sequences in the entering DNA are recognized by restriction endonucleases, which then break…

Q: When we discuss PCR and other similar techniques, the term Tm is often used. This refers to a.The…

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Q: Choose the combination of answers that most accurately completes the statement.Which of the…

A: Three major steps are involved in polymerase chain reaction are denaturing, annealing and extension.…

Q: Which of the following is true?   a. In one technique, the DNA sequence can be determined using…

A: There are several techniques for determining the sequence of DNA, which is the specific order of…

Q: Your colleague made the table below comparing DNA Polymerase to RNA Polymerases. Which row is…

A: Transcription is the molecular process which involves the DNA sequences that are transcribed as…

Q: What would be the outcome if the primers used in a polymerase chain reaction have lower GC content…

A: PCR (polymerase chain reaction) is a technique used widely in biotechnology to amplify the DNA…

Q: Consider the following DNA sample: 5' CCGAGAACTTA 3' Draw a gel showing the following: 1. Sanger…

A: Q.Explanation:- Introduction:- Sanger sequencing is a method that determines the sequence of a DNA…

Q: Please answer the following question and explain Q1. If MacLeod and McCarty had accidentally…

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Q: Check all the statements that are TRUE regarding 16S or ITS (microbiome) illumina (NGS) sequencing…

A: The Illumina sequencing chemistry is extremely precise, with the great majority of bases scoring Q30…

Q: Which of the following is TRUE with respect to electrophoresis? a. DNA is not attracted to either…

A: Electrophoresis is defined as the motion of dispersed particles relative to a fluid under the…

Q: Certain restriction endonucleases produce cohesive (sticky) ends.  This means that they: a. stick…

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Q: The polymerase chain reaction requires the following except A. primers complementary to the ends of…

A: Introduction: A specific DNA segment can be amplified in a laboratory setting using the Polymerase…

Q: The CRISPR system may be used to manipulate the genome in human cells, because Cas9 can

A: CRISPER system can use Cas protein to manipulate the genome present in human cell.

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.