Place the steps of sanger sequencing in order. A.  A fluorescent laser excites the fragments and records the wavelength consistent with a single nucleotide. B.  ddNTPs bind and stop chain extension. C.  DNA fragments are separated by size through a capillary tube. D.  DNA polymerase copies the target region of template DNA. E.  The final nucleotide of each fragment is labeled with a fluorescent tag.

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A: Polymerase chain reaction (PCR) is a molecular technique used by scientists to multiply particular…

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A: The pseudopodia is formed by the actions of actin polymerization forming the microfilaments. The…

Q: Match the terms associated with tne polymerase chain reaction with their correct descriptions.

A: PCR is the polymerase chain reaction in which Denaturation, Annealing and Extension process takes…

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A: Bio-informatics is the branch of biology that uses computers and statistics to analyse and record…

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A: Hybridization is the property of single-stranded nucleic acids to base-pair with their complementary…

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Q: Choose the one answer that fits best. Which statement regarding PCR is NOT correct (videos)?   a.…

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Q: PCR is used to: Select one: a. Randomly break large pieces of DNA or plasmids into smaller…

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Q: In next-generation sequencing, which of these advances allows for massively parallel sequencing?

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A: DNA helicase is used during the DNA replication process where it binds to the double-stranded DNA…

Q: Link the technique to the correct enzyme.…

A:

Q: ) Base excision repair requires polymerases. B.) In DNA repair by excision, the non-damaged strand…

A: Solution : Correct option is d

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Q: Explain how electrophoresis separates DNA strands. a. How is a DNA fingerprinting test interpreted?…

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Q: Polymerase Chain Reaction, or PCR, can

A: It is a technique to amplify small segments of DNA.  It makes copies of a specific region of DNA. It…

Q: With regard to dideoxy sequencing, which of the following statementsis false?a. The dideoxy…

A: Nucleotides are the molecules composed of a sugar moiety, phosphate group, and a nitrogenous base.…

Q: describe in your own words what DNA barcoding is. Explain how PCR is used in this process

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Q: Choose the one answer that fits best.  Read carefully. Which of the following statements about the…

A: Option C is not correct-The plasmid used for transformation is called E.coli

Q: By mistake, you add two primers to your DNA sequencing reaction. The primers anneal on the same…

A: The correct answer is:B. The detector will detect two nucleotides at about 75% of the positions.

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A: DNA differs from RNA in having deoxyribose sugar while RNA contains ribose sugar. In RNA, uracil…

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A: DNA polymerase III holoenzyme (Pol III HE) is an enzyme that catalyzes elongation of DNA chains…

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A: CRISPER stands for clustered regularly interspaced short palindromic repeats. It is a group of DNA…

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A: The Okazaki experiment explained that the process of DNA replication is discontinuous and also…

Q: One common feature of all DNA polymerases is that they a. synthesize DNA in the 3′-to-5′…

A: One common feature of all DNA polymerases is that they synthesize DNA in the 5'-3' direction.

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Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.