Study Guide for Campbell Biology
Study Guide for Campbell Biology
11th Edition
ISBN: 9780134443775
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece, Martha R. Taylor, Michael A. Pollock
Publisher: PEARSON
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Chapter 18, Problem 2IQ
  1. a. Repressible enzymes usually function in _____ pathways. The pathway’s product serves as a(n) _____ to activate the repressor and turn off enzyme synthesis, thus preventing overproduction of the product of the pathway. Genes for repressible enzymes are usually switched _____ and the repressor is synthesized in a(n) _____ form.
  2. b. Inducible enzymes usually function in _____ pathways. Nutrient molecules serve as _____ to stimulate production of the enzymes necessary for their breakdown. Genes for inducible enzymes are usually switched _____ and the repressor is synthesized in a(n) _____ form.
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E. coli has five genes that code for enzymes that make tryptophan. These genes are regulated by a single promoter and transcribed as one long gene. The presence of tryptophan shut down the production of tryptophan by the cell by binding to the repressor. This changes the repressors shape allowing it to bind to DNA operator, blocking RNA polymerase and cutting off the production of tryptophan. a. Describe what would happen to the operon if some of the cells had a mutation on the repressor, not allowing it to bind with tryptophan. The repressor is described as an allosteric protein. What does this mean? b. Does the tryptophan model demonstrate an inducible or repressible operon? What is your evidence?*
a.  What is the function of operons in bacterial gene regulation? b.  Describe how a bacterial operon can be regulated by repressible proteins (such as the tryptophan operon).  Include in your description both the “on” and “off” states of the operon.  Key elements of your diagram should include: Co-repressor, Genes, mRNA, Operator, Operon, Promoter, Repressor, RNA polymerase.
. In the tryptophan operon of E. coli, what happens when tryptophan levels get too high? tryptophan is removed from its co-repressor, causing it to detach from the operator tryptophan is removed from its co-repressor, causing it to attach to the operator tryptophan is bound to its co-repressor, causing it to detach from the operator tryptophan is bound to its co-repressor, causing it to attach to the operator all of the above occur at equal rates when tryptophan levels get too high
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QCE Biology: Introduction to Gene Expression; Author: Atomi;https://www.youtube.com/watch?v=a7hydUtCIJk;License: Standard YouTube License, CC-BY