Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 11.1, Problem 4TQ
Summary Introduction
To review:
The effects of lambda mutants lacking endolysin and holin on their infection cycle.
Introduction:
Lambda is a bacteriophage that infects Escherichia coli. It consists of a linear double stranded genome that is packaged within an icosahedral phage capsid. The capsid is attached to the tail, that is, non-contractile. The tail fibers present at the end of the tail aid in the attachment of the phage to the host cell.
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M13 is a filamentous phage that infects the bacterium Escherichia coli. Infection with M13 is
not lethal. However, the infection causes turbid plaques in E. coli because infected bacteria
grow slower than the surrounding uninfected bacteria. This phage has been engineered to act
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illustration.
The RNA transcript of a region of T4 phage DNA contains the sequence 5’-AAAUGAGGA-3'. This sequence encodes three different polypeptides. What are they?
The figure above shows a schematic of genes and transcription control elements from phage λ. Use this figure as an aid to help you describe the molecular events involved in:
a) The establishment of lysogeny
b) The establishment of a lytic life cycle
Chapter 11 Solutions
Microbiology: An Evolving Science (Fourth Edition)
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- Consider the phase variation of flagella in Salmonella. Describe the phenotype of a cell in which hin is deleted. Will it be motile? Will it undergo phase variation? Which flagellin(s) will be used, if any? Name two bacterial defenses against bacteriophages and explain how they work How might a phage evolve resistance to the CRISPR host defense?arrow_forwardExplain in molecular terms how a bacterial cell is made lysogenic by a temperate phage such a lambdaarrow_forwardWhat is the difference between the lytic and lysogenic cycle of bacteriophages? In the lysogenic cycle, the phage DNA integrates itself into the host genome after penetration, and continues to biosynthesis when conditions change The host cell is not killed in the lysogenic cycle The lytic cycle occurs in the nucleus, while the lysogenic cycle occurs in the cytoplasm In the lysogenic cycle, penetration is achieved through phagocytosisarrow_forward
- Certain environmental conditions such as exposure to UV light areknown to activate lysogenic λ prophages and cause them to progressinto the lytic cycle. UV light initially causes the repressor protein to be proteolytically degraded. Make a flow diagram showing the subsequent events that lead to the lytic cycle. (Note: The xis gene codes for an enzyme that is necessary to excise the λ prophage from the E. coli chromosome. The enzyme integrase is also necessary for this excision.)arrow_forwardAfter lambda infects a cell, how does it decide to create a lysogen? Name the major cis and transplayers and their function.arrow_forwardThis question combines your knowledge of bacterial conjugationand the genetic regulation that directs thephage λ reproductive cycles. When researchers mix donor Hfr strains with recipient F– bacteria that are lysogenic for phage λ, the conjugated cells survive normally. However, if donor Hfr strains that are lysogenic for phage λ conjugate with recipient F– bacteria that do not contain any phage λ, the recipient cells often lyse, due to the induction of λ into the lytic cycle. Based on your knowledge of the regulation of the two reproductive cycles of phage λ, explain this experimental observation.arrow_forward
- One of the reasons why phage therapy has not been applied widely is that bacteria can become resistant to bacteriophages as well, through mutations in genes encoding for specific proteins. What would be a protein in the bacterial cell that, if mutated, would make that cell resistant to phage infection?arrow_forwardBacteriophage lambda is integrated in DNA of a host cell, which is exposed to UV light in a body of water in the environment. Describe the sequence of events that occurs in its life cycle. Briefly describe the role of the Lambda repressor (cI) and cII activator to regulate the switch between the lysogenic and lytic cycles of phage Lambda.arrow_forwardCertain mutations called amber in bacteria and viruses result in premature termination of polypeptide chains during translation. Many amber mutationshave been detected at different points along the gene thatcodes for a head protein in phage T4. How might this system be further investigated to demonstrate and support the concept ofcolinearity?arrow_forward
- when various strains of lambda phage are seeded on a lawn of e.coli, they can form clear or turbid plaques. Explain the difference between the two types of plaques. can all bacteriophage form clear and turbid plaques?arrow_forwardWhat advantages might a phage gain by being capable of lysogeny?arrow_forwardDescribe how the lambda phage controls the lytic and lysogenic cycles. Include cro, cI, cII, and cIII in your description. Which of these proteins is responsible for the initiation of lysogeny or lytic pathways?arrow_forward
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