Genetics: From Genes to Genomes
6th Edition
ISBN: 9781259700903
Author: Leland Hartwell Dr., Michael L. Goldberg Professor Dr., Janice Fischer, Leroy Hood Dr.
Publisher: McGraw-Hill Education
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Chapter 11, Problem 18P
Summary Introduction
To determine:
The ways by which a prosecutor use DNA from the seed pods to build a case against the defendant.
Introduction:
DNA fingerprinting is defined as the method in which variable elements present in the sequence of DNA are isolated and identified. The DNA is obtained from a sample of cells such as skin, blood, or hair cells. They are a rich source of DNA.
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Examine the gel from a Rape investigation below. If you were the DNA analyst you would conclude that:
DNA size
markers
blood
sexual
assault
evidence
samples
victim
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A
suspect
B
female
fracțion
male
fraction
1 2 3 4 5 6
A. Suspect B is excluded as the source of the evidence, but Suspect A cannot be excluded.
B. Both Suspects A and B are excluded as the source of the evidence.
C. Suspect A is excluded as the source of the evidence, but Suspect B cannot be excluded.
D. Suspect B cannot be excluded as a source of the evidence. The results with Suspect A are inconclusive.
E. Neither Suspect A or B can be excluded as a source of the evidence.
Brenda is a junior student in the biomedical program at her school. She is starting the PCR genetic testing lab activity. She is about to obtain her DNA sample but doesn’t want like the taste of NaCl solution. Her friend, Mark, let her use some of his DNA. What laboratory tule did the students break?
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Below is an image of the results of a gel electrophoresis experiment.
Lanes 1-4 contain amplified DNA fragments and Lane 5 contains the
DNA size marker (DSM). From this image and by only using Lanes 1-
4 which lane matches each description below?
a) This lane contains the largest DNA fragment:
b) This lane contains the smallest DNA fragment:
c) This lane contains a 2.5 kbp DNA fragment:
42
| ||
||| | ||
12 kb
9.5 kb
6.5 kb
4 kb
2 kb
***
Chapter 11 Solutions
Genetics: From Genes to Genomes
Ch. 11 - Choose the phrase from the right column that best...Ch. 11 - Would you characterize the pattern of inheritance...Ch. 11 - Would you be more likely to find single nucleotide...Ch. 11 - A recent estimate of the rate of base...Ch. 11 - If you examine Fig. 11.5 closely, you will note...Ch. 11 - Approximately 50 million SNPs have thus far been...Ch. 11 - Mutations at simple sequence repeat SSR loci occur...Ch. 11 - Humans and gorillas last shared a common ancestor...Ch. 11 - In 2015, an international team of scientists...Ch. 11 - Using PCR, you want to amplify an approximately 1...
Ch. 11 - Prob. 11PCh. 11 - The previous problem raises several interesting...Ch. 11 - You want to make a recombinant DNA in which a PCR...Ch. 11 - You sequence a PCR product amplified from a...Ch. 11 - Prob. 15PCh. 11 - The trinucleotide repeat region of the Huntington...Ch. 11 - Sperm samples were taken from two men just...Ch. 11 - Prob. 18PCh. 11 - a. It is possible to perform DNA fingerprinting...Ch. 11 - On July 17, 1918, Tsar Nicholas II; his wife the...Ch. 11 - The figure that follows shows DNA fingerprint...Ch. 11 - Microarrays were used to determine the genotypes...Ch. 11 - A partial sequence of the wild-type HbA allele is...Ch. 11 - a. In Fig. 11.17b, PCR is performed to amplify...Ch. 11 - The following figure shows a partial microarray...Ch. 11 - Scientists were surprised to discover recently...Ch. 11 - The microarray shown in Problem 25 analyzes...Ch. 11 - The figure that follows shows the pedigree of a...Ch. 11 - One of the difficulties faced by human geneticists...Ch. 11 - Now consider a mating between consanguineous...Ch. 11 - The pedigree shown in Fig. 11.22 was crucial to...Ch. 11 - You have identified a SNP marker that in one large...Ch. 11 - The pedigrees indicated here were obtained with...Ch. 11 - Approximately 3 of the population carries a mutant...Ch. 11 - The drug ivacaftor has recently been developed to...Ch. 11 - In the high-throughput DNA sequencing protocol...Ch. 11 - A researcher sequences the whole exome of a...Ch. 11 - As explained in the text, the cause of many...Ch. 11 - Figure 11.26 portrayed the analysis of Miller...Ch. 11 - A research paper published in the summer of 2012...Ch. 11 - Table 11.2 and Fig. 11.27 together portray the...Ch. 11 - The human RefSeq of the entire first exon of a...Ch. 11 - Mutations in the HPRT1 gene in humans result in at...Ch. 11 - Prob. 44P
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- Is PCR technique can help solved crimes? If yes, how? If no, why? [Explain it in 10-15 sentences]arrow_forwardDNA dragnets have been so successful that some people have suggested that DNA samples of everyone should be stored at birth, so a profile could be made for anyone at any time. A. Do you think this is a good idea or not? And, B. do you think it useful or ethical for the FBI to store DNA samples from people who have been arrested but not yet convicted of a crime? Answer both questions,arrow_forwardWhat is the role of Agrobacterium tumefaciens in the production of transgenic plants? Question 15 options: Tomatoes get increased shelf life when exposed to the beneficial bacteria Agrobacterium tumefaciens. Transgenic plants are all designed to be resistant to the disease causing bacteria Agrobacterium tumefaciens. Agrobacterium tumefaciens is a bacteria that is used to move desired genes into the plant cells. Agrobacterium tumefaciens is a bacteria that produces Bt toxins that kill small mammals.arrow_forward
- Which of the following statements regarding DNA fingerprinting is false?a) DNA fingerprinting cannot be used for paternity testingb) DNA profile using STR (short terminal repeats) is unique to an individualc) PCR is used for DNA profilingd) Forensic analysis makes use of SNPs (single nucleotide polymorphisms) in coding sequences to distinguish between individualsarrow_forwardDiscuss the principle of DNA fingerprinting. How would you use the technique to find out a criminal in forensic studies ? Explain.arrow_forwardState the five basic steps of DNA fingerprinting using the RFLP method. Why do you think the PCR method is of more use in crime scene investigations?arrow_forward
- Please answer these two questions regarding PCR: a) Why do you need to perform PCR on DNA obtained from a crime scene? b) Why so forensic labs analyze non-coding DNA rather than genes?arrow_forwardFor what purpose is DNA fingerprinting used A) to sequence DNA from bacteria B) to separate DNA fragments C) to identify individuals who have committed crimes D) to identify single nucleotide polymerasearrow_forwardWhat are three major limitations of forensic DNA profiling?arrow_forward
- Why can a DNA sample not be a reliable source of evidence?arrow_forward1. These gels represent different cases. Do either of these gels eliminate the alleged father from being the real father? Do either of these gels prove the alleged father is the real father? 0 ITE Crime Scene aaaava ... ... ... .. yaaava ... 000 0.. ..... 2. You isolate DNA from a crime scene and a suspect and then analyze a variable region by PCR and gel electrophoresis. Your results look like this Suspect From this you conclude A. The suspect committed the crime B. The suspect was at the crime scene C. The suspect could not have been at the crime scene D. The suspect did not commit the crime E. The suspect could have been at the crime scene, but more genes must be examinedarrow_forwardWhy is ripe banana a better sample compared to an unripe banana for DNA extraction ?arrow_forward
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