Please answer these two questions regarding PCR:   a) Why do you need to perform PCR on DNA obtained from a crime scene? b) Why so forensic labs analyze non-coding DNA rather than genes?

There are four possible observations that suggest an error occurred in a PCR reaction: a) Sequence errors in the amplicon, b) the amplicon is the wrong size, c) there is no 6. Application: e are four possible observations that suggest an error occurred in a PCR reaction: 7 Sequence errors in the amplicon, b) the amplicon is the wrong size, c) there is ho amplicon, or d) there are multiple, non-specific amplicons. At tô thể possible cause, write which error is most likely associated with the cause (the first one is done as an example): Cause Would result in the following error Unbalanced nucleotide concentrations a) Sequence errors in the amplicon Annealing temperature too low Poor primer specificity (wrong sequence) Template DNA degraded Contamination of reaction tubes or solutions Lack of Mg2+ in reaction mixture Pick one cause and result pair and discuss in detail on the next page why one would lead to the other:

You are preparing to amplify a DNA sample using PCR and add the following to your set-up: forward primers, no dNTPs, and Taq polymerase.  What will be the end result of your PCR?   a) PCR would proceed normally b) Non-specific PCR of random templates will occur c) The reaction will cease after a few cycles d) The PCR reaction will not occur.

For what purposes is DNA extraction done? (give at least 3 purposes for which you may need to extract DNA)