Please answer these two questions regarding PCR:   a) Why do you need to perform PCR on DNA obtained from a crime scene? b) Why so forensic labs analyze non-coding DNA rather than genes?

Q: Is the given statement that the first step of PCR process involves heating of DNA solution TRUE or…

A: The reaction that is used to make the duplicates of a particular DNA segment is known as polymerase…

Q: Which of the following enzyme is used in PCR?a) Taq DNA polymeraseb) HRPc) EcoRId) EcoRII

A: Polymerase chain reaction (PCR) is a laboratory technique, which is used to make multiple copies of…

Q: Explain why a quantitative PCR analysis cannot determine the size of the initial template sequence…

A: The combination of the probe-based and intercalating dye-based methods brings the potential…

Q: PCR has many useful applications. However, an incorrect application of PCR is: a) Amplification of…

A: DNA or Deoxyribonucleic acid is a macromolecule which is responsible for the transfer of hereditary…

Q: Define the term PCR.

A: Dr.Kary Mullis developed the polymerase chain reaction (PCR) in 1983. The enzyme utilised in this…

Q: If you wanted to prepare only one PCR reaction, how much of each reagent would you add to the PCR…

A: Standard PCR reagents include a set of appropriate primers for the desired target gene or DNA…

Q: What phase of PCR (exponential, linear, or stationary) is analyzedto quantitate the amount of DNA or…

A: The genetic entity of the organism is mainly composed of nucleic acids. The nucleic acids DNA and…

Q: Why are entire genomes not used for DNA profiling?

A: DNA profiling is the process of characterizing a sequence of DNA obtained from a person. It is also…

Q: If a DNA sample was GC-rich, what would you need to do to adjust your PCR reaction and why?

A: DNA is a sequence of nucleotides with four different nitrogenous bases like adenine, guanine,…

Q: Is the given statement that the first step of PCR process involves heating of DNA solution TRUE or…

A: PCR process is a straightforward however valuable methodology in molecular science called the…

Q: There are four possible observations that suggest an error occurred in a PCR reaction: a) Sequence…

A: PCR reaction is a method used to amplify a small sample into millions of small ones which help the…

Q: Recombinant DNA construction involvesa) Cleaving DNA with restriction endonuclease and joining with…

A: Genetic engineering is the branch of biological science that can input alteration of DNA in an…

Q: What is the difference between PCR and real-time PCR, and how do you explain it?

A: The Polymerase chain reaction (PCR) is a technique that allows researchers to rapidly make millions…

Q: What are the reasons why there needs to be more than 10 cycles in the PCR process? Name 3 reasons.

A: DNA sequences can be amplified in vitro using the PCR (Polymerase Chain Reaction) method. A…

Q: PCR has revolutionalized biotechnology. Discuss three advantages and three disadvantages of PCR…

A: PCR or Polymerase Chain Reaction is the technique which is used for making many copies of a specific…

Q: What is PCR bias? Why is it undesirable?

A: Answer: Introduction: Polymerase chain reaction (PCR) is a process extensively used to quickly form…

Q: After four cycles of PCR, which products predominate? Explain why.

A: Polymerase chain reaction (PCR) is a type of genetic analysis in which the nucleotide sequence of…

Q: Which is used for cloning eukaryotic genes but not prokaryotic genes?a) Restriction enzymesb) DNA…

A: Restriction enzymes are the enzymes that cleave the deoxyribonucleic acid (DNA) at specific sites…

Q: Each PCR cycle has three steps: DNA sample denaturation, primer annealing, and elongation/extension…

A: Polymerase chain reaction (PCR) is a simple technique to amplify a DNA template to produce specific…

Q: The PCR reaction contains deoxynucleotide triphosphates (dNTPs) in order to construct new DNA. There…

A: PCR is a sensitive technique which helps in making rapid amplification of a specific DNA segment.…

Q: Why is it necessary to use a special DNA polymerase(Taq polymerase) in PCR?

A: PCR (Polymerase Chain Reaction) is a technique that is used to amplify specific DNA sequences. The…

Q: For PCR, what property of DNA does temperature influence? Why do the “denaturation” and “annealing”…

A: PCR stands for polymerase chain reaction. It is an in-vitro laboratory technique used for generating…

Q: What are the reagents used for Reverse Transcriptase PCR (RT-PCR) and Reverse Transcriptase–Real…

A: Introduction Reagents are substances used for the detection or determination of another substance,…

Q: What are the criteria for “good” primers in a PCR reaction?

A: Polymerase chain reaction (PCR) is the method that is widely used to make billions of copies of a…

Q: Why Touch-down PCR technique is better than the Gradient PCR technique? Also, from those techniques…

A: The PCR or polymerase chain reaction is a technique to enhance or amplify DNA sequences in vitro by…

Q: Describe the possible outcome of a PCR experiment in which (a) one of the primers is inadvertently…

A: The polymerase Chain reaction is molecular biology in vitro technique to make multiple copies of a…

Q: Will any of the PCR reaction components (e.g., dNTPs, primer) become limiting before the product is…

A: Polymerase Chain Reaction (PCR): It is a molecular technique for making multiple copies of DNA…

Q: Why is the use of a heat-stable DNA polymerase important to the success of PCR?

A: The polymerase chain reaction (PCR) is a method used to amplify the deoxyribonucleic acid (DNA) of…

Q: Lane M 1 2 3 4 5 Content DNA ladder Replicate 1 Replicate 2 Replicate 3 Negative control Positive…

A: PCR stands for polymerase chain reaction. PCR is used to amplify nucleic acid segments for further…

Q: How does RT-PCR differ from traditional PCR?

A: Polymerase Chain Reaction (PCR) is a technique that gives us multiple copies of desired DNA…

Q: What is the nature of the primers used in RT-PCR tests? (i.e. are they DNA primers or RNA primers?)

A: In RT-PCR, the RNA sample obtained from a virus is converted into DNA, and the DNA is amplified and…

Q: What are the reasons why there needs to be more than 10 cycles in the PCR process? Name 2 reasons.

A: PCR (Polymerase Chain Reaction) is a technique used to amplify DNA sequences in vitro. The process…

Q: Briefly explain how the polymerase chain reaction is used to amplify a specific DNA sequence. What…

A: PCR which stands for polymerization chain reaction is a rapid and extremely sensitive analytical…

Q: What is the purpose of adding a primer to a PCR reaction? Is this primer made up of DNA or RNA…

A: The polymerase chain reaction is a method that is widely used to rapidly make millions of copies of…

Q: In PCR, the size of the product is based on A the position of the primers. B) the amount of DNA you…

A: PCR is a method widely used to rapidly make millions to billions of copies of a  specific DNA…

Q: Explain why a positive control and negative control are included in PCR experiments. ​Explain the…

A: PCR is a laboratory technique that is used for making large number of copies of a target DNA…

Q: The current state-of-the-art in forensic DNA profiling involves the PCR-amplification and analysis…

A: PCR is a process in which millions of copies of a specific sequence of DNA can be made in a matter…

Crime Scene Investigation

A crime scene refers to a place where a crime has occurred. The purpose of crime scene investigation (CSI) is to analyze the complete scene and catch the culprit based on the pieces of evidence collected from the place of crime. The people involved in this process are referred to as crime scene investigators.