Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 6, Problem 9RQ
Summary Introduction
To review:
The growth curve of virus and its difference from bacterial growth curve.
Introduction:
The culture of virus in a laboratory requires double culturing of the virus with its specific host. The bacteriophages require specific bacterial cell culture to proliferate and animal virus are grown in tissue cultures. The replication of viruses in a culture also follows a specific growth pattern.
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Microbiology: An Evolving Science (Fourth Edition)
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- Describe two mechanisms a virus might use to prevent the induction of apoptosis in an infected cell.arrow_forwardCoronaviruses have a positive-sense single stranded RNA genome. a.) What is the genome replication strategy of this category of viruses? b.) How do you think this feature affects the stability of the viral genome? (hint use figure below) Mutation rates in different types of viruses. Estimates obtained for viruses belonging to the major indicated groups are shown as a function of genome size. This reveals an effect of genetic material (RNA/DNA) and genome size on viral mutation rates.arrow_forwardSome retroviruses that have a wide host range have been developed for use in treatment of diseases or syndromes. These viruses do not result in a productive infection because they lack critical genes for viral replication, but they do carry DNA sequences that allow them to be packaged into infectious particles. Once in the host cell the viral DNA is released and integrates into the cell's genome. The advantage to this strategy is that the delivery of the DNA is very efficient. What is one possible problem of using a virus that can integrate into the human nuclear genome? Multiple Choice The virus carries genes that will interfere with host cell activities. The viral DNA sequences could still cause a disease. Since the viral DNA sequences no longer contain essential genes for viral replication the host cell may not be able to replicate either. The integration could disrupt an essential gene or genes.arrow_forward
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- How do cellular receptors for viruses come about? does host species that a particular virus infects evolve to have the viral receptors? would the above offer any survival and/or reproductive advantages to the host? how likely does the virus mutate so it would recognize (or fit) a particular exiting surface protein on the target cells of the host?arrow_forwardWhen a virus infects eukaryotic cells, one fate of the cell is lysis. List and briefly describe two other potential fates.arrow_forwardWhat defines a virus' host range? Know the multiplication steps (in order) for a virus (both bacteriophage and animal viruses). What happens at each step? What is the major difference between the steps in animal viruses vs. bacteriophages? Describe the lytic and lysogenic cycles of bacteriophage infection. What are the different types of viral genomes possible? What are the differences between entry and exit for enveloped vs naked viruses? o i.e. fusion, endocytosis, budding or exocytosis, lysis/bursting/rupturing, Explain how viruses are cultured. Why would viruses need to be cultured? What is a latent viral infection? What are cytopathic effects (know examples). Know characteristics of viroids and prions. What are they? What kinds of diseases can they cause (know examples for prions) How can viral infections be treated? bparrow_forward
- After a positive strand RNA virus ( Such as Coronaviruses) enters the host cell, the RNA is translated by ribosomes to create proteins associated with the virus (Such as, for example, the surface antigens like E and Spike proteins). How then, would the genetic material itself be replicated to be incorporated into the new viruses?.arrow_forwardIdentify the numbered steps of this viral life cycle/replication cycle depicted here.arrow_forward‘’Viruses cannot be grown in standard microbiological culture such as broth and agar. They need to be cultured in the presence of a suitable host such as prokaryotic cells (easiest to grow in the lab), plants and animals because they are unable to reproduce independently in living cells’’.2.2 What are the changes that are brought by a virus to a cell? In your response, make reference to the cytopathic effect (CPE).arrow_forward
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