Microbiology: An Evolving Science (Fourth Edition)
Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 6, Problem 7RQ
Summary Introduction

To review:

The plate count method for enumeration of bacteriophages and its modification for the measurement of animal and oncogenic viruses.

Introduction:

Viruses are dependent on host cells for their survival and reproduction. They cannot be grown in artificial culture medium due to their inability to replicate independently. However, certain manipulations in culturing method can be useful to detect and enumerate the number of virus in a given sample.

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During the incubation step of the plaque assay, viral replication leads to host cell lysis. This allows the freshly-released virions to diffuse through the media and infect new hosts. The amount of agar added to the top agar is 7 g per liter rather than the 16 g of agar per liter for most solid media, including the bottom agar used in this assay. How would it change the plaque assay results if bottom agar was used in place of the top agar in this assay? a- The diameter of the plaques would be smaller. b- The diameter of the plaques would not change. c- The diameter of the plaques would be larger. (2) A researcher measured the viral titer of PhiX174 in fish tank water, untreated sewage water, and unpasteurized apple juice. Which sample do you predict will have the highest titer on this page? a- Fish tank water b- Unpasteurized apple juice c- Untreated sewage water
Which of the following is TRUE when one assay bacteriophage titers? You should:   a) first mix the phages with a live bacterial culture and then pour the mixture on the agar plate   b) directly add the phage dilution onto the surface of an agar plate   c) add tryptic soy broth to the phage dilution and incubate overnight   d) incubate a phage solution with live bacterial cells for several minutes. You must add soft agar to the mixture before pouring the content on the agar plate
You infect bacteria with two phages during a cis test. This produces a lysate that contains phage particles. Before you can count outcomes, you need to work out the amount of phage to use in subsequent experiments. You start by making two ten- fold serial dilutions of the lysate and then add 1mL of the final diluted lysate to a plate of permissive strain e.coli. You observe 10 plaques. How many phage per mL were present in the original lysate? a. 10 phage/mL o b. 3,300 phage/mL c. 10,000 phage/mL o d. 1,000 phage/mL e. 33 phage/mL
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