Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 6, Problem 6RQ
Summary Introduction
To review:
The differences between the replication cycles of DNA and RNA viruses and their advantages and disadvantages.
Introduction:
The genome of viruses can consist of DNA (deoxyribonucleic acid) or RNA (ribonucleic acid).Depending on the nature of their genetic material, the replication of the virus varies. Another important feature is the strandedness of the genome which can be single stranded or double stranded.
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Match each of the following replication classes of viruses with the one description that best
describes it.
This virus class is composed of
segmented and non-segmented
helical viruses that carry an
RdRp within the virion into the
cell. Example: Rabies virus
These viruses are non-
enveloped, icosahedral, have
segmented genomes, and carry
their own RNA-dependent RNA
polymerase. The viral genome
is copied following assembly.
Example: Reovirus
This is the only class of RNA
viruses whose genome is
replicated by cellular enzymes
and not an RNA-dependent
RNA polymerase.
Cellular DNA polymerases
replicate this virus's non-
integrated genome, although
transcription is not the initial
event that takes place in the
process. Example:
Parvoviruses
In this class of viruses,
transcription occurs before
genome replication because
viral proteins are involved in
replicating the genome.
Example: Adenovirus
This class of viruses forms
circular DNA and creates pre-
genomic RNA using host RNA
polymerases.…
Coronaviruses have a positive-sense single stranded RNA genome.
a.) What is the genome replication strategy of this category of viruses?
b.) How do you think this feature affects the stability of the viral genome? (hint use figure below)
Mutation rates in different types of viruses. Estimates obtained for viruses belonging to the major indicated groups are shown as a function of genome size. This reveals an effect of genetic material (RNA/DNA) and genome size on viral mutation rates.
1)What are the difference between RNA and DNA?
2)Describe steps of viral replication, what are the differences in the replication between RNA and DNA viruses ?
Chapter 6 Solutions
Microbiology: An Evolving Science (Fourth Edition)
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- As a general rule, viruses are not considered to be alive based on several reasons. One such reason is that some viruses use RNA as their genetic material instead of DNA. Which of the five biological themes does this violate? Briefly explain why.arrow_forwardWhat defines a virus' host range? Know the multiplication steps (in order) for a virus (both bacteriophage and animal viruses). What happens at each step? What is the major difference between the steps in animal viruses vs. bacteriophages? Describe the lytic and lysogenic cycles of bacteriophage infection. What are the different types of viral genomes possible? What are the differences between entry and exit for enveloped vs naked viruses? o i.e. fusion, endocytosis, budding or exocytosis, lysis/bursting/rupturing, Explain how viruses are cultured. Why would viruses need to be cultured? What is a latent viral infection? What are cytopathic effects (know examples). Know characteristics of viroids and prions. What are they? What kinds of diseases can they cause (know examples for prions) How can viral infections be treated? bparrow_forwardMatch each stage of the viral replication cycle with the one definition that best describes it. ¡¡¡ The stage at which nascent virions leave the cell The virus adheres to the surface of the cell The viral nucleic acid is released into the cell The stage at which virions become infectious The construction of new virions composed of viral proteins and the replicated genome The stage at which the viral genome is copied The virus passes into the cell from the extracellular environment 1. Attachment 2. Replication 3. Maturation 4. Release 5. Penetration 6. Uncoating 7. Assemblyarrow_forward
- Viruses are made up of the same biomolecules as cellular organisms (DNA, RNA, proteins, lipids, etc.), which are synthesized through enzymatic dehydration reactions from building blocks and disassembled by enzymatic hydrolysis. They infect cells and replicate themselves using resources from the host cells. Scientists discovered a previously unknown strain of virus in the tropical Pacific Ocean and it can infect higher-order eukaryotes. Initial analysis revealed that the virus is mainly composed of nucleic acids, proteins, and lipids. Chemical analysis after complete hydrolysis revealed the following building blocks (by weight): Chemical components Nucleosides Amino acids Lipids, phosphates, and carbohydrates Percentage by weight 20 30 50 Analysis of percentages of nucleosides yielded the following results (by molar ratio): Nucleosides A G T C U Percentage by mole 19 29 19 29 4 Which of the following events mostly involves hydrolysis reaction after the virus infects…arrow_forward27. Class VI animal viruses include HIV, Based on the type of viral genome, how is this class distinguished from the other classes of animal virus? A) It has double stranded RNA. B) It has single stranded RNA that serves as mRNA (positive sense). C) It has single stranded RNA that serves as a template for the synthesis of DNA. D) It has single stranded RNA that serves as a template for mRNA (anti-sense). does HIVarrow_forwardHow do cellular receptors for viruses come about? does host species that a particular virus infects evolve to have the viral receptors? would the above offer any survival and/or reproductive advantages to the host? how likely does the virus mutate so it would recognize (or fit) a particular exiting surface protein on the target cells of the host?arrow_forward
- Define the terms "latency" and "lysogeny" as they refer to viruses. Describe at least TWO ways these two processes are similar. Describe at least TWO ways these two processes are different.arrow_forwardCompare how retroviruses solve the problem of replicating an RNA genome in a eukaryotic host with how other types of RNA viruses solve this same problem.arrow_forwardWhich of the following represents the correct stages (in order) of VIRAL replication? a-Replication, assembly, entry, attachment, lysis b-Lysis, assembly, entry, attachment, replication c-Attachment, entry, replication, assembly, lysis d-Lysis, assembly, replication, entry, attachmentarrow_forward
- Discuss the following statement: “Viruses exist in the twilight zone of life: outside cells they are simply dead assemblies of molecules; inside cells, however, they are alive.”arrow_forwardThe RNA genomes of some RNA viruses resemble the messenger RNA (mRNA) of their eukaryotic hosts. What advantage would an RNA virus gain by having this type of genome?arrow_forwardDescribe in general terms the strategy used by single-stranded (ss) DNA viruses to synthesize their nucleic acids and proteinsarrow_forward
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