Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 6, Problem 8RQ
Summary Introduction
To review:
The isolation of virus and its difference from bacterial isolation.
Introduction:
Virus are host dependent acellular organisms. They do not have active
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How does PCR help in the identification of the virus from patients?
What technique could be used to confirm the presence or absence of
plasmids in the donor, recipient and transconjugant strains?
Which of the following is TRUE when one assay bacteriophage titers? You should:
a) first mix the phages with a live bacterial culture and then pour the mixture on the agar plate
b) directly add the phage dilution onto the surface of an agar plate
c) add tryptic soy broth to the phage dilution and incubate overnight
d) incubate a phage solution with live bacterial cells for several minutes. You must add soft agar to the mixture before pouring the content on the agar plate
Chapter 6 Solutions
Microbiology: An Evolving Science (Fourth Edition)
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- Solution B that was used during the plasmid isolation contains 0.2 M NaOH (see practical manual). Which effect does NaOH have on E. coli DNA A) It denatures genomic DNA and plasmid DNA. B) It denatures genomic DNA and but not plasmid DNA. C) It denatures plasmid DNA but not genomic DNA. D) It denatures neither genomic DNA nor plasmid DNA. E) This is unpredictable.arrow_forwardSolution B that was used during the plasmid isolation contains 0.2 M NaOH (see practical manual). Which effect does NaOH have on E. coli DNA A) It denatures genomic DNA and plasmid DNA. B) It denatures genomic DNA and but not plasmid DNA. C) It denatures plasmid DNA but not genomic DNA. D) It denatures neither genomic DNA nor plasmid DNA. E) This is unpredictable. Which statement on the migration of DNA fragments through agarose gels is false A) Small fragments migrate faster than larger fragments because they can move faster through the agarose pores. B) Large fragments migrate faster than small fragments because they carry more negative charges. C) DNA fragments migrate towards the positive pole. D) Supercoiled DNA may migrate significantly different through the gel than linear DNA of equal size. E) The higher the agarose concentration the better the separation of smaller fragments as compared to larger fragments.arrow_forwardDuring the incubation step of the plaque assay, viral replication leads to host cell lysis. This allows the freshly-released virions to diffuse through the media and infect new hosts. The amount of agar added to the top agar is 7 g per liter rather than the 16 g of agar per liter for most solid media, including the bottom agar used in this assay. How would it change the plaque assay results if bottom agar was used in place of the top agar in this assay? a- The diameter of the plaques would be smaller. b- The diameter of the plaques would not change. c- The diameter of the plaques would be larger. (2) A researcher measured the viral titer of PhiX174 in fish tank water, untreated sewage water, and unpasteurized apple juice. Which sample do you predict will have the highest titer on this page? a- Fish tank water b- Unpasteurized apple juice c- Untreated sewage waterarrow_forward
- What is pGLO plasmid? How has it been engineered so that the cells that carry it can be detected? Attach a map of it.?arrow_forwardDescribe how would you determine the following using a real-time PCR instrument. A) The quantity and quality of a DNA sample. B) Whether an individual had cystic fibrosis from a genomic DNA sample. C) If several bacterial pathogens were present in DNA extracted from a food sample.arrow_forwardBased on the image below of a Kirby-Bauer assay, this bacteria is most susceptible to which antibiotic? Write the letter of the antibiotic below. G. B. E.arrow_forward
- Explain what must be done to identify RNA viruses using genetictechniques.arrow_forwardWhat is the purpose of using Triton X in hemolytic assay.arrow_forwardWhich of the following statements is true about a vector?a) Plasmids, phages can be used as vectorsb) All vectors are plasmidsc) Cyanobacteria can also be used as vectorsd) Fungi can also be used as vectorsarrow_forward
- 1. Download the complete nucleotide sequences of the following 11 isolates of Tomato leaf curl virus, with the NCBI reference numbers as indicated below.a) NC_003896.1b) NC_003897.1c) NC_010313.1d) NC_004153.1e) NC_003898.1f) NC_003891.1g) NC_055126.1h) NC_009031.1i) NC_009030.1j) NC_005842.1k) NC_005032.12. Run Multiple Sequence Alignment (MSA) using appropriate bioinformatics tool/program i.e. Clustal Omega. Upload or copy-paste directly all the 11 nucleotide genome sequences of the sampled ToLCV isolates. Apply default parameters.Questions:1. How many sub-clusters or sub-grouping of the ToLCV isolates could be generated? 2. Relate the sub-grouping to the genetic relationships among isolates/between subgroups? More similar isolates, divergence or less similar/related? 3. Any isolate that represent the predicted ancestral viral genome (no evident data to support a mutation event from the root/ancestral viral genome) - Oldest isolate (genome) sequence 4. Youngest or most evolved…arrow_forwardResults from a Kirby Bauer antibiotic assay on a Gram-negative bacterial culture are described as follows: A) the bacterium is resistant to penicillin, an antibiotic that targets synthesis of the peptidoglycan cell wall and B) the bacterium is resistant to tetracycline, an antibiotic that targets the small subunit of the ribosome, inhibiting protein synthesis. Which of the results represents intrinsic resistant and which represents acquired resistants?arrow_forwardWhy are the following reagents used? Neutralizing solution (Plasmid isolation) Isopropanol (Plasmid isolation) RNase (isolation of genomic DNA)arrow_forward
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