Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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Considering counting rules, calculate the initial titre of the sample viral stock if the following plaques
were counted in a plaque assay. Show clear sample calculations.
Table 1: Plaque assay counts for lambda phage stock titre enumeration of average concentration
(PFU/mL)
Total dilution of viral stock
1/13500000
1/135000000
1/135000000
plated
Volume diluted viral stock
0.1
0.1
0.1
plated (mL)
PFU replicate 1
TNTC
275
25
Calculated concentration
(PFU/mL) replicate 1
PFU replicate 2
TNTC
239
23
Calculated concentration
(PFU/mL) replicate 2
Average concentration
(PFU/mL)
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Transcribed Image Text:Considering counting rules, calculate the initial titre of the sample viral stock if the following plaques were counted in a plaque assay. Show clear sample calculations. Table 1: Plaque assay counts for lambda phage stock titre enumeration of average concentration (PFU/mL) Total dilution of viral stock 1/13500000 1/135000000 1/135000000 plated Volume diluted viral stock 0.1 0.1 0.1 plated (mL) PFU replicate 1 TNTC 275 25 Calculated concentration (PFU/mL) replicate 1 PFU replicate 2 TNTC 239 23 Calculated concentration (PFU/mL) replicate 2 Average concentration (PFU/mL)
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