Question: Genetically modified animal that might be approved for human consumption is a super “muscly” pig made by the inactivation of the myostatin gene. During normal development, the myostatin protein prevents the overgrowth of muscles. How would such a pig be achieved using CRISPR? Why would it not considered the GMO?
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Question:
Genetically modified animal that might be approved for human consumption is a super “muscly” pig made by the inactivation of the myostatin gene. During normal development, the myostatin protein prevents the overgrowth of muscles.
- How would such a pig be achieved using CRISPR?
- Why would it not considered the GMO?
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- Transforming an Animal In order to create the transgenic cow, your lab first needs to create a DNA vector containing the insulin gene. This step involves a considerable amount of scientific terminology. Make sure you understand the meaning of key terms. Match the following terms with their correct definitions. | ampicillin resistance gene 5 restriction site 6 Origin of replication 7 Ligase 2 promoter 3 Xhol Ч ехоn is a region of DNA that is not transcribed. is the location in the plasmid that is recognized by the restriction enzyme Xhol. is an enzyme that joins DNA fragments together. is the location on the plasmid where DNA replication begins. is a region of DNA that initiates transcription of a gene. is an restriction enzyme that looks for the sequence TCGA. is a gene that enables you to identify bacterial cells that have taken up the plasmid.Complete the protein synthesis for the partial DNA sequence for a normal FGFR3 gene (TOP) and mutated FGFR3 gene (BOTTOM). Remember, when filling in mRNA, use capital letters only. When filling in amino acids, use three letters, with the first letter capitalized. If you do not use this format, your answer may be marked wrong. DNA CCG TTC GGG GAA ССС MRNA Amino Acid DNA CCG TTC GGG GAA TCC MRNA Amino Acida gm animal that may be approved for human consumption by the time this book is published is a super muscly pig made by inactivation of the myostatin gene. During normal development, myostatin protein prevents the overgrowth of muscles. (Given that effective deletions can be made in the genome by using CRISPR/Cas9. It targets deletions of varied length. By this technique, the transcriptional status of the target gene is not affected. The gene deletions produced by CRISPR/Cas9 lead to the production of the correct type of junctions at very high frequencies), how could the super muscly pig have been generated? Answer the questions in the photo as well. ( i included my answer for 22a in the question above, if you believe you have a better answer by all means include that as well)
- The prospect of using gene therapy to alleviate genetic conditions is still a vision of the future. Gene therapy for adenosine deaminase deficiency has proved to be quite promising, but many obstacles remain to be overcome. Currently, the correction of human genetic defects is done using retroviruses as vectors. For this purpose, viral genes are removed from the retroviral genome, creating a vector capable of transferring human structural genes into sites on human chromosomes within target-tissue cells. Do you see any potential problems with inserting pieces of a retroviral genome into humans? If so, are there ways to combat or prevent these problems?TOPIC: PCR and Gene Cloning Basics Question: What are 2 possible roles of CaCl2 in the transformation process?Genomic DNA from one of the seven neomycin resistant clones, AB1, that produced a PCR product with primers A/B was further examined by PCR alongside genomic DNA from neomycin resistant fibroblasts that did not generate a PCR product with primers A and B and untransformed porcine fibroblast cells. Clearly and neatly diagram an image of the agarose gel
- Complete the following problems. Restriction enzymes (REs), which cut D NA at specific sequences, are classic tools in molecular biology. Because of their specificity in cutting DNA, REs can be used to "map" DNA sequences by analyzing the fragments generated upon restriction digest, as in the example shown in Figure 1. Your task is to study the circular plasmid, pMBBS, through restriction digests. You subjected the pMBBS plasmid to complete digestion by different combinations of three REs (EcoRI, BamHI, and Xhol), and analyzed the results on an agarose gel, shown below. Using the data you can glean from this gel, answer the questions that follow. EcoRI BamHI Xhol *The same total amount of DNA was loaded in each lane. 1. What is the total size of the pMBBS plasmid in bp? Answer: bp + DNA size ladder 2. How many cut sites on the PMBBS plasmid does each RE have? EcoRI: BamHI: Xhol: 3000 bp 2500 bp -2000 bp -1500 bp 1200 bp 1000 bp 900 bp 800 bp 700 bp 600 bp 500 bp 400 bp 300 bp 200 bp…Primers designing for epitope tagging: Design forward and reverse primers to amplify the following gene with 6×HIS-tag on the N-terminus of the protein. To be cleaved and inserted into the plasmid, add restriction sites for EcoRI and HindIII at 5' and 3'. ATGCTCTCCGCCCTCGCCCGGCCTGTCAGCGCTGCTCTCCGCCGCAGCTTCAGCACCTCAGCC CAGAACAATGCTAAAGTAGCTGTGCTAGGGGCCTCTGGAGGCATCGGGCAGCCACTTTCAC TTCTCCTGAAGAACAGCCCCTTGGTGAGCCGCCTGACCCTCTATGATATCGCGCACACACCC GGAGTGGCCGCAGATCTGAGCCACATCGAGACCAAAGCCGCTGTGAAAGGCTACCTCGGAC CTGAACAGCTGCCTGACTGCCTGAAAGGTTGTGATGTGTAACell and Molecular Biology Subject: 1. Explain how the actions of the lac operon are different from the trp operon. 2. Why is it important for a multicellular organism to be able to turn on only some of its genes in any particular tissue or cell type?
- Restriction endonucleases (RE) are one of the most used enzymes in biotechnology for recombinant DNA experimentations. These enzymes consist of three major classes; I, II and III. (i) Why is RE Class II commonly utilized in the labs as compared to Class I and III? (ii) Why is RE referred as Restriction Endonuclease and not Restriction Exonuclease? (iii) Some REs are known as isoschizomers. Describe isoschizomers with an example.Please answer fast If the plasmid Lac operon has a mutated lacO operator gene that prevents the repressor from binding, which of the following will occur when lactose is absent? No beta-lactamase will be produced. A functional beta-lactamase will be produced. A non-functional beta-lactamase will be produced. Both a functional and a non-functional beta-lactamase will be produced. If the plasmid Lac operon has a mutated promotor that prevents RNA polymerase from binding, which of the following will occur when lactose is present? No beta-lactamase will be produced. A functional beta-lactamase will be produced. A non-functional beta-lactamase will be produced. Both a functional and a non-functional beta-lactamase will be produced.Instructions: After reading the Khan academy article on the Lac operon, suppose that E. coli sustains a mutation in its gene for the lac operon repressor making the repressor unable to bind to the operator. How would this mutation affect the bacterium's ability to catabolize lactose? Would the mutant strain have an advantage over the wild-type strain?