Match the following terms with their definitions and label each  component of the PCR mixture in the diagram (use the letters A-D): I. DNA polymerase II. Primers III. Nucleotides IV. Genomic DNA template A. DNA that contains the target sequence that will be replicated using PCR. B. An enzyme that copies the DNA sequence. C. A mixture of 4 nucleotides (A,G,C, and T) that will be polymerized into the replicated  DNA sequence. D. A short DNA sequence that allows the enzyme to bind and initiate polymerization.

You isolate bacterial DNA from an unknown species, and use a PCR primer that amplifies DNA only from the bacterial genus Staphylococcus. After PCR and gel electrophoresis, you do not see any bands of DNA on your gel.What does this mean?

You are performing a PCR reaction but unbeknownst to you, there is a significant pool of dUTP in the nucleotide mix (along with dCTP, dTTP, dATP, and dGTP). How might this affect your PCR product? a. If the PCR product was ligated into a plasmid and put into a cell, a totally different mRNA would be made from the insert compared to an insert made with T's.  b. If the pool of dTTP ran out before the pool of dUTP, DNA replication could no longer occur.  c. During the reaction, uracils incorporated into the product would cause the PCR product to degrade as it is being made.  d. Uracil would be incorporated into the product and would lessen the affinity of any DNA binding proteins that might bind to the product in subsequent experiments. e. Nothing would happen since polymerases can't use dUTP to make DNA.

The diagram illustrating the polymerase chain reaction (PCR) technique is provided below. How does the number of copies of the DNA region being amplified change at the end of each cycle of the polymerase chain reaction?   Group of answer choices a. The number of copies triples (or triplicates). b. The number of copies does not change. c. The number of copies quadruples (or quadruplicates). d. The number of copies doubles (or duplicates). e. The number of copies halves.

When sticky ends of restriction fragments are paired, they can be joined by Select one: a. restriction enzymes b. DNA polymerase c. RNA polymerase d. DNA ligase

PCR is a molecular biology technique where template DNA is amplified using a primer and oligonucleotides. The reaction is catalyzed by a thermostable DNA polymerase and in a particular reaction, the template strands are denatured at 95˚C. For strand hybridization, the melting temperature is 55˚C. What do you predict about the average duration of H bonds at the high temperature in comparison to the low temperature?