In 1996, a group of scientists from Scotland used a somatic cell of an adult sheep and fused it with an egg. They wanted to produce an identical cony of that sheep. The process through which a genetically identical cell or organism is produced is known as O A. cloning O B. carbon copying O C. xeroxing O D. vector analysis

1. produces multiple identical copies of a gene               [ Choose ]            a. DNA ligase              b. gene cloning            c. gel electrophoresis              d.restriction enzymes

Part 1: Matching Match the term with its corresponding definition.   a. Insertion e. Nonsense Mutation b. Deletion f. Missense Mutation c. Frameshift Mutation g. Germ-line Cells d. Silent Mutation h. Somatic Cells     ____       1.            Remove a base or bases   ____       2.            Add a base or bases   ____       3.            Mutations in these cells are not passed to future generations but are passed to other body cells derived from them   ____       4.            Mutation in which an amino acid is changed to a stop codon and the protein is truncated   ____       5.            Mutations in these cells are passed to future generations   ____       6.            Results if an insertion or deletion throws the reading frame of the gene message out of register   ____       7.            Mutation where the protein does not change   ____       8.            Mutation where a codon is changed resulting in another amino acid inserted in…

How would you go about cloning this amplified DNA into pL4440？Using your knowledge of cloning describe 5 important aspects of the method in details

Imagine you go to the doctor because you are sick. You get antibiotics, but you hurry out of the hospital as fast as you can so you don’t pick up any antibiotic resistance genes as well.  If genes were transferred directly from the environment, you could pick up a resistance gene by breathing the air.  What type of gene transfer allows cells to pick up genes directly from the environment?       a.   Transformation       b.   Transduction       c.  Conjugation       d.   All of the above

Do you feel CRISPR or other technologies are appropriate for use in human embryo’s?  Why or why not?  In what situations might this be acceptable to you?

8) The diagram to the right shows some of the DNA in a bacterium into which a human gene, X, has been successfully inserted. The bacteria that results from reproduction of this cell will most likely have the ability to DNA of bacterium Cell wall of bacterium replicate all of the genetic instructions found in humans produce vaccines to be used to immunize humans produce a human blood cells according to instruction in gene X O produce the human protein coded for by gene X

Place these events in the order they occur during mutagenesis: 1 1. The cell makes a repair error t 2. Damage to DNA by a mutagen 3. DNA repair pathways are used 1 4. A permanent change to the DNA sequence is made 1 5. Genetic variation is created in the population 1 6. A new allele is born!