Let’s say, you want to deliver a gene into a cell and in your lab, there are lot of options available to do that. Describe five different options that you can use to deliver that gene.
Q: Which goals of the Human Genome Project do you think are themost important? Why? Discuss the types…
A: The genome of an organism is defined as the whole heredity information encoded in the genetic…
Q: Discuss three important advances that have resulted from gene cloning.
A: Gene cloning or DNA cloning is a technique of molecular biology that is used to produces many…
Q: What ethical concerns might you have about this kind of genetic medicine?
A: Genetic medicine involves nucleic acids that are delivered into the body of an individual as a…
Q: Give a schematic diagram of how we can Treatment Hemophilia by using gene therapy?
A: Hemophilia is a sex linked genetic disorder that is characterized by inability to make adequate…
Q: How could the information from sequencing your genome be used against you theoretically if it fell…
A: Genome sequencing is the most powerful key to get the entire information regarding the specimen. It…
Q: Why can’t all genetic diseases be treated with gene therapy ? Explain how the ideal procedure for…
A: Gene therapy is one of the treatment methods to treat genetic diseases. It involves the replacement…
Q: What are some of the ethical concerns arising out of the information produced by the Human Genome…
A: Genotype is the genetic makeup of an organism. Human Genome Project includes the sequencing and…
Q: If a pair of 10-mer primers are used in a PCR, what would be the number of expected binding sites in…
A: PCR is a polymerase chain reaction, in this process DNA is amplified . For this amplification,…
Q: Is it safe to manipulate the genes of an organism?
A: Selective breeding is less efficient as the chances of obtaining a desired trait is more complicated…
Q: The first attempts at gene therapy began in 1990 with the treatment of a young girl with a genetic…
A: SCID stands for Severe combined Immunodeficiency. SCID is a group of very rare, life-threatening…
Q: What are some of the problems that might arise if you were trying to produce a eukaryotic protein in…
A: To describe: The problems that might arise if you were trying to produce a eukaryotic protein in a…
Q: Why is targeting a medication like BiDil at African-Americans a flawed idea from an evolutionary…
A: The African Americans patients with congestive heart failures and issues were being prescribed with…
Q: How does gene cloning produce many copies of a gene?
A: Gene is a functional unit of heredity. A gene is a sequence of nucleotides in genome that codes for…
Q: Suppose researchers learn that a particular congenital disease is caused by synthesis of a protein…
A: Recombinant DNA technology brings the the gene from multiple sources together and place it in the…
Q: Forty years after its development, the use of recombinant DNA technology is widespread and is found…
A: The technique that deals with the incorporation of a foreign or desired DNA fragment into another…
Q: CRISPR techniques allow scientists to modify specific genes while sparing all others, thus…
A: Genetic diseases are the hereditary diseases that are very difficult to treat. With the advancement…
Q: What is the Pros and Cons of Cloning?
A: Answer Cloning is the brst way to increase the product in more number bearing the same generic…
Q: You have isolated a transposable element from the human genome and have determined its DNA…
A: Transposable element also known as jumping gene, is a DNA sequence which can change its position in…
Q: What would be an advantage of using HaeIII for a cloning experiment? What would be a disadvantage?
A: The restriction enzymes or restriction endonucleases are used to cleave the DNA sequence into…
Q: Besides gene expression, what else can be assayedusing gene chips?
A: DNA microassays or DNA chips contain thousands of DNA sequences of individuals arrayed at a great…
Q: . In selecting target cells to receive a transferred gene in gene therapy in an adult person, what…
A: To cure some of the human diseases gene therapy can be given to the patient. Gene therapy is the…
Q: If you want to multiply the number of a target gene, what process you will do? Explain the process…
A: Target DNA sequence is the specific sequence which consist of nucleotides, which are mainly Adenine,…
Q: An unapproved form of gene therapy, known as enhancement gene therapy, can create considerable…
A: Answer: Introduction: Present gene therapy research has fixated on treating persons by directing the…
Q: What does whole exome sequencing test for?
A: Exomes are part of the genome, which contains only the coding portions of the genes that is the…
Q: In chapter 19 of Watson et al., Box 19-2 explains the ChIP-Chip and ChIP-Seq techniques. Explain…
A: Transcription Factors (TF) are proteins that binds to specific sequences near a structural gene and…
Q: For what the genetic engineering has been used for?
A: Genetic engineering is the direct manipulation of an organism's genes with the aid of biotechnology.…
Q: You just graduated from college and started working at a biotech startup called Scrofabulous. Your…
A: Gene is cloned with the help of polymerase chain reaction(PCR). PCR is a rapid in vitro method to…
Q: Give the scope of the following fields in Applied Genetics: Instruction: No plagiarism. Answer…
A: Applied Genetics of the white field which main change the pattern of gene expression. Thereby this…
Q: How many transposons are in the human genome?
A: Transposons or transposable elements (TE) are the DNA (deoxyribonucleic acid) sequences that can…
Q: What advantages do cDNA libraries provide over genomic DNA libraries? Describe cloning applications…
A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…
Q: are using the restriction enzyme HAEIII to digest different samples of the taster gene isolated from…
A: In gel electrophoresis DNA to be tested is broken down by various restriction endonucleases into…
Q: Why is the genome contained within a membrane?
A: A genome is a complete set of "genetic information" in an organism. It stores all of the information…
Q: A person with a rare genetic disease has a sample of her chromosomessubjected to in situ…
A: The word insitu derived from the Latin word that means in place. This suggests that the…
Q: In your opinion, what is the most compelling reason to upload transcriptome data to free, public…
A: Transcriptome means a series of mRNA or messenger RNA, molecules expressed in an organism. The human…
Q: What are the types of transposons? Please explain in detail how transposons contribute to genome…
A: Some mobile genomic components have been named as mobile genetic elements or transposable elements…
Q: In contrast with the genomic manipulations of animals and plants described in this chapter, human…
A: Genetics is a branch of science that deals in the study of genes, heredity, and genetic variation of…
Q: In a microarray experiment, if you label cDNA from Tissue #1 with a red color and cDNA from tissue…
A: Microarray is the laboratory tool which is used to detect the expression levels of hundreds of genes…
Q: The following image depicts a short stretch of sequence associated with a gene. Which of the…
A: The given DNA strand is as follow 5'- AACTATAAGCCTTGGCCGATTCGATGCTAAATTGCGCATAA-3 We know DNA has…
Q: what are Several Limitations of positional cloning?
A: Positional cloning is one of the laboratory technique of gene identification. In this method, gene…
Q: You are studying a new gene “X” that you think controls skin color in Bearded Dragons. In order to…
A: PCR stands for polymerase chain reaction. It is responsible for amplifying the specific region of…
Q: ou are studying a new gene “X” that you think controls skin color in Bearded Dragons. In order to…
A: This is a question about gene amplification.
Q: What is a gene gun used for?
A: Step 1 A gene gun or a biolistic particle delivery system is a device that can transform almost any…
Q: In your own words, describe the series of steps necessary to clone a gene.
A: Cloning is the process of producing genetically identical copies of an individual by natural or…
Q: Suppose we take tissue samples from the kidney and the liver of a healthy individual. If we compare…
A: Genetic sequences or the genome of a cell is present in the nucleus and includes DNA molecule…
Q: What is the difference between manipulating a gene for gene therapy and for producing a transgenic…
A: The utilization of artificial tools and techniques to alter the genetic material of cells or…
Let’s say, you want to deliver a gene into a cell and in your lab, there are lot of options available to do that. Describe five different options that you can use to deliver that gene.
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- I believe that there are many good things that can come out of people getting to design their baby’s genetic material. But there are also many bad things as well. From the article by Bio medical about the pros and cons of having a designer baby it states that a pro is that this type of engineering can “ might help prevent genetic diseases such as Alzheimer’s, Huntington’s Disease, down syndrome, Spinal Muscular Atrophy, and many others”. I think that it is great that we could get rid of Alzheimer’s due to how destructive it can be to the people that suffer it. But I think the other diseases that it can eliminate is horrible due to them making our world a more unique place such as people with autism, Down syndrome. By doing this it could eliminate the whole population of people with disabilities community and make everyone “normal”. Another bad that I found in the article Ethics of designer babies which states that a major flaw for these babies is “designer baby technologies suggest…You are engineering a new vector that contains a screenable marker that can be used for blue/white screening of successful clones. For each site (1, 2, and 3) on the cloning vector below, describe why it would or would not be a good place for you to put the polylinker to facilitate blue/white screening. You can assume that the polylinker itself will not interfere with coding sequence in that region. In other words, the polylinker length will be a multiple of 3 nucleotides, will not contain a stop codon, and any amino acids translated will not affect the activity of the protein in that region. The arrows indicate the direction of transcription for the gene. Note from student:As stated in the problem... "YOU CAN ASSUME THAT THE POLYLINKER ITSELF WILL NOT INTERFERE WITH CODING SEQUENCE IN THAT REGION. IN OTHER WORDS, THE POLYLINKER LENGTH WILL BE A MULTIPLE OF 3 NUCLEOTIDES, WILL NOT CONTAIN A STOP CODON, AND ANY AMINO ACIDS TRANSLATED WILL NOT AFFECT THE ACTIVITY OF THE PROTEIN IN THAT…It has been suggested that it would make the study of human diseases easier if cloned transgenic animals were produced that carried faulty versions of human genes (e.g., the gene that causes cystic fibrosis). a. Why would such animals be useful in medical research? : b. What ethical questions are raised by the creation of such transgenic animals?
- If you want to multiply the number of a target gene, what process you will do? Explain the process in detailYou are engineering a new vector that contains a screenable marker that can be used for blue/white screening of successful clones. For each site (1, 2, and 3) on the cloning vector below, describe why it would or would not be a good place for you to put the polylinker to facilitate blue/white screening. You can assume that the polylinker itself will not interfere with coding sequence in that region. In other words, the polylinker length will be a multiple of 3 nucleotides, will not contain a stop codon, and any amino acids translated will not affect the activity of the protein in that region. The arrows indicate the direction of transcription for the gene.There is a hypothetical gene related to the nervous system of Drosophila. Describe all the methods, steps, and key substances you need to obtain to use the following techniques in experimental design to study the gene: - In situ hybridization (to find the mRNA) - Immunohistochemistry (to find the protein) - CRISPR-Cas9 (for loss of function) - Expression vector (for gain of function)
- You just graduated from college and started working at a biotech startup called Scrofabulous. Your first job assignment is to clone the pig gene for the hormone prolactin. Assume that the pig gene for prolactin has not yet been isolated, sequenced, or mapped; What would be the most useful and economical first step to go about identifying and cloning the pig gene for prolactin? use the amino acid sequence of mouse prolactin to design a pair of degenerate oligonucleotide PCR primers to PCR-amplify the pig prolactin gene. RNAseq the pituitary gland of the pig, the most abundant gene is likely to to be prolactin Conduct a proteome search for peptides that match parts of mouse prolactin protein Sequence the pig genome, then translate the genome to find the gene predicted to encode for prolactin Crystalize the mouse prolactin protein and use Google's DeepMind Al to find the closest amino acid sequence in the pig proteomeEach of the following describes a distinctive step in a genomic technology or experimental design. Match the name of the technology or experimental design to the description. Answers may be used more than once or not at all. Add fluorescent tags onto the single-stranded sample of nucleic acids before the sample is applied to a glass slide. [ Choose ]When aligned to a reference genome, read depth indicates duplications and deletions. [ Choose ]A spot appears as a mix of two fluorescent colors if the individual is heterozygous. [ Choose ]An experimental design that relies on identifying unrelated individuals that have one of two phenotypes, then looking for a correlation between individual phenotype and genotype. [ Choose ] choices: GWAS, RNA microarray, RNA sequencing, DNA microarray, quantitative genetics, family study, genomic resequencingYou’ve made your construct and placed it into E. coli! Congratulations, you have made a transgenic organism. Your investors will want to know about quality control. How will you check that the correct piece of DNA is in your vector? How will you check to make sure the gene is transcribed? How will you check to make sure that the GasP protein is made in E. coli? Your investors are concerned that the GasP protein might not be sufficiently produced under normal laboratory conditions. They suggest controlling the transcription of the gasP gene using a chemical that will “trigger” its transcription. What type of promoter could be used? What chemical will you use to control transcription? How does this method of control work?
- You’ve made your construct and placed it into E. coli! Congratulations, you have made a transgenic organism. Your investors will want to know about quality control. How will you check that the correct piece of DNA is in your vector? How will you check to make sure the gene is transcribed? How will you check to make sure that the GasP protein is made in E. coli?Explain why exome sequencing can be almost as valuable as genome sequencing. (Explain in your own words)You are asked to sequence a piece of DNA to determine if it is from a gene thought to be involved in the development of breast cancer. The sequence of the template strand is ATGCCCGTAATCGTTA and you are given the primer TAACGA. You take these along with a sequencing kit that contains everything else needed for sequencing. You then run the sequencing experiment and analyze the results on a sequencing gel. Which of the following gels (A-D) is the correct sequencing gel for this experiment? Answers: A-D A A BB CC DD Question #3 attachment A ATOC B с A TO C ATOC ATOC