Place the steps in the photo in order during DNA extraction. Place the following in the correct order in terms of steps taken, followed by the consideration appropriate to each of the different steps (soStep/Consideration/Step/Consideration etc)E Step: Precipitation of nucleic acidsI Step: Disruption of the cells to release their contents (homogenisation)3.E Considerations: methods used to enhance yield of final extract include keeping the solution cold4I Considerations: is the tissue bacterial, fungal, plant or animal, thus with or without a cell wall?# Considerations: add enzymes to assist in the degradation of proteins, and also RNAE Step: Removal of non-nucleic acid components, such as protein, lipids5n

DNA extraction consists of three simple steps: lysis, precipitation, and purification.

Place the following in the correct order in terms of steps taken, followed by the consideration appropriate to each of the different steps (so Step/Consideration/Step/Consideration etc) I Step: Precipitation of nucleic acids 2 I Step: Disruption of the cells to release their contents (homogenisation) I Considerations: methods used to enhance yield of final extract include keeping the solution cold 4. I Considerations: is the tissue bacterial, fungal, plant or animal, thus with or without a cell wall? | Considerations: add enzymes to assist in the degradation of protelns, and also RNA 6. I Step: Removal of non-nucleic acid components, such as protein, lipids

Place the following in the correct order in terms of steps taken, followed by the consideration appropriate to each of the different steps (so Step/Consideration/Step/Consideration etc) I Step: Precipitation of nucleic acids 2 I Step: Disruption of the cells to release their contents (homogenisation) I Considerations: methods used to enhance yield of final extract include keeping the solution cold 4. I Considerations: is the tissue bacterial, fungal, plant or animal, thus with or without a cell wall? | Considerations: add enzymes to assist in the degradation of protelns, and also RNA 6. I Step: Removal of non-nucleic acid components, such as protein, lipids

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter18: Instrumentation In The Operating Room

Section: Chapter Questions

Problem 3CS

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Place the following in the correct order in terms of steps taken, followed by the consideration appropriate to each of the different steps (so Step/Consideration/Step/Consideration etc) 1 | Considerations: add enzymes to assist in the degradation of proteins, and also RNA Considerations: methods used to enhance yield of final extract include keeping the solution cold 3 | Step: Removal of non-nucleic acid components, such as protein, lipids 4 | Considerations: is the tissue bacterial, fungal, plant or animal, thus with or without a cell wall? Step: Precipitation of nucleic acids 6 | Step: Disruption of the cells to release their contents (homogenisation)
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