For Pet41 (choose Pet41 a, b, or c as provided in the image) how would you design the primers (forward and reverse) for the following gene of interest and what restriction enzymes would be used (as shown in the image)? Be sure to explain and elaborate on why selected and how. Gene of Interest: atgggc gacaaaggga 241 cccgagtgtt caagaaggcc agtccaaatg gaaagctcac cgtctacctg ggaaagcggg 301 actttgtgga ccacatcgac ctcgtggacc ctgtggatgg tgtggtcctg gtggatcctg 361 agtatctcaa agagcggaga gtctatgtga cgctgacctg cgccttccgc tatggccggg 421 aggacctgga tgtcctgggc ctgacctttc gcaaggacct gtttgtggcc aacgtacagt 481 cgttcccacc ggcccccgag gacaagaagc ccctgacgcg gctgcaggaa cgcctcatca 541 agaagctggg cgagcacgct taccctttca cctttgagat ccctccaaac cttccatgtt 601 ctgtgacact gcagccgggg cccgaagaca cggggaaggc ttgcggtgtg gactatgaag 661 tcaaagcctt ctgcgcggag aatttggagg agaagatcca caagcggaat tctgtgcgtc 721 tggtcatccg gaaggttcag tatgccccag agaggcctgg cccccagccc acagccgaga 781 ccaccaggca gttcctcatg tcggacaagc ccttgcacct agaagcctct ctggataagg 841 agatctatta ccatggagaa cccatcagcg tcaacgtcca cgtcaccaac aacaccaaca 901 agacggtgaa gaagatcaag atctcagtgc gccagtatgc agacatctgc cttttcaaca 961 cagctcagta caagtgccct gttgccatgg aagaggctga tgacactgtg gcacccagct 1021 cgacgttctg caaggtctac acactgaccc ccttcctagc caataaccga gagaagcggg 1081 gcctcgcctt ggacgggaag ctcaagcacg aagacacgaa cttggcctct agcaccctgt 1141 tgagggaagg tgccaaccgt gagatcctgg ggatcattgt ttcctacaaa gtgaaagtga 1201 agctggtggt gtctcggggc ggcctgttgg gagatcttgc atccagcgac gtggccgtgg 1261 aactgccctt caccctaatg caccccaagc ccaaagagga acccccgcat cgggaagttc 1321 cagagaacga gacgccagta gataccaatc tcatagaact tgacacaaat gatgacgaca 1381 ttgtatttga ggactttgct cgccagagac tgaaaggcat gaaggatgac aaggaggaag 1441 aggaggatgg taccggctct ccacagctca acaacagata g Note from student: I believe Restriction Enzymes should not cut the gene of interest otherwise would not function.
For Pet41 (choose Pet41 a, b, or c as provided in the image) how would you design the primers (forward and reverse) for the following gene of interest and what restriction enzymes would be used (as shown in the image)? Be sure to explain and elaborate on why selected and how.
Gene of Interest:
atgggc gacaaaggga 241 cccgagtgtt caagaaggcc agtccaaatg gaaagctcac cgtctacctg ggaaagcggg 301 actttgtgga ccacatcgac ctcgtggacc ctgtggatgg tgtggtcctg gtggatcctg 361 agtatctcaa agagcggaga gtctatgtga cgctgacctg cgccttccgc tatggccggg 421 aggacctgga tgtcctgggc ctgacctttc gcaaggacct gtttgtggcc aacgtacagt 481 cgttcccacc ggcccccgag gacaagaagc ccctgacgcg gctgcaggaa cgcctcatca 541 agaagctggg cgagcacgct taccctttca cctttgagat ccctccaaac cttccatgtt 601 ctgtgacact gcagccgggg cccgaagaca cggggaaggc ttgcggtgtg gactatgaag 661 tcaaagcctt ctgcgcggag aatttggagg agaagatcca caagcggaat tctgtgcgtc 721 tggtcatccg gaaggttcag tatgccccag agaggcctgg cccccagccc acagccgaga 781 ccaccaggca gttcctcatg tcggacaagc ccttgcacct agaagcctct ctggataagg 841 agatctatta ccatggagaa cccatcagcg tcaacgtcca cgtcaccaac aacaccaaca 901 agacggtgaa gaagatcaag atctcagtgc gccagtatgc agacatctgc cttttcaaca 961 cagctcagta caagtgccct gttgccatgg aagaggctga tgacactgtg gcacccagct 1021 cgacgttctg caaggtctac acactgaccc ccttcctagc caataaccga gagaagcggg 1081 gcctcgcctt ggacgggaag ctcaagcacg aagacacgaa cttggcctct agcaccctgt 1141 tgagggaagg tgccaaccgt gagatcctgg ggatcattgt ttcctacaaa gtgaaagtga 1201 agctggtggt gtctcggggc ggcctgttgg gagatcttgc atccagcgac gtggccgtgg 1261 aactgccctt caccctaatg caccccaagc ccaaagagga acccccgcat cgggaagttc 1321 cagagaacga gacgccagta gataccaatc tcatagaact tgacacaaat gatgacgaca 1381 ttgtatttga ggactttgct cgccagagac tgaaaggcat gaaggatgac aaggaggaag 1441 aggaggatgg taccggctct ccacagctca acaacagata g
Note from student: I believe Restriction Enzymes should not cut the gene of interest otherwise would not function.
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