Genetics: Analysis and Principles
Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 18.3, Problem 2COMQ

The cl gene that encodes the λ repressor has two promoters designated P RE and   P RM . Which of the following statements is false?

a. P RE is activated by the cII protein.

b. P RE is activated by the λ repressor.

c. P RM is turned on after P RE .

d. P RM is needed to maintain the lysogenic cycle.

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E. coli has five genes that code for enzymes that make tryptophan. These genes are regulated by a single promoter and transcribed as one long gene. The presence of tryptophan shut down the production of tryptophan by the cell by binding to the repressor. This changes the repressors shape allowing it to bind to DNA operator, blocking RNA polymerase and cutting off the production of tryptophan. a. Describe what woud happen to the operon if some of the cells had a mutation on the repressor, not allowing it to bind with tryptophan. The repressor is described as an allosteric protein. What does this mean? b. Does the tryptophan model demonstrate an inducible or repressible operon? What is your evidence? *
E. coli are grown on a medium containing lactose. Once glucose is added to the medium, the bacteria stop fermenting lactose. Which of the following BEST explains the observed effect? A. Cellular levels of cAMP are lowB.Glucose is bound to the promoter C. Repressor protein is bound to the operator D. Repressor protein is bound to the promoter
E. coli has five genes that code for enzymes that make tryptophan. These genes are regulated by a single promoter and transcribed as one long gene. The presence of tryptophan shut down the production of tryptophan by the cell by binding to the repressor. This changes the repressors shape allowing it to bind to DNA operator, blocking RNA polymerase and cutting off the production of tryptophan. a. Describe what would happen to the operon if some of the cells had a mutation on the repressor, not allowing it to bind with tryptophan. The repressor is described as an allosteric protein. What does this mean? b. Does the tryptophan model demonstrate an inducible or repressible operon? What is your evidence?*

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Genetics: Analysis and Principles

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