Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 18, Problem 7EQ
A researcher identified a mutation in
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This question combines your knowledge of bacterial conjugationand the genetic regulation that directs thephage λ reproductive cycles. When researchers mix donor Hfr strains with recipient F– bacteria that are lysogenic for phage λ, the conjugated cells survive normally. However, if donor Hfr strains that are lysogenic for phage λ conjugate with recipient F– bacteria that do not contain any phage λ, the recipient cells often lyse, due to the induction of λ into the lytic cycle. Based on your knowledge of the regulation of the two reproductive cycles of phage λ, explain this experimental observation.
The figure above shows a schematic of genes and transcription control elements from phage λ. Use this figure as an aid to help you describe the molecular events involved in:
a) The establishment of lysogeny
b) The establishment of a lytic life cycle
Certain environmental conditions such as exposure to UV light areknown to activate lysogenic λ prophages and cause them to progressinto the lytic cycle. UV light initially causes the repressor protein to be proteolytically degraded. Make a flow diagram showing the subsequent events that lead to the lytic cycle. (Note: The xis gene codes for an enzyme that is necessary to excise the λ prophage from the E. coli chromosome. The enzyme integrase is also necessary for this excision.)
Chapter 18 Solutions
Genetics: Analysis and Principles
Ch. 18.1 - 1. What is a common feature found in all viruses?...Ch. 18.1 - 2. Viral genomes can be
a. DNA or RNA.
b....Ch. 18.2 - Prob. 1COMQCh. 18.2 - Prob. 2COMQCh. 18.3 - A mutation in phage results in 10-fold greater...Ch. 18.3 - 2. The cl gene that encodes the λ repressor has...Ch. 18.4 - A viral protein that is needed to make HIV DNA is...Ch. 18.4 - Prob. 2COMQCh. 18.4 - After HIV components are made, what is the correct...Ch. 18 - 1. Discuss why viruses are considered nonliving.
Ch. 18 - What structural features are common to all...Ch. 18 - 3. What are the similarities and differences among...Ch. 18 - Prob. 4CONQCh. 18 - Prob. 5CONQCh. 18 - Prob. 6CONQCh. 18 - Prob. 7CONQCh. 18 - Prob. 8CONQCh. 18 - Prob. 9CONQCh. 18 - Prob. 10CONQCh. 18 - 11. What is a prophage, a provirus, and an...Ch. 18 - Prob. 12CONQCh. 18 - Prob. 13CONQCh. 18 - 14. With regard to promoting the lytic or...Ch. 18 - 15. How do therepressor and the cro protein affect...Ch. 18 - Prob. 16CONQCh. 18 - Figure 18.11 shows a genetic switch that controls...Ch. 18 - Prob. 18CONQCh. 18 - Prob. 19CONQCh. 18 - Explain the role of RNase H (a component of...Ch. 18 - Prob. 21CONQCh. 18 - Prob. 22CONQCh. 18 - Prob. 23CONQCh. 18 - 24. Compare and contrast the roles of fully...Ch. 18 - 25. Describe the role of the Gag polyprotein...Ch. 18 - Prob. 26CONQCh. 18 - Prob. 27CONQCh. 18 - 1. Discuss how researchers determined that TMV is...Ch. 18 - Prob. 2EQCh. 18 - What is a reconstituted virus?Ch. 18 - Following the infection of healthy tobacco leaves...Ch. 18 - Prob. 5EQCh. 18 - Prob. 6EQCh. 18 - A researcher identified a mutation in PR of phage ...Ch. 18 - Experimentally, when an E. coli bacterium already...Ch. 18 - 9. A bacterium is exposed to a drug that inhibits...Ch. 18 - This question combines your knowledge of bacterial...Ch. 18 - Prob. 1QSDCCh. 18 - 2. Certain environmental conditions such as...Ch. 18 - 3. Browse the Internet to determine the drugs that...
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- When various strains of λ phage are seeded on a lawn of E. coli, they can form clear or turbid plaques. (b) For mutant λ phages that can only form clear plaques, give two different types of mutation in the phage that can explain the clear plaque phenotype.arrow_forwardBacteriophage lambda is integrated in DNA of a host cell, which is exposed to UV light in a body of water in the environment. Describe the sequence of events that occurs in its life cycle. Briefly describe the role of the Lambda repressor (cI) and cII activator to regulate the switch between the lysogenic and lytic cycles of phage Lambda.arrow_forwardA bacterium is exposed to a drug that inhibits the N protein. Whatwould you expect to happen if the bacterium was later infected byphage λ? Would phage λ follow the lytic cycle, the lysogenic cycle,or neither? Explain your answer.arrow_forward
- The linear dsDNA genome of λ binds on the LamB receptor of E. Coli and conducts a normal lysogenic cycle. Exposure to stress will cause the excision of λ prophage from the E. Coli genome. The excised λ genome is then replicated, packaged, and released from the cell as mature λ phage particles and ready to infect other bacterial cells. Among λ phage particles,the transducing phage mediates a specific type of recombination. Understand this scenario and answer the following questions. 1. What are the basic requirements for the insertion of λ into the E. Coli genome? 2. What special features are found in the λ insertion site? 3. What type of recombination occurs with λ insertion in the E. Coli genome? 4. How you will differentiate λ transducing phage from normal λ phage? 5. What exclusive mechanism λ phage utilizes for recombination?arrow_forwardExperimentally, when an E. coli bacterium already has a λ prophageintegrated into its chromosome, another λ phage cannot usually infectthe cell and establish the lysogenic or lytic cycle. Based on yourunderstanding of the genetic regulation of the phage λ reproductivecycles, why do you think the other phage would be unsuccessful?arrow_forwardLet’s suppose a drug inhibits the function of the N protein. Would such a drug favor the lysogenic cycle, favor the lytic cycle, or prevent both cycles from occurring?arrow_forward
- M13 is a filamentous phage that infects the bacterium Escherichia coli. Infection with M13 is not lethal. However, the infection causes turbid plaques in E. coli because infected bacteria grow slower than the surrounding uninfected bacteria. This phage has been engineered to act as a vector system. Explain how the amplification of gene of interest works in this phage with illustration.arrow_forwardFrom the perspective of a bacteriophage, what is the advantage of being able to follow either a lytic or a lysogenic cycle?arrow_forwardWhat is the difference between the lytic and lysogenic cycle of bacteriophages? In the lysogenic cycle, the phage DNA integrates itself into the host genome after penetration, and continues to biosynthesis when conditions change The host cell is not killed in the lysogenic cycle The lytic cycle occurs in the nucleus, while the lysogenic cycle occurs in the cytoplasm In the lysogenic cycle, penetration is achieved through phagocytosisarrow_forward
- Describe how the lambda phage controls the lytic and lysogenic cycles. Include cro, cI, cII, and cIII in your description. Which of these proteins is responsible for the initiation of lysogeny or lytic pathways?arrow_forwardIndicate whether each statement applies to the lytic or lysogenic cycle of phage A, or to both. 1. The host cell is lysed. (Click to select) v 2. The genetic material of the phage is copied. (Click to select) v 3. The genetic material of the phage is integrated into the bacterium's chromosome. (Click to select) v 4. Two ends of the phage DNA become covalently attached to one another. (Click to select) v 5. The integrase gene is turned on.: (Click to select) v (Click to select) lytic lysogenic botharrow_forwardThe protein of T2 bacteriophage is labeled with 35S and the DNA with 32p. Where would one detect most of the 32p if the T2 bacteriophage were allowed to infect the bacteria? O on the phage coat surrounding the T2 bacteriophage on the membrane of the infected bacteria O inside the T2 bacteriophage capsid O inside the infected bacteria O The 32P would be equally distributed within the T2 bacteriophage and infected bacteriaarrow_forward
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