Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 18, Problem 9EQ
A bacterium is exposed to a drug that inhibits the N protein. What would you expect to happen if the bacterium was later infected by phage
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Indicate whether each statement applies to the lytic or lysogenic cycle of phage A, or to both.
1. The host cell is lysed. (Click to select) v
2. The genetic material of the phage is copied. (Click to select) v
3. The genetic material of the phage is integrated into the bacterium's chromosome. (Click to select) v
4. Two ends of the phage DNA become covalently attached to one another. (Click to select) v
5. The integrase gene is turned on.: (Click to select) v
(Click to select)
lytic
lysogenic
both
If a single bacteriophage infects one E. coli cell present on a lawn of bacteria and, upon lysis, yields 185 viable viruses, how many phages will exist in a single plaque if 5 more lytic cycles occur?
Express your answer using three significant figures (example: 1.11×10^3).
The figure depicts five stages of the lytic cycle.
*********
Select the statement that describes stage 3 of the lytic cycle,
as shown in the diagram.
The host cell synthesizes cellular proteins to prepare
for lysis.
The host cell incorporates phage DNA into its
own genome.
The host cell replicates the phage DNA.
O The host cell synthesizes proteins that degrade
phage DNA.
Chapter 18 Solutions
Genetics: Analysis and Principles
Ch. 18.1 - 1. What is a common feature found in all viruses?...Ch. 18.1 - 2. Viral genomes can be
a. DNA or RNA.
b....Ch. 18.2 - Prob. 1COMQCh. 18.2 - Prob. 2COMQCh. 18.3 - A mutation in phage results in 10-fold greater...Ch. 18.3 - 2. The cl gene that encodes the λ repressor has...Ch. 18.4 - A viral protein that is needed to make HIV DNA is...Ch. 18.4 - Prob. 2COMQCh. 18.4 - After HIV components are made, what is the correct...Ch. 18 - 1. Discuss why viruses are considered nonliving.
Ch. 18 - What structural features are common to all...Ch. 18 - 3. What are the similarities and differences among...Ch. 18 - Prob. 4CONQCh. 18 - Prob. 5CONQCh. 18 - Prob. 6CONQCh. 18 - Prob. 7CONQCh. 18 - Prob. 8CONQCh. 18 - Prob. 9CONQCh. 18 - Prob. 10CONQCh. 18 - 11. What is a prophage, a provirus, and an...Ch. 18 - Prob. 12CONQCh. 18 - Prob. 13CONQCh. 18 - 14. With regard to promoting the lytic or...Ch. 18 - 15. How do therepressor and the cro protein affect...Ch. 18 - Prob. 16CONQCh. 18 - Figure 18.11 shows a genetic switch that controls...Ch. 18 - Prob. 18CONQCh. 18 - Prob. 19CONQCh. 18 - Explain the role of RNase H (a component of...Ch. 18 - Prob. 21CONQCh. 18 - Prob. 22CONQCh. 18 - Prob. 23CONQCh. 18 - 24. Compare and contrast the roles of fully...Ch. 18 - 25. Describe the role of the Gag polyprotein...Ch. 18 - Prob. 26CONQCh. 18 - Prob. 27CONQCh. 18 - 1. Discuss how researchers determined that TMV is...Ch. 18 - Prob. 2EQCh. 18 - What is a reconstituted virus?Ch. 18 - Following the infection of healthy tobacco leaves...Ch. 18 - Prob. 5EQCh. 18 - Prob. 6EQCh. 18 - A researcher identified a mutation in PR of phage ...Ch. 18 - Experimentally, when an E. coli bacterium already...Ch. 18 - 9. A bacterium is exposed to a drug that inhibits...Ch. 18 - This question combines your knowledge of bacterial...Ch. 18 - Prob. 1QSDCCh. 18 - 2. Certain environmental conditions such as...Ch. 18 - 3. Browse the Internet to determine the drugs that...
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- You are studying your favorite (hypothetical) bacteriophage named M277. It infects E. coli and stays dormant within the bacterial cell. How does a phage become dormant within a cell? Generally, describe the steps of infection by phage in this scenarioarrow_forwardIn a petri dish with solidified agar with escherichia coli, enterobacter aerogenes and staphylococcus aureus, you streak a loopfull of lytic T4-phage in a single line onto the center of the the dish, how do you know if bacteriophage infected the bacteria. why didn't the bacteriophage infect all 3 bacteria?arrow_forwardIf a single bacteriophage infects one E. coli cell present on a lawn of bacteria and, upon lysis, yields 200 viable viruses, how many phages will exist in a single plaque if three more lytic cycles occur?arrow_forward
- A researcher is studying the rII locus of phage T4. FourrII− strains are obtained: A, B, C, and D. In the first experiment, E. coli strain K(λ) is coinfected with two rII− strains simultaneously and the results are recorded. Infection with A and B phage = lysis occurs Infection with A and C phage = lysis occurs Infection with B and C phage = no lysis occurs Infection with B and D phage = no lysis occurs Infection with C and D phage = no lysis occurs In a second experiment, coinfections are performed first in E. coli strain B, then the progeny phage are used to infect E. coli strain K(λ). Progeny of A and B phage = plaques form Progeny of B and C phage = plaques form Progeny of C and D phage = plaques form Progeny of B and D phage = no plaques from Which conclusions are consistent with these data? Why? A) Strains A and B carry mutations in the same gene. B) Strains B and D both carry the same mutation. C) Strains B, C, and D carry mutations in the same gene. D)…arrow_forwardThe figure above shows a schematic of genes and transcription control elements from phage λ. Use this figure as an aid to help you describe the molecular events involved in: a) The establishment of lysogeny b) The establishment of a lytic life cyclearrow_forwardIn Hershey-Chase experiment, bacteriophages protein coats were tagged with radioactive isotope S-32. These phages were used to infect E. coli cells and the cells were further centrifuged to form pellets. Why was the radioactivity level of S-32 found greater outside the cells compared to the E. coli cell pellets? Explain briefly. If the experiment is repeated in the same manner but this time the phage protein coats are labelled with isotope X and the phage DNA with isotope Y, which isotope’s radioactivity will be found in greater amounts in the E. coli cell pellets after centrifugation? Explain briefly.arrow_forward
- When various strains of λ phage are seeded on a lawn of E. coli, they can form clear or turbid plaques. (b) For mutant λ phages that can only form clear plaques, give two different types of mutation in the phage that can explain the clear plaque phenotype.arrow_forwardA researcher identified a mutation in PR of phage λ that causes itstranscription rate to be increased 10-fold. Do you think this mutationwould favor the lytic or lysogenic cycle? Explain your answer.arrow_forwardWhy is a webbed plate capable of producing a higher lysate titer (pfu/mL) than a cleared plate? a.) A webbed plate has plaques that have gone through more rounds of replication because the number of host cells did not limit phage replication, creating exponentially more phage particles b.) A webbed plate has no more host available for replication, meaning the plate is full of exponentially higher amounts of phage c.) A cleared plate has less bacteria than a webbed plate, so there are more plaques present d.) A cleared plate contains more phage than a webbed plate because it started with a more concentrated phage samplearrow_forward
- When bacteriophage P1 causes E. coli to lyse, the resulting materialis called a P1 lysate. What type of genetic material would befound in most of the P1 phages in the lysate? What kind of geneticmaterial is occasionally found within a P1 phage?arrow_forwardphage can be "induced" as a result of DNA damage. Consequences of induction include. Temperate phage, e.g lambda phage; lytic cycle and transduction Lytic T4 phage, assembly, conjugation O Lytic phage, e.g. T4 phage; lysogenic cycle, prophage formation Temperate phage, e.g. lambada phage, prophage formation, transformation O T4 phage, lytic cycle, specialized trasnductionarrow_forwardA researcher is studying the r// locus of phage T4. Three //-- strains are obtained: A, B, and C. E. coli strain K(A) is coinfected with two rl strains simultaneously and the results are recorded. Infection with A and B phage = lysis occurs Infection with A and C phage = lysis occurs Infection with B and C phage = no lysis occurs What can be inferred from this complementation test? Multiple Choice Strains A, B, and C have mutations in three different genes. Strains A and B have mutations in the same gene, and strain C has a mutation in a different gene. Strains A and C have mutations in the same gene, and strain B has a mutation in a different gene. Strains A, B, and C have mutations in the same gene. Strains B and C have mutations in the same gene, and strain A has a mutation in a different gene.arrow_forward
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