Concept explainers
A researcher is studying the rII locus of phage T4. FourrII− strains are obtained: A, B, C, and D. In the first experiment, E. coli strain K(λ) is coinfected with two rII− strains simultaneously and the results are recorded.
Infection with A and B phage = lysis occurs
Infection with A and C phage = lysis occurs
Infection with B and C phage = no lysis occurs
Infection with B and D phage = no lysis occurs
Infection with C and D phage = no lysis occurs
In a second experiment, coinfections are performed first in E. coli strain B, then the progeny phage are used to infect E. coli strain K(λ).
Progeny of A and B phage = plaques form
Progeny of B and C phage = plaques form
Progeny of C and D phage = plaques form
Progeny of B and D phage = no plaques from
Which conclusions are consistent with these data? Why?
- A) Strains A and B carry mutations in the same gene.
- B) Strains B and D both carry the same mutation.
- C) Strains B, C, and D carry mutations in the same gene.
- D) Strain A carries a mutation in a different gene than strains B, C, and D.
- E) Strains B and C both carry the same mutation.
- F) A, B, C, and D carry mutations in the same gene.
Trending nowThis is a popular solution!
Step by stepSolved in 2 steps
- 3 2. The table below was obtained by P1 phage transduction followed by media selection of an E. coli strain lacking the three genes. Determine the map order of the genes given the information provided. Demonstrate CLEARLY which are closer or farther from each other (no numbers needed). 1 2 MAP: Experiment Selected marker Ala+ Gly+ Ser+ Unselected markers (select- ed for by subsequent plating) 27% Ser+, 2% Gly+ 3% Ser+, 1% Ala+ 35% Ala+, 4% Gly+arrow_forwardphage can be "induced" as a result of DNA damage. Consequences of induction include. Temperate phage, e.g lambda phage; lytic cycle and transduction Lytic T4 phage, assembly, conjugation O Lytic phage, e.g. T4 phage; lysogenic cycle, prophage formation Temperate phage, e.g. lambada phage, prophage formation, transformation O T4 phage, lytic cycle, specialized trasnductionarrow_forwardWhich of the following situations leads to lysogeny during phage lambda infection of E. coli? Group of answer choices 1.cI binding at PRM 2.cII binding at PL and PR 3.Cro binding at OR3 4.Digestion of CII by host proteasearrow_forward
- Discuss bacterial conjugation and the three mating types of bacteria (F-, F+, and Hfr).arrow_forwardLambda phage çan facilitate bacterial chemosomal DNA transfer through the process of specialized transduction / generalized transduction (copy and paste the correct answer) A by transferring specific bacterial DNA / bacterial DNA at random (copy and paste the correct answer) A from a donor cell / recipient cell (copy and paste the correct answer) A to a donor cell / recipient cell (copy and paste the correct answer).arrow_forwardIf a single bacteriophage infects one E. coli cell present on a lawn of bacteria and, upon lysis, yields 200 viable viruses, how many phages will exist in a single plaque if three more lytic cycles occur?arrow_forward
- Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSONBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStaxAnatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
- Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & CompanyLaboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education