Genetics: From Genes to Genomes
6th Edition
ISBN: 9781259700903
Author: Leland Hartwell Dr., Michael L. Goldberg Professor Dr., Janice Fischer, Leroy Hood Dr.
Publisher: McGraw-Hill Education
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Chapter 16, Problem 16P
For each of the following mutant E. coli strains, plot a 30-minute time course of concentration of β-galactosidase, permease, and acetylase enzymes grown under the following conditions: For the first 10 minutes, no lactose is present; at 10 minutes, lactose becomes the sole carbon source. Plot concentration on the y-axis, time on the x-axis. (Don’t worry about the exact units for each protein on the y-axis.)
| a. | I-P+o+Z+Y+A+/ I+P+o+Z-Y+A+ |
| b. | I-P+ocZ+Y+A+/ I+P+o+Z-Y+A+ |
| c. | IsP+o+Z+Y+A+/ I-P+o+Z-Y+A+ |
| d. | I-P-o+Z+Y+A+/ I-P+ocZ+Y-A+ |
| e. | I-P+o+Z-Y+A+/ I-P-ocZ+Y-A+ |
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Students have asked these similar questions
For E. coli strains with the lac genotypes show below, use a plus sign (+) to indicate the synthesis of β-galactosidase and permease and a minus sign (–) to indicate no synthesis of the proteins.
For each of the following genotypes, explain how mutation (identified by a (-) will affect the
organism grown in lactose medium. Indicate whether
a) B-galactosidase will be synthesized or not,
b) synthesis of B-galactosidase is inducible (1) or constitutive (C) and
c) growth of the organism will occur or not.
i. I+P+0-Z+Y+A+/l+P+O+Z-Y-A-
ii. I-P+0+Z+Y+A+/I+P-O+Z+Y+A+
Given the following phenotypes, explain how the mutation (identified by a (-) superscript) will affect the E. coli grown in lactose medium.
l+p+o+z-y+
i-p+o+z+y+
l+p+o-z+y+
l+p-o+z+y+
Chapter 16 Solutions
Genetics: From Genes to Genomes
Ch. 16 - For each of the terms in the left column, choose...Ch. 16 - The following statement occurs early in this...Ch. 16 - One of the main lessons of this chapter is that...Ch. 16 - All mutations that abolish function of the Rho...Ch. 16 - The figure at the beginning of this chapter shows...Ch. 16 - The promoter of an operon is the site to which RNA...Ch. 16 - You are studying an operon containing three genes...Ch. 16 - You have isolated a protein that binds to DNA in...Ch. 16 - You have isolated two different mutants reg1 and...Ch. 16 - Bacteriophage , after infecting a cell, can...
Ch. 16 - Mutants were isolated in which the constitutive...Ch. 16 - Suppose you have six strains of E. coli. One is...Ch. 16 - The previous problem raises some interesting...Ch. 16 - For each of the E. coli strains containing the lac...Ch. 16 - For each of the following growth conditions, what...Ch. 16 - For each of the following mutant E. coli strains,...Ch. 16 - Maltose utilization in E. coli requires the...Ch. 16 - Seven E. coli mutants were isolated. The activity...Ch. 16 - Cells containing missense mutations in the crp...Ch. 16 - Six strains of E.coli mutants 16 that had one of...Ch. 16 - a. The original constitutive operator mutations in...Ch. 16 - In an effort to determine the location of an...Ch. 16 - Prob. 23PCh. 16 - The footprinting experiment described in Fig....Ch. 16 - Why is the trp attenuation mechanism unique to...Ch. 16 - a. How many ribosomes are required at a minimum...Ch. 16 - The following is a sequence of the leader region...Ch. 16 - For each of the E. coli strains that follow,...Ch. 16 - Prob. 29PCh. 16 - For each element in the list that follows,...Ch. 16 - Among the structurally simplest riboswitches are...Ch. 16 - Great variation exists in the mechanisms by which...Ch. 16 - Many genes whose expression is turned on by DNA...Ch. 16 - In 2005, Frederick Blattner and his colleagues...Ch. 16 - The E.coli MalT protein is a positive regulator of...Ch. 16 - Prob. 36PCh. 16 - Prob. 37PCh. 16 - Prob. 38PCh. 16 - Prob. 39PCh. 16 - Prob. 40PCh. 16 - Prob. 41PCh. 16 - The researchers who investigated bioluminescence...Ch. 16 - Prob. 43PCh. 16 - Quorum sensing controls the expression of...Ch. 16 - Scientists are currently screening a chemical...
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