Clary Leonhart used the pET vector system to express her prokaryotic amylase enzyme. added peptone into her culture broth of BL21(DE3) Escherichia coli strain. At the end of the experiment, she discovered that her protein was not expressed. She repeated three more times but her protein of interest was still not produced. (i) (ii) Explain the reason why Clary failed to obtain her protein of interest and suggest a solution to troubleshoot this problem. Clary plans to express her protein along with a polyhistidine-tag, or better known by its trademarked name IIis-tag. Explain the importance of His-tag in protein work.
Clary Leonhart used the pET vector system to express her prokaryotic amylase enzyme. added peptone into her culture broth of BL21(DE3) Escherichia coli strain. At the end of the experiment, she discovered that her protein was not expressed. She repeated three more times but her protein of interest was still not produced. (i) (ii) Explain the reason why Clary failed to obtain her protein of interest and suggest a solution to troubleshoot this problem. Clary plans to express her protein along with a polyhistidine-tag, or better known by its trademarked name IIis-tag. Explain the importance of His-tag in protein work.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:Clary Leonhart used the pET vector system to express her prokaryotic amylase enzyme. She
added peptone into her culture broth of BL21 (DE3) Escherichia coli strain. At the end of the
experiment, she discovered that her protein was not expressed. She repeated three more times
but her protein of interest was still not produced.
(i)
(ii)
Explain the reason why Clary failed to obtain her protein of interest and suggest a
solution to troubleshoot this problem.
Clary plans to express her protein along with a polyhistidine-tag, or better known by
its trademarked name IIis-tag. Explain the importance of His-tag in protein work.
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