The experimental design that makes the difference between the given two modes of action at a specific gene possible. Introduction: The antisense RNA is a type of single-stranded RNA molecule that binds to the complementary protein-coding (sense) mRNA molecule.

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Chapter 16, Problem 29P

Summary Introduction

To determine:

The experimental design that makes the difference between the given two modes of action at a specific gene possible.

Introduction:

The antisense RNA is a type of single-stranded RNA molecule that binds to the complementary protein-coding (sense) mRNA molecule.

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Here is the model for TfR mRNA regulation (see Fig.), for your convenience. Orange protein (IRP or IREBP), when bound to RNA, protects mRNA from degradation. When Fe is bound to the protein, it cannot bind the mRNA. If you made a radioactive DNA probe specific to TfR mRNA and performed a Northern blot analysis (to detect mRNA) from cells treated with high iron (Fe) concentration what would you expect to see? (a) Low iron RNase A (b) High iron O High levels of RNase O Low levels of TfR mRNA Fe RNase O The result would be the same as untreated cells Degradation O High levels of IRP (or IREBP - iron responsive element binding protein) O High levels of TfR mRNA

. One mechanism by which antisense RNAs act as negative regulators of gene expression is by base pairingwith the ribosome binding site on the sense mRNA toblock translation. In a second, alternative mechanism,the act of transcribing an antisense RNA can somehow prevent RNA polymerase from recognizing thesense promoter for the same gene. Design an experimental approach that would enable you to distinguishbetween these two modes of action at a specific gene.(Hint: What would be the outcome in each case ifhigh levels of the antisense RNA were transcribedfrom a gene on a plasmid?)

A newly identified protein from the cells of the Panopyra plant on Pandora was shown to inhibit translation of its target genes by binding to the 5’ UTR of the mRNA and preventing ribosome binding. A possible way this inhibition may be relieved by an sRNA would be: Group of answer choices 1.The sRNA acts as a marker, flagging the inhibitory protein for ubiquitination and allowing translation to take place. 2.The sRNA acts as a decoy, sequestering the inhibitory protein and allowing translation to take place. 3.The sRNA acts as an enhancer, suppressing the inhibitory protein and allowing translation to take place. 4.The sRNA acts as a silencer, suppressing the inhibitory protein and allowing translation to take place. Which one is the correct?

Answer 2

Question

Chapter 16, Problem 29P

Summary Introduction

To determine:

The experimental design that makes the difference between the given two modes of action at a specific gene possible.

Introduction:

The antisense RNA is a type of single-stranded RNA molecule that binds to the complementary protein-coding (sense) mRNA molecule.

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Answer 3

Question

Chapter 16, Problem 29P

Summary Introduction

To determine:

The experimental design that makes the difference between the given two modes of action at a specific gene possible.

Introduction:

The antisense RNA is a type of single-stranded RNA molecule that binds to the complementary protein-coding (sense) mRNA molecule.

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Answer 4

Question

Chapter 16, Problem 29P

Summary Introduction

To determine:

The experimental design that makes the difference between the given two modes of action at a specific gene possible.

Introduction:

The antisense RNA is a type of single-stranded RNA molecule that binds to the complementary protein-coding (sense) mRNA molecule.

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Answer 5

Chapter 16, Problem 29P

Summary Introduction

To determine:

The experimental design that makes the difference between the given two modes of action at a specific gene possible.

Introduction:

The antisense RNA is a type of single-stranded RNA molecule that binds to the complementary protein-coding (sense) mRNA molecule.

Answer 6

Chapter 16, Problem 29P

Summary Introduction

To determine:

The experimental design that makes the difference between the given two modes of action at a specific gene possible.

Introduction:

The antisense RNA is a type of single-stranded RNA molecule that binds to the complementary protein-coding (sense) mRNA molecule.

Answer 7

Chapter 16, Problem 29P

Summary Introduction

To determine:

The experimental design that makes the difference between the given two modes of action at a specific gene possible.

Introduction:

The antisense RNA is a type of single-stranded RNA molecule that binds to the complementary protein-coding (sense) mRNA molecule.

Answer 8

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Answer 11

Ch. 16 - For each of the terms in the left column, choose...Ch. 16 - The following statement occurs early in this...Ch. 16 - One of the main lessons of this chapter is that...Ch. 16 - All mutations that abolish function of the Rho...Ch. 16 - The figure at the beginning of this chapter shows...Ch. 16 - The promoter of an operon is the site to which RNA...Ch. 16 - You are studying an operon containing three genes...Ch. 16 - You have isolated a protein that binds to DNA in...Ch. 16 - You have isolated two different mutants reg1 and...Ch. 16 - Bacteriophage , after infecting a cell, can...

The experimental design that makes the difference between the given two modes of action at a specific gene possible. Introduction: The antisense RNA is a type of single-stranded RNA molecule that binds to the complementary protein-coding (sense) mRNA molecule.

Solution Summary: The author explains that the antisense RNA acts as negative regulators of gene expression by base-pairing with the ribosome binding site on the sense mRNA to block translation.

Concept explainers

Videos

To determine:

The experimental design that makes the difference between the given two modes of action at a specific gene possible.

Introduction:

The antisense RNA is a type of single-stranded RNA molecule that binds to the complementary protein-coding (sense) mRNA molecule.

Want to see the full answer?

Chapter 16 Solutions

The experimental design that makes the difference between the given two modes of action at a specific gene possible. Introduction: The antisense RNA is a type of single-stranded RNA molecule that binds to the complementary protein-coding (sense) mRNA molecule.

Solution Summary: The author explains that the antisense RNA acts as negative regulators of gene expression by base-pairing with the ribosome binding site on the sense mRNA to block translation.

Concept explainers

Videos

To determine: The experimental design that makes the difference between the given two modes of action at a specific gene possible. Introduction: The antisense RNA is a type of single-stranded RNA molecule that binds to the complementary protein-coding (sense) mRNA molecule.

Want to see the full answer?

Chapter 16 Solutions

To determine:

The experimental design that makes the difference between the given two modes of action at a specific gene possible.

Introduction:

The antisense RNA is a type of single-stranded RNA molecule that binds to the complementary protein-coding (sense) mRNA molecule.