The reason behind the promoter not exhibiting rotational symmetry. Introduction: The operator (o 1 ) and the binding site for CRP-cAMP present in the lac operon show rotational symmetry. On the contrary, the promoter does not exhibit rotational symmetry.

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Chapter 16, Problem 23P

Summary Introduction

To determine:

The reason behind the promoter not exhibiting rotational symmetry.

Introduction:

The operator (o₁) and the binding site for CRP-cAMP present in the lac operon show rotational symmetry. On the contrary, the promoter does not exhibit rotational symmetry.

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The diagram below represents the tryptophan operon with the trp leader mRNA transcript enlarged to represent the AUG translation start codon, two consecutive tryptophan amino acid codons (UGGUGG), and 4 regions (1, 2, 3, and 4) that base pair to form different hairpin-loop structures in the MRNA leader region. Suppose a mutant bacteria has region 4 of the trp operon attenuator region mutated so that it cannot base pair normally. Would the bacteria grow in the absence of the amino acid tryptophan? (hint: in order for bacteria to grow in absence of tryptophan it should be able to synthesize its own tryptophan) Lead&r region trpE trpD trpC trpB trpA DNA 5' 3' Transcription trp leader sequence MRNA UGGUGG 1 (tryptophan codons) AUG UUUUUU No There is insutficient information to answer the question. O Yes

To characterize the promoter of the gadA operon you made a series of deletion mutants removing pieces of the promoter to see what would happen. The results are found below: gad promoter gada gadX gadz 450 +1 lacz activity transcription start site pH 2.0 pH 7.0 A gad promoter beta-galactosidase (lacZ) +++ 450 gad promoter beta galactosidase (lacZ) +++ +++ 300 +1 gad promoter beta galactosidase (lacZ) 150 D gad beta galactosidase (lacz) -450 150 E gad promoter beta-galactosidase (lacZ) -450 -300 Based on these results, what can you conclude about the gad promoter? O a. The promoter is only regulated by repression Ob. The promoter is regulated by a mix of activation and repression O c. The promoter is only regulated by activation O d. The promoter has multiple operators and multiple enhancers

The diagram below represents the tryptophan operon with the trp leader mRNA transcript enlarged to represent the AUG translation start codon, two consecutive tryptophan amino acid codons (UGGUGG), and 4 regions (1, 2, 3, and 4) that base pair to form different hairpin-loop structures in the mRNA leader region. Suppose a mutant bacteria has region 3 of the trp operon attenuator region mutated so that it cannot base pair normally. Would the bacteria grow in the absence of the amino acid tryptophan? (hint: in order for bacteria to grow in absence of tryptophan it should be able to synthesize its own tryptophan) Leader region trpE trpD trpC trpB trpA DNA 5' 3' Transcription trp leader sequence MRNA AUG UGGUGG UUUUUU 1 2 3 (tryptophan codons) There is insufficient information to answer the question. Yes No O O

Answer 2

Question

Chapter 16, Problem 23P

Summary Introduction

To determine:

The reason behind the promoter not exhibiting rotational symmetry.

Introduction:

The operator (o₁) and the binding site for CRP-cAMP present in the lac operon show rotational symmetry. On the contrary, the promoter does not exhibit rotational symmetry.

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Answer 3

Question

Chapter 16, Problem 23P

Summary Introduction

To determine:

The reason behind the promoter not exhibiting rotational symmetry.

Introduction:

The operator (o₁) and the binding site for CRP-cAMP present in the lac operon show rotational symmetry. On the contrary, the promoter does not exhibit rotational symmetry.

Expert Solution & Answer

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Check out a sample textbook solution

See solution

Answer 4

Question

Chapter 16, Problem 23P

Summary Introduction

To determine:

The reason behind the promoter not exhibiting rotational symmetry.

Introduction:

The operator (o₁) and the binding site for CRP-cAMP present in the lac operon show rotational symmetry. On the contrary, the promoter does not exhibit rotational symmetry.

Expert Solution & Answer

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Check out a sample textbook solution

See solution

Answer 5

Chapter 16, Problem 23P

Summary Introduction

To determine:

The reason behind the promoter not exhibiting rotational symmetry.

Introduction:

The operator (o₁) and the binding site for CRP-cAMP present in the lac operon show rotational symmetry. On the contrary, the promoter does not exhibit rotational symmetry.

Answer 6

Chapter 16, Problem 23P

Summary Introduction

To determine:

The reason behind the promoter not exhibiting rotational symmetry.

Introduction:

The operator (o₁) and the binding site for CRP-cAMP present in the lac operon show rotational symmetry. On the contrary, the promoter does not exhibit rotational symmetry.

Answer 7

Chapter 16, Problem 23P

Summary Introduction

To determine:

The reason behind the promoter not exhibiting rotational symmetry.

Introduction:

The operator (o₁) and the binding site for CRP-cAMP present in the lac operon show rotational symmetry. On the contrary, the promoter does not exhibit rotational symmetry.

Answer 8

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Answer 11

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The reason behind the promoter not exhibiting rotational symmetry. Introduction: The operator (o 1 ) and the binding site for CRP-cAMP present in the lac operon show rotational symmetry. On the contrary, the promoter does not exhibit rotational symmetry.

Solution Summary: The author explains that the promoter does not exhibit rotational symmetry because the dimer binds to both the complementary strands of the lactose gene.

Concept explainers

Videos

To determine:

The reason behind the promoter not exhibiting rotational symmetry.

Introduction:

The operator (o₁) and the binding site for CRP-cAMP present in the lac operon show rotational symmetry. On the contrary, the promoter does not exhibit rotational symmetry.

Want to see the full answer?

Chapter 16 Solutions

The reason behind the promoter not exhibiting rotational symmetry. Introduction: The operator (o 1 ) and the binding site for CRP-cAMP present in the lac operon show rotational symmetry. On the contrary, the promoter does not exhibit rotational symmetry.

Solution Summary: The author explains that the promoter does not exhibit rotational symmetry because the dimer binds to both the complementary strands of the lactose gene.

Concept explainers

Videos

To determine: The reason behind the promoter not exhibiting rotational symmetry. Introduction: The operator (o1) and the binding site for CRP-cAMP present in the lac operon show rotational symmetry. On the contrary, the promoter does not exhibit rotational symmetry.

Want to see the full answer?

Chapter 16 Solutions

To determine:

The reason behind the promoter not exhibiting rotational symmetry.

Introduction:

The operator (o₁) and the binding site for CRP-cAMP present in the lac operon show rotational symmetry. On the contrary, the promoter does not exhibit rotational symmetry.