The process that can be performed to distinguish the genes involved in general stress response and the ones specific for osmolarity change. Introduction: One culture of E.coli is grown in a medium with high osmolarity, and another culture of E.coli is grown in a medium with low osmolarity to find the genes that are turned on or off in response to changes in osmolarity. RNA-seq analysis was performed for each culture. It was then hypothesized that osmotic changes might induce a general stress response that may be seen with other stressors like heat shock.

Question

Want to see the full answer?

Answer 1

Question

Chapter 16, Problem 37P

Summary Introduction

To determine:

The process that can be performed to distinguish the genes involved in general stress response and the ones specific for osmolarity change.

Introduction:

One culture of E.coli is grown in a medium with high osmolarity, and another culture of E.coli is grown in a medium with low osmolarity to find the genes that are turned on or off in response to changes in osmolarity. RNA-seq analysis was performed for each culture. It was then hypothesized that osmotic changes might induce a general stress response that may be seen with other stressors like heat shock.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

High salt concentrations tend to cause protein aggregation. Suggest a way to identify proteins normally expressed in particular bacterial species that can retain their solubility despite high salt conditions.

You plan to synthesize a peptide to be used as a vaccine to treat melanoma, a particularly aggressive form of skin cancer. Normally, gp100, a protein on the surface of melanocytes, activates cell growth when it is bound by its ligand. Activation of the growth pathway depends on the presence of threonine in the ligand. The effective peptide vaccine will mimic the natural ligand, but won’t cause cell growth and division. Below is the sequence of the natural ligand: LDMKTAG In order to ensure your newly designed peptide vaccine does not cause cell growth upon binding, you must substitute the Threonine residue at position 5. What amino acid would you replace it with, bearing in mind that the peptide should still be similar enough to bind to the gp100 protein in the surface of melanocytes. Explain your choice. Your vaccine will be administered as a topical cream, and you require your peptide to have an overall neutral charge in order to be functional. At what pH should you formulate…

The codon change (Gly-12 to Val-12) in human rasH that convertsit to oncogenic rasH has been associated with many types ofcancers. For this reason, researchers would like to develop drugs toinhibit oncogenic rasH. Based on your understanding of the Rasprotein, what types of drugs might you develop? In other words,what would be the structure of the drugs, and how would theyinhibit Ras protein? How would you test the efficacy of the drugs?What might be some side effects?