Genetics: From Genes to Genomes
6th Edition
ISBN: 9781259700903
Author: Leland Hartwell Dr., Michael L. Goldberg Professor Dr., Janice Fischer, Leroy Hood Dr.
Publisher: McGraw-Hill Education
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Chapter 16, Problem 12P
Suppose you have six strains of E. coli. One is wild type, and each of the other five has a single one of the following mutations: lacZ-, lacY-, lacI->, oc>, and lacIs> . For each of these six strains, describe the
| a. Growth on medium in which the only carbon source was lactose. |
| b. Colony color in medium containing glycerol as the only carbon source, X-gal, and IPTG. |
| c. Colony color in medium containing glycerol as the only carbon source and X-gal, but no IPTG. |
| d. Colony color in medium containing high levels of glucose as the only carbon source, X-gal, and IPTG. |
| e. Colony color in medium containing high levels of glucose as the only carbon source and X-gal, but no IPTG. |
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A classic way to isolate thymidylate synthase-negative mutants of bacteria
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Medium
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Clary Fray used the pET vector system to express her prokaryotic amylase enzyme. She added
peptone into her culture broth of BL21(DE3) Escherichia coli strain to induce protein
expression. At the end of the experiment, she discovered that her protein was not expressed.
She repeated three more times but her protein of interest was still not produced.
(i)
(ii)
(iii)
(iv)
(v)
Explain the reason why Clary failed to obtain her protein of interest and suggest a
solution to troubleshoot this problem.
Clary plans to express her protein along with a polyhistidine-tag. Explain the
importance of His-tag in protein work.
Is DH5a Escherichia coli suitable to propagate the plasmid before protein expression?
Besides heat shock method, elaborate another procedure Clary could utilize to
transform the recombinant pET vector into the host cell.
If her supervisor instructs her to express a gene from gold fish (Carassius auratus), is
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Chapter 16 Solutions
Genetics: From Genes to Genomes
Ch. 16 - For each of the terms in the left column, choose...Ch. 16 - The following statement occurs early in this...Ch. 16 - One of the main lessons of this chapter is that...Ch. 16 - All mutations that abolish function of the Rho...Ch. 16 - The figure at the beginning of this chapter shows...Ch. 16 - The promoter of an operon is the site to which RNA...Ch. 16 - You are studying an operon containing three genes...Ch. 16 - You have isolated a protein that binds to DNA in...Ch. 16 - You have isolated two different mutants reg1 and...Ch. 16 - Bacteriophage , after infecting a cell, can...
Ch. 16 - Mutants were isolated in which the constitutive...Ch. 16 - Suppose you have six strains of E. coli. One is...Ch. 16 - The previous problem raises some interesting...Ch. 16 - For each of the E. coli strains containing the lac...Ch. 16 - For each of the following growth conditions, what...Ch. 16 - For each of the following mutant E. coli strains,...Ch. 16 - Maltose utilization in E. coli requires the...Ch. 16 - Seven E. coli mutants were isolated. The activity...Ch. 16 - Cells containing missense mutations in the crp...Ch. 16 - Six strains of E.coli mutants 16 that had one of...Ch. 16 - a. The original constitutive operator mutations in...Ch. 16 - In an effort to determine the location of an...Ch. 16 - Prob. 23PCh. 16 - The footprinting experiment described in Fig....Ch. 16 - Why is the trp attenuation mechanism unique to...Ch. 16 - a. How many ribosomes are required at a minimum...Ch. 16 - The following is a sequence of the leader region...Ch. 16 - For each of the E. coli strains that follow,...Ch. 16 - Prob. 29PCh. 16 - For each element in the list that follows,...Ch. 16 - Among the structurally simplest riboswitches are...Ch. 16 - Great variation exists in the mechanisms by which...Ch. 16 - Many genes whose expression is turned on by DNA...Ch. 16 - In 2005, Frederick Blattner and his colleagues...Ch. 16 - The E.coli MalT protein is a positive regulator of...Ch. 16 - Prob. 36PCh. 16 - Prob. 37PCh. 16 - Prob. 38PCh. 16 - Prob. 39PCh. 16 - Prob. 40PCh. 16 - Prob. 41PCh. 16 - The researchers who investigated bioluminescence...Ch. 16 - Prob. 43PCh. 16 - Quorum sensing controls the expression of...Ch. 16 - Scientists are currently screening a chemical...
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