Genetics: From Genes to Genomes
6th Edition
ISBN: 9781259700903
Author: Leland Hartwell Dr., Michael L. Goldberg Professor Dr., Janice Fischer, Leroy Hood Dr.
Publisher: McGraw-Hill Education
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Chapter 14, Problem 23P
Starting with an F- strain that was prototrophic (that is, had no auxotrophic mutations) and Strs, several independent Hfr strains were isolated. These Hfr strains were mated to an F- strain that was Strr Arg- Cys- His- Ilv- Lys- Met- Nic- Pab- Pyr- Trp-. Interrupted-mating experiments showed that the Hfr strains transferred the wild-type alleles in the order listed in the following table as a function of time. The time of entry for the markers within parentheses could not be distinguished from one another.
| a. | From these data, derive a map of the relative position of these markers on the bacterial chromosome. Indicate with labeled arrows the position and orientation of the integrated F plasmid for each Hfr strain. |
| b. | To determine the relative order of the trp, pyr, and cys markers and the distances between them, HfrB was mated with the F- strain long enough to allow transfer of the nic marker, after which Trp+ recombinants were selected. The unselected markers pyr and cys were then scored in the Trp+ recombinants were selected. The unselected markers pyr and cys were then scored in the Trp+ recombinants, yielding the following results: |
Draw a map of the trp, pyr, and cys markers relative to each other. (Note that you cannot determine the order relative to the nic or his genes using these data.) Express map distances between adjacent genes as the frequency of crossing-over between them.
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Students have asked these similar questions
Wild-type bacteria were used as donors to transduce the
following recipient genotypes with the results indicated as
the number of wild type colonies:
Recipient
Recipient
leu- phe+ his- met+ tyr+
leu- phe+ his+ met- tyr+
leu- phe+ his+ met+ tyr-
leu- phe- his+ met+ tyr+
leu+ phe+ his-met- tyr+
127
leu+ phe-his- met+ tyr+
#wild type
37
138
132
1
leu+ phe+ his- met+ tyr-
leu+ phe-his+ met- tyr+
leu+ phe-hist met+ tyr-
leu- phe+ his+ met+ tyr+
22
6
leu+ phe+ his+ met- tyr-
148
31
304
leu+ phe+ his+ met+ tyr-
What is the order of the genes?
tyr-leu-his-phe-met
O leu-met-phe-his-tyr
leu-his-met-tyr-phe
his-tyr-leu-phe-met
#wild type
356
10
Eight mutant bacteriophage strains cannot lyse a certain type of bacteria that can be lysed by
wild-type bacteriaphages. The mutant strains were allawed to infect the bacteria in a
complementation test. A "+ indicates that lysis occurred with coinfection.Aindicates that
lysis did not occur.
GKWTMAQC
G -++|+ ++l+ -
K.
- + - ++ +| +
-+| - |-++
+ + +
M
- +
+
+
A cistron is defined by no complementation in the
Ciş
: configuration.
Haw many genes are controlling lysis in this bacteriophage? (Use a number not a word in the
space) 3
Which of the following strains below are defective in the same gene as strain Q? Answer yes
if the strain is defective or no if it is not.
Strain Defective
A
N/A
In Escherichia coli, four different Hfr strains, derived from the same F* strains, were mated with F
strains auxotrophic for a number of nutritional requirements (Arg Bio Cys Trp Gal His Lac Mal
Xyl Leu Met). Matings were interrupted at various intervals and cells were plated on minimal
medium supplemented with particular nutrients to test for gene transfer. The following results show
the time of entry for each of the genes in four different Hfr strains.
Table 1: Time-of-Entry Mapping Data*
Hfr strains
Genes
lac"
his"
arg' bio
9.
Cys
gal"
trp'
5
11.5 2.5
mal" xyl"
leu
met
Hfr 1
Hfr 2
Hfr 3
Hfr 4
6.5
3.5
11
15
15
4
6.
17.5
5
14.5
3
20
* The numbers denote the number of minutes elapsed before a gene enters the F
cells.
Draw a circular map of E. coli chromosome.
Chapter 14 Solutions
Genetics: From Genes to Genomes
Ch. 14 - Choose the phrase from the right column that best...Ch. 14 - The unicellular, rod-shaped bacterium E. coli is 2...Ch. 14 - Now that the sequence of the entire E. coli K12...Ch. 14 - Bacterial genomes such as that of E. coli...Ch. 14 - List at least three features of eukaryotic genomes...Ch. 14 - Describe a mechanism by which a gene could move...Ch. 14 - High salt concentrations tend to cause protein...Ch. 14 - Recently, scientists tested the possibility that...Ch. 14 - A recent metagenomic study analyzed the...Ch. 14 - Linezolid is a new type of antibiotic that...
Ch. 14 - A liquid culture of E. coli at a concentration of...Ch. 14 - Pick out the medium i, ii, iii, or iv onto which...Ch. 14 - This problem concerns Fig. 14.14, which...Ch. 14 - In two isolates one is resistant to ampicillin,...Ch. 14 - E. coli cells usually have only one copy of the F...Ch. 14 - In E. coli, the genes purC and pyrB are located...Ch. 14 - DNA sequencing of the entire H. influenzae genome...Ch. 14 - Genes encoding toxins are often located on...Ch. 14 - a. You want to perform an interrupted-mating...Ch. 14 - In Problem 19, do you think that most of the...Ch. 14 - One issue with interrupted-mating experiments such...Ch. 14 - Prob. 22PCh. 14 - Starting with an F- strain that was prototrophic...Ch. 14 - You can carry out matings between an Hfr and F...Ch. 14 - Genome sequences show that some pathogenic...Ch. 14 - Generalized and specialized transduction both...Ch. 14 - This problem highlights some useful variations of...Ch. 14 - A researcher has a Trp auxotrophic strain of E....Ch. 14 - Streptococcus parasanguis is a bacterial species...Ch. 14 - The sequence at one end of one strand of the...Ch. 14 - Scientists who study amino acid biosynthesis...Ch. 14 - Suppose that you could obtain radioactively...Ch. 14 - Prob. 34PCh. 14 - Some scientists are trying to engineer...
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