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Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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
Transcribed Image Text:A donor strain of bacteria with genotype leu* gal pro* is infected
with phages. The phage lysate from the bacterial cells is collected
and used to infect a second strain of bacteria that are leu gal* pro".
The second strain is selected for leu", and the following
cotransduction data are obtained:
Cells with
cotransduced
allele (%)
Selected
Donor Recipient
allele
leu*
leu" gal*
leu*
47 pro*
gal"
pro*
pro
leu*
26 gal"
Which genes are closest, leu and gal or leu and pro?
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- You co-culture the following bacterial strains: an Hfr prototroph and an F- auxotroph for the genes mal, met, mtl, and xyl. You interrupt conjugation at various time points and place the mixtures on media plates lacking each of the nutrients. Based on the results shown in the image, what is the order of these four genes along the bacterial chromosome? Please explain!arrow_forwardIf a student uses a monoclonal antibody to detect a protein using a direct Western-blotting protocol after running an SDS PAGE, the student can detect a 50kd protein. However, if the student tries to use this antibody to immunoprecipitate the 50 kd protein and then Western -blot the immunoprecipitated samples, the student found that there is no band at all at 50kd region in the Western blot. How do you explain these results based on what you know about protein structure and antigen-antibody recognition? (Hint: Western blot analysis explores an SDS-containing buffer, whereas the immunoprecipitation buffer uses a NP40-containing buffer to maintain proteins in their native form).arrow_forwardWe isolate DNA from a bacteria that is wild type for the genes arg leu and his shear it in a blender mix it into a culture of bacteria that is mutant for the genes arg leu and his . We then plate on various media. These are the results: Media with arginine 20 colonies /ml Media with histidine 300 colonies /ml Media with leucine 500 colonies /ml Media with all three 10000 colonies/ml 21. What is this experiment called? A. transpiration B. conjugation C. transformation D. transductionarrow_forward
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