200 mal+ 150 100 mtl+ 50 met+ 0. 40 60 Time of sampling (minutes) 20 80 100 Number of recombinants per milliliter (×104)

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Austin Taylor and Edward Adelberg isolated some new strains of Hfr cells that they then used to map several genes in E. coli by using interrupted conjugation . In one experiment, they mixed cells of Hfr strain AB-312, which were xyl+ mtl+ mal+ met+ and sensitive to phage T6, with F strain AB-531, which was xyl mtl mal met and resistant to phage T6. The cells were allowed to undergo conjugation. At regular intervals, the researchers removed a sample of cells and interrupted conjugation by killing the Hfr cells with phage T6. The F cells, which were resistant to phage T6, survived
and were then tested for the presence of genes transferred from the Hfr strain. The results of this experiment are shown in the accompanying graph. On the basis of these data, give the order of the xyl, mtl, mal, and met genes on the bacterial chromosome and indicate the minimum distances between them.

200
mal+
150
100
mtl+
50
met+
0.
40
60
Time of sampling (minutes)
20
80
100
Number of recombinants
per milliliter (×104)
Transcribed Image Text:200 mal+ 150 100 mtl+ 50 met+ 0. 40 60 Time of sampling (minutes) 20 80 100 Number of recombinants per milliliter (×104)
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