a. To draw: The map that shows relative positions of markers and origins of transfer in HfrA and HfrB strains and also the distance between these markers. Introduction: Genetic markers are the genes that are identifiable through phenotypic variants. These markers serve as a point of reference in deciding whether the progeny is the result of recombination.

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Chapter 14, Problem 22P

Summary Introduction

a.

To draw:

The map that shows relative positions of markers and origins of transfer in HfrA and HfrB strains and also the distance between these markers.

Introduction:

Genetic markers are the genes that are identifiable through phenotypic variants. These markers serve as a point of reference in deciding whether the progeny is the result of recombination.

Summary Introduction

b.

To draw:

The order of the genes based on the given cotransduction data.

Introduction:

Cotransduction refers to the transfer of different bacterial genes together in one phage by transduction process.

Summary Introduction

c.

To determine:

The way an individual increases the chances of mapping gly gene relative to at least some of the other markers and the composition of the medium that would be used to map gly.

Introduction:

Tranductants refer to the cells resulting from the gene transfer. Transduction process is mainly mediated by bacteriophages.

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Schizosaccharomyces pombe, also known as "fission yeast," is a powerful model organism in molecular and cell biology. While performing a genetic screen, you discover an auxotrophic S. pombe strain that is unable to synthesize one or more vitamins. The following table represents the key experiments you performed during your genetic screen. Fill in the table with the outcome of each experiment for your mutant strain (using + for growth and - for no growth). Medium Rich media Minimal media Minimal media + all vitamins Minimal media + all amino acids Growth Wild-type + + + + Mutant + + + > > >

A prototrophic strain (his" arg" lac') was used as a donor to transform an auxotrophic strain (his arg lac'). Initial transformants are isolated on minimal medium + histidine + arginine + lactose - glucose. i. What genotypes will grow on this medium? ii. These colonies are replicated to minimal medium + histidine, and 50% of the original colonies grow. What genotypes will grow on this medium? iii. The original colonies are also replicated to minimal medium + arginine, and 10% of the colonies grow. What genotypes will grow on this medium? iv. The original colonies are also replicated to minimal medium. No colonies grow. Based on this information, what genotypes will grow on minimal medium + histidine and on minimal medium + arginine? What is the relative gene order for his, arg, and lac? Which two genes are closer? Explain your answer.

Given what we've discussed in class, what will be most likely outcome if you conjugate an streptomycin resistant ampicillin sensitive methionine auxotroph E. coli strain (engineered to be pir+) that is F- with a streptomycin sensitive non-HFR methionine prototroph strain that is F- and RP4+ but contains pUC18? Colonies on minimal media + ampicillin +streptomycin plates No colonies on minimal media +ampicillin +streptomycin plates

Answer 2

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Chapter 14, Problem 22P

Summary Introduction

a.

To draw:

The map that shows relative positions of markers and origins of transfer in HfrA and HfrB strains and also the distance between these markers.

Introduction:

Genetic markers are the genes that are identifiable through phenotypic variants. These markers serve as a point of reference in deciding whether the progeny is the result of recombination.

Summary Introduction

b.

To draw:

The order of the genes based on the given cotransduction data.

Introduction:

Cotransduction refers to the transfer of different bacterial genes together in one phage by transduction process.

Summary Introduction

c.

To determine:

The way an individual increases the chances of mapping gly gene relative to at least some of the other markers and the composition of the medium that would be used to map gly.

Introduction:

Tranductants refer to the cells resulting from the gene transfer. Transduction process is mainly mediated by bacteriophages.

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Answer 3

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Chapter 14, Problem 22P

Summary Introduction

a.

To draw:

The map that shows relative positions of markers and origins of transfer in HfrA and HfrB strains and also the distance between these markers.

Introduction:

Genetic markers are the genes that are identifiable through phenotypic variants. These markers serve as a point of reference in deciding whether the progeny is the result of recombination.

Summary Introduction

b.

To draw:

The order of the genes based on the given cotransduction data.

Introduction:

Cotransduction refers to the transfer of different bacterial genes together in one phage by transduction process.

Summary Introduction

c.

To determine:

The way an individual increases the chances of mapping gly gene relative to at least some of the other markers and the composition of the medium that would be used to map gly.

Introduction:

Tranductants refer to the cells resulting from the gene transfer. Transduction process is mainly mediated by bacteriophages.

Expert Solution & Answer

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Answer 4

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Chapter 14, Problem 22P

Summary Introduction

a.

To draw:

The map that shows relative positions of markers and origins of transfer in HfrA and HfrB strains and also the distance between these markers.

Introduction:

Genetic markers are the genes that are identifiable through phenotypic variants. These markers serve as a point of reference in deciding whether the progeny is the result of recombination.

Summary Introduction

b.

To draw:

The order of the genes based on the given cotransduction data.

Introduction:

Cotransduction refers to the transfer of different bacterial genes together in one phage by transduction process.

Summary Introduction

c.

To determine:

The way an individual increases the chances of mapping gly gene relative to at least some of the other markers and the composition of the medium that would be used to map gly.

Introduction:

Tranductants refer to the cells resulting from the gene transfer. Transduction process is mainly mediated by bacteriophages.

Expert Solution & Answer

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Answer 6

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Answer 7

Chapter 14, Problem 22P

Summary Introduction

a.

To draw:

The map that shows relative positions of markers and origins of transfer in HfrA and HfrB strains and also the distance between these markers.

Introduction:

Genetic markers are the genes that are identifiable through phenotypic variants. These markers serve as a point of reference in deciding whether the progeny is the result of recombination.

Summary Introduction

b.

To draw:

The order of the genes based on the given cotransduction data.

Introduction:

Cotransduction refers to the transfer of different bacterial genes together in one phage by transduction process.

Summary Introduction

c.

To determine:

The way an individual increases the chances of mapping gly gene relative to at least some of the other markers and the composition of the medium that would be used to map gly.

Introduction:

Tranductants refer to the cells resulting from the gene transfer. Transduction process is mainly mediated by bacteriophages.

Answer 8

Chapter 14, Problem 22P

Summary Introduction

a.

To draw:

The map that shows relative positions of markers and origins of transfer in HfrA and HfrB strains and also the distance between these markers.

Introduction:

Genetic markers are the genes that are identifiable through phenotypic variants. These markers serve as a point of reference in deciding whether the progeny is the result of recombination.

Answer 9

Chapter 14, Problem 22P

Summary Introduction

a.

To draw:

The map that shows relative positions of markers and origins of transfer in HfrA and HfrB strains and also the distance between these markers.

Introduction:

Genetic markers are the genes that are identifiable through phenotypic variants. These markers serve as a point of reference in deciding whether the progeny is the result of recombination.

Answer 10

Summary Introduction

b.

To draw:

The order of the genes based on the given cotransduction data.

Introduction:

Cotransduction refers to the transfer of different bacterial genes together in one phage by transduction process.

Answer 11

Summary Introduction

b.

To draw:

The order of the genes based on the given cotransduction data.

Introduction:

Cotransduction refers to the transfer of different bacterial genes together in one phage by transduction process.

Answer 12

Summary Introduction

c.

To determine:

The way an individual increases the chances of mapping gly gene relative to at least some of the other markers and the composition of the medium that would be used to map gly.

Introduction:

Tranductants refer to the cells resulting from the gene transfer. Transduction process is mainly mediated by bacteriophages.

Answer 13

Summary Introduction

c.

To determine:

The way an individual increases the chances of mapping gly gene relative to at least some of the other markers and the composition of the medium that would be used to map gly.

Introduction:

Tranductants refer to the cells resulting from the gene transfer. Transduction process is mainly mediated by bacteriophages.

Answer 14

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Answer 15

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Answer 16

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Answer 17

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Solution Summary: The author explains how to draw the order of the genes based on the given cotransduction data.

b.

To draw:

The order of the genes based on the given cotransduction data.

Introduction:

Cotransduction refers to the transfer of different bacterial genes together in one phage by transduction process.

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Chapter 14 Solutions

Solution Summary: The author explains how to draw the order of the genes based on the given cotransduction data.

b. To draw: The order of the genes based on the given cotransduction data. Introduction: Cotransduction refers to the transfer of different bacterial genes together in one phage by transduction process.

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Chapter 14 Solutions

b.

To draw:

The order of the genes based on the given cotransduction data.

Introduction:

Cotransduction refers to the transfer of different bacterial genes together in one phage by transduction process.