Genetics: From Genes to Genomes
6th Edition
ISBN: 9781259700903
Author: Leland Hartwell Dr., Michael L. Goldberg Professor Dr., Janice Fischer, Leroy Hood Dr.
Publisher: McGraw-Hill Education
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Chapter 14, Problem 17P
DNA sequencing of the entire H. influenzae genome was completed in 1995. When DNA from the nonpathogenic strain H. influenzae Rd was compared to that of the pathogenic b strain, eight genes of the fimbrial gene cluster (located between the purE and pepN genes) involved in adhesion of bacteria to host cells were completely missing from the nonpathogenic strain. What effect would this deletion have on cotransformation of purE and pepN genes using DNA isolated from the nonpathogenic versus the pathogenic strain?
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DNA sequencing of the entire H. influenzae genomewas completed in 1995. When DNA from the nonpathogenic strain H. influenzae Rd was compared tothat of the pathogenic b strain, eight genes of the fimbrial gene cluster (located between the purE andpepN genes) involved in adhesion of bacteria to hostcells were completely missing from the nonpathogenic strain. What effect would this deletion have oncotransformation of purE and pepN genes using DNAisolated from the nonpathogenic versus the pathogenic strain?
Bacteriophage P22 was used in generalized transduction experiments to infect the Salmonella
typhimurium donor strains described in the table below. The resulting phage lysates were then
used to infect the S. typhimurium recipient strains listed in the table. In each cross, a
phenotype was selected for one of the three genetic markers studied (str, aceA, thrA), and then
replicates were performed to select the corresponding recombinants for the other two markers.
The results are given in the following table:
Recipient strain Selected phenotype Selected recombinants
Donor
strain
str thrA
aceA+ thrA
str aceA+
strs thrA+
aceA thrA+
str aceA
Str
Ace+
Str
ThrA
ThrA+
ThrA
ThrA+
Ace
Ace
str: gene involved in streptomycin resistance,
aceA gene involved in the use of acetate as a carbon source,
thrA: gene involved in the biosynthesis of threonine.
Number
60
40
95
5
10
90
Determine the order of the genes and draw a genetic map showing this order
Bacteriophage P22 was used in generalised transduction experiments to infect the Salmonella
typhimurium donor strains described in the table below.
The resulting phage lysates were then used to infect the recipient strains of S. typhimurium
recipient strains listed in the table.
In each cross, a phenotype was selected for one of the selected for one of the three genetic
markers studied (str, aceA, thrA), and were made to select the recombinants corresponding to the
other two markers. markers.
The results are given in the following table:
Strain
I donor
str thrA
aceA thrA
str aceA+
Strain
recipient
strs thrA+
aceA thrA
str aceA
Phenotype
selected
Str
Ace+
Str
recombinants
selected
ThrA
ThrA
ThrA
ThrA
Ace
Ace
Number
60
40
95
5
10
90
str: gene involved in streptomycin resistance,
aceA: gene involved in the use of acetate as a carbon source,
thrA: gene involved in threonine biosynthesis.
1) What are the selective media used in these three transduction experiments? to obtain the
selected…
Chapter 14 Solutions
Genetics: From Genes to Genomes
Ch. 14 - Choose the phrase from the right column that best...Ch. 14 - The unicellular, rod-shaped bacterium E. coli is 2...Ch. 14 - Now that the sequence of the entire E. coli K12...Ch. 14 - Bacterial genomes such as that of E. coli...Ch. 14 - List at least three features of eukaryotic genomes...Ch. 14 - Describe a mechanism by which a gene could move...Ch. 14 - High salt concentrations tend to cause protein...Ch. 14 - Recently, scientists tested the possibility that...Ch. 14 - A recent metagenomic study analyzed the...Ch. 14 - Linezolid is a new type of antibiotic that...
Ch. 14 - A liquid culture of E. coli at a concentration of...Ch. 14 - Pick out the medium i, ii, iii, or iv onto which...Ch. 14 - This problem concerns Fig. 14.14, which...Ch. 14 - In two isolates one is resistant to ampicillin,...Ch. 14 - E. coli cells usually have only one copy of the F...Ch. 14 - In E. coli, the genes purC and pyrB are located...Ch. 14 - DNA sequencing of the entire H. influenzae genome...Ch. 14 - Genes encoding toxins are often located on...Ch. 14 - a. You want to perform an interrupted-mating...Ch. 14 - In Problem 19, do you think that most of the...Ch. 14 - One issue with interrupted-mating experiments such...Ch. 14 - Prob. 22PCh. 14 - Starting with an F- strain that was prototrophic...Ch. 14 - You can carry out matings between an Hfr and F...Ch. 14 - Genome sequences show that some pathogenic...Ch. 14 - Generalized and specialized transduction both...Ch. 14 - This problem highlights some useful variations of...Ch. 14 - A researcher has a Trp auxotrophic strain of E....Ch. 14 - Streptococcus parasanguis is a bacterial species...Ch. 14 - The sequence at one end of one strand of the...Ch. 14 - Scientists who study amino acid biosynthesis...Ch. 14 - Suppose that you could obtain radioactively...Ch. 14 - Prob. 34PCh. 14 - Some scientists are trying to engineer...
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- a. Bacteriophage P22 was used in generalized transduction experiments to infect the Salmonella typhimurium donor strains described in the table below. The resulting phage lysates were then used to infect the S. typhimurium recipient strains listed in the table. In each cross, a phenotype was selected for one of the three genetic markers studied (str, aceA, thrA), and then replicates were performed to select the corresponding recombinants for the other two markers. The results are given in the following table: Recipient strain Selected phenotype Selected recombinants Donor strain str thrA aceAt thrA str aceA+ strs thrA+ aceA thrA+ str aceA Str Ace+ Str ThrA ThrA+ ThrA ThrA+ Ace Ace+ str: gene involved in streptomycin resistance, aceA: gene involved in the use of acetate as a carbon source, thrA: gene involved in the biosynthesis of threonine. Number 60 40 95 5 10 90 What are the selective media used in these three transduction experiments, on the one hand to obtain the selected…arrow_forwardHelp mearrow_forwardBy conducting conjugation experiments between Hfr and recipientstrains, Wollman and Jacob mapped the order of many bacterialgenes. Throughout the course of their studies, they identified severaldifferent Hfr strains in which the F-factor DNA had been integratedat different places along the bacterial chromosome. A sample of theirexperimental results is shown in the following table:Explain how these results are consistent with the idea that thebacterial chromosome is circular?arrow_forward
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- Austin Taylor and Edward Adelberg isolated some new strains of Hfr cells that they then used to map several genes in Escherichia coli by using interrupted conjugation. In one experiment, the researchers mixed cells of Hfr strain AB‑312, which were xyl+ mtl+ mal+ met+ and sensitive to phage T6, with F− strain AB‑531, which was xyl− mtl− mal− met− and resistant to phage T6. The cells were allowed to undergo conjugation. At regular intervals, the researchers removed a sample of cells and interrupted conjugation by killing the Hfr cells with phage T6. The F− cells, which were resistant to phage T6, survived and were then tested for the presence of genes transferred from the Hfr strain. The results of this experiment are shown in the graph. On the basis of these data, give the order of the xyl, mtl, mal, and met genes on the bacterial chromosome and the minimum distances between them in minutes. The origin of transfer is represented by the red triangle. The distances between genes are not…arrow_forwardTwo mutations that affect plaque morphology in phages (a and b ) have been isolated. Phages carrying both mutations (a b) are mixed with wild-type phages (a* b*) and added to a culture of bacterial cells. Once the phages have infected and lysed the bacteria, samples of the phage lysate are collected and cultured on plated bacteria. The following numbers of plaques are observed: Plaque phenotype Number a* b* 2043 a* b- 320 a b* 357 2134 What is the frequency of recombination between the a and b genes?arrow_forwardF ′strains in E. coli are derived from Hfr strains. In some cases, these F ′strains show a high rate of integration back into the bacterial chromosome of a second strain. Furthermore, the site of integration is often the site occupied by the sex factor in the original Hfr strain (before production of the F ′strains). Explain these results.arrow_forward
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