Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 10, Problem 7EQ
If you were given a sample of chromosomal DNA and asked to determine if it is bacterial or eukaryotic, what experiment would you perform, and what would be your expected results?
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Students have asked these similar questions
You are trying to determine the base content for a number of samples in the lab (mouse DNA, bacterial DNA, single-stranded DNA virus and double-stranded RNA virus). Unfortunately, you failed to use an alcohol-proof marker and during the ethanol precipitation of your samples the labels came off the tubes. You finish the experiment anyway and try to use the data to determine the samples. Can you determine the source of each sample based on your results? Briefly explain how you come to your designations.
Sample
% adenine
% cytosine
% guanine
% thymine
% uracil
A
18
32
32
18
0
B
26
18
32
24
0
C
31
19
19
0
31
D
10
40
40
10
0
Neema wants to determine whether or not the nucleus of a cell differs in the chemical compounds they contain or not from species to species. She is planning on working with the nucleus of a human and the nucleus of a mouse. She has removed all of the DNA from each nucleus, then has selected and isolated one DNA fragment from each species. What results can Neema expect if she takes the human DNA fragment and inserts it into the mouse nucleus and then inserts the mouse DNA fragment into the human nucleus? Use your knowledge of DNA replication to answer.
There are three basic steps of DNA extraction: cell lysis, separation, and extraction. In the procedure for extracting DNA from human cheek cells, which parts belong to or explain the aforementioned three basic steps?
You may refer to the following procedure:
http://www.planet-science.com/categories/experiments/biology/2012/03/extract-your-own-dna.aspx
Chapter 10 Solutions
Genetics: Analysis and Principles
Ch. 10.1 - 1. A bacterial chromosome typically contains
a. a...Ch. 10.2 - Mechanisms that make the bacterial chromosome more...Ch. 10.2 - 2. Negative supercoiling may enhance activities...Ch. 10.2 - 3. DNA gyrase
a. promotes negative supercoiling....Ch. 10.3 - 1. The chromosomes of eukaryotes typically contain...Ch. 10.4 - Which of the following is an example of a...Ch. 10.5 - What are the components of a single nucleosome? a....Ch. 10.5 - 2. In Noll’s experiment to test the...Ch. 10.5 - Prob. 3COMQCh. 10.5 - Prob. 4COMQ
Ch. 10.6 - Prob. 1COMQCh. 10.6 - 2. The role of cohesin is to
a. make chromosomes...Ch. 10 - Prob. 1CONQCh. 10 - Prob. 2CONQCh. 10 - 3. Describe the mechanisms by which bacterial DNA...Ch. 10 - Why is DNA supercoiling called supercoiling rather...Ch. 10 - Prob. 5CONQCh. 10 - Prob. 6CONQCh. 10 - Prob. 7CONQCh. 10 - Prob. 8CONQCh. 10 - Prob. 9CONQCh. 10 - 10. What is the function of a centromere? At what...Ch. 10 - Prob. 11CONQCh. 10 - 12. Describe the structures of a nucleosome and a...Ch. 10 - Beginning with the G1 phase of the cell cycle,...Ch. 10 - Draw a picture depicting the binding between the...Ch. 10 - 15. Compare heterochromatin and euchromatin. What...Ch. 10 - 16. Compare the structure and cell localization of...Ch. 10 - 17. What types of genetic activities occur during...Ch. 10 - Lets assume the linker region of DNA averages 54bp...Ch. 10 - 19. In Figure 10.12, what are we looking at in...Ch. 10 - 20. What are the roles of the core histone...Ch. 10 - A typical eukaryotic chromosome found in humans...Ch. 10 - Which of the following terms should not be used to...Ch. 10 - Discuss the differences between the compaction...Ch. 10 - 24. What is an SMC complex? Describe two...Ch. 10 - Two circular DNA molecules, which we can call...Ch. 10 - 2. Let’s suppose you have isolated DNA from a cell...Ch. 10 - 3. We seem to know more about the structure of...Ch. 10 - In Nolls experiment of Figure 10.11, explain where...Ch. 10 - When chromatin is treated with a salt solution of...Ch. 10 - 6. Let’s suppose you have isolated chromatin from...Ch. 10 - If you were given a sample of chromosomal DNA and...Ch. 10 - Consider how histone proteins bind to DNA and then...Ch. 10 - In Chapter 23, the technique of fluorescence in...Ch. 10 - Bacterial and eukaryotic chromosomes are very...Ch. 10 - The prevalence of highly repetitive sequences...Ch. 10 - Discuss and make a list of the similarities and...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Astronomers have made great strides in sending probes out to other planets and moons in our solar system. If they were to find a living creature some place other than Earth, how could DNA analysis help them better understand the organism? Explain in 1-2 sentences. B I U !!!arrow_forwardYou are provided with a sample of aardvark DNA. As part of your investigation of this DNA, you transcribe mRNA from the DNA and purify it. You then separate the two strands of the DNA and analyze the base composition of each strand and of the mRNA transcripts. You obtain the following results: A G C T UDNA strand #1 19.1 26.0 31.0 23.9 0DNA strand #1 24.2 30.8 25.7 19.3 0mRNA 19.0 25.9 30.8 0 24.3Which strand of the DNA is the “sense” strand that serves as the template for mRNA synthesis?arrow_forwardWhy do we study the DNA sequence of human organism?arrow_forward
- Astronomers have made great strides in sending probes out to other planets and moons in our solar system. If they were to find a living creature some place other than Earth, how could DNA analysis help them better understand the organism? Explain in 1–2 sentencesarrow_forwardwhat do you think they are trying to determine in this experiment?arrow_forwardEach cell of the human body contains 46 chromosomes. How many DNA molecules does this statement represent? How many different types of DNA molecules does it represent?arrow_forward
- You are going to participate in a debate on the issue of genetic engineering. One side must present legal, scientific, and moral issues for the limited use of this science; the other side will support unrestricted use of the technology.arrow_forwardEach of these six terms fits into one (and only one) of the following blanks: bases, proteins, amino acids, genes, chromosomes, codons. Write the letter corresponding to each space and then write which of the five terms goes with that letter. For instance, if you think the answer to space “a” is “chromosomes,” write a = chromosomes. DNA is found on pairs of ______a_______. ______b_______ are series of three _____c_____ that code for particular _____d_______. _____e_____ are specific strings of codons. Amino acids are put together to form ________f______.arrow_forwardWhat was the first, DNA or RNA? Justify your answer with solid reasonarrow_forward
- What results would you expect if the bacteriophage that Hershey and Chase used in their experiment had contained RNA instead of DNA?arrow_forwardRefer to Figure 2 and compare this with the DNA model in Figure 1. a. In what ways are they similar? b. In what ways are they different? c. What is the biological significance of such differences? Why is the DNA referred to as the genetic material?arrow_forwardIf the DNA of one human cell is stretched out, it would be almost 6 feet long and contain over three billion base pairs. How does all this fit into the nucleus of one cell?arrow_forward
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