Microbiology: An Evolving Science (Fourth Edition)
Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 7.3, Problem 1TQ
Summary Introduction

To review:

The semiconservative nature of deoxyribonucleic acid (DNA) replication using nitrogen isotopes based on their density gradient.

Introduction:

The replication of cellular DNA is semiconservative in which each daughter cell receives one parental and one newly synthesized strand. It involves the formation of a replication bubble with two replication forks in the bacterial cells. The replication is bidirectional as it begins at a fixed position and proceeds in the opposite direction.

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Explain the principles behind DNA isolation using silica membrane spin column from Qiagen. 2 After DNA isolation using Qiagen extraction kit, the final flow through was subjected to spectrophotometric measurements. Explain the situation if the absorbances were found to be: i) The absorbance at 260 nm is higher than absorbance at 280 nm? ii) The absorbance at 230 is higher than absorbance at 260 nm?
Why is the company Qiagen has more refined DNA extraction steps than a normal Strawberry DNA extraction practical? Summary of Qiagen DNA extraction steps Add ATL buffer and grind with sample. Add 20 microliters of enzyme Proteinase K to degrade protein into a 1.5-2ml microcentrifuge tube. Add 200 microlitres AL lysis buffer, and mix by vortexing for 5–10 seconds, which breaks cell membrane allowing DNA to be released. Incubate the sample at 56 degrees for 10 minutes.  Mix the cell lysate with 200 microlitres ethanol by pipetting it at the side of the microcentrifuge wall so DNA precipitates. The DNA forms a white layer and the remaining liquid is discarded. Pipet the mixture into DNeasy Mini spin column placed in a 2 ml collection tube. Centrifuge for a minute at 8000 rpm. Place the mini spin column into a 2 ml collection tube, add 500 µl Buffer AW1, and centrifuge for 1 min at 8000 rpm. Then add it to a new 2 ml collection tube (provided), add 500 µl Buffer AW1, and centrifuge for 1…
Agarose gel electrophoresis is a technique used to separate DNA fragments on an agarose matrix. The DNA is then viewed by staining or under UV light. (i) What is the direction of DNA migration in electric field on the agarose gel? · . (ii) Explain the principle for the migration of DNA molecules in an electric field. (iii) DNA loading dye is used to prepare DNA markers and samples for loading on the agarose gel. State the function of bromophenol blue in the loading dye.
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cell culture and growth media for Microbiology; Author: Scientist Cindy;https://www.youtube.com/watch?v=EjnQ3peWRek;License: Standard YouTube License, CC-BY