Microbiology: An Evolving Science (Fourth Edition)
Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 7, Problem 5RQ
Summary Introduction

To review:

DNA supercoiling and its importance for microbial genomes.

Introduction:

DNA (deoxyribonucleic acid) is the genetic material in majority of the organisms. It is composed of two strands made up of nucleotides which are coiled around each other. These strands unwind during the process of replication. The uncoiling is done by the virtue of certain special proteins.

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b) Describe how DNA is digested by different restriction enzymes c) Describe how gel electrophoresis is used to estimate the size of DNA fragments.
. Explain how gel electrophoresis is used to separate DNA fragments of different lengths.
Explain the difference between sequencing by chain termination and sequencing by synthesis. Which one does dideoxy sequencing use? Which one does next generation sequencing use?   Thank you
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