The transformation results below were obtained with 10 ul of intact plasmid DNA at nine concentrations. The following numbers of colonies are obtained when 100|ul of transformed cells are plated on selective medium: Fill in the following table: Concentration # colonies DNA mass of Fraction of Mass Transformation PGREEN (Concentration PGREEN in cell Cell efficiency suspension x volume OR X spread = 100 µl + total vol cell susp. (510 µl) HINT: this is constant Y÷ A suspension spread = x 10ul plasmid solution) (Colonies - Mass spread) C x Z = A See calculation Given= X Given=Y С. Z. 0.00001 ug/ul 4 0.00005 ug/ul | 12 0.0001 ug/ul 0.0005 ug/ul 32 125 0.001 ug/ul 442 0.005 ug/ul 542 0.01 ug/ul 0.05 ug/ul 507 475 0.1 ug/ul 516 0.5 µg/ul 505

Q: Kpnl 4. Plasmid Z has a size of 7 kb, and the map shows Kpnl (K) and Pstl (P) cut sites relative to…

A: Bg2(B) contians restriction site in between Pst1 and Kpn1. Given plasmid have one restriction site…

Q: The principle of the alkaline method for plasmid DNA isolation is: O A. An acidic solution…

A: Plasmid DNA isolation by alkaline lysis method This method is the most commonly used method to…

Q: You observe the following: Your Your friend's Gel Gel 120 kDa 100 kDa 120 kDa -100 kDa -80 kDa 80…

A: Blotting, as we all know, is a process that uses electrophoresis to separate DNA, RNA, and protein…

Q: A plasmid is cleaved by restriction endonucleases and analyzed by agarose gel electrophoresis.…

A: From the agarose gel image, we get information about the plasmid size and the restriction sites…

Q: A plasmid vector pBS281 is cleaved by the enzymeBamHI (5′ G^GATCC 3′), which recognizes only onesite…

A: Hi there! Since you have posted multiple questions we are answering only first two sub-parts.…

Q: The following plasmid is digested with EcoR1 and Ndel restriction endonucleases. Agarose…

A: Restriction endonucleases are enzymes which cleave the phosphodiester bonds within a DNA. These…

Q: This enzyme will be added when the cells are lysed, detroying all RNA in the sample. Why will this…

A: INTRODUCTION Plasmid Plasmid are circular DNA and into which we can insert the DNA of our interest…

Q: Please answer the following using the experiment data below. Provide the formulas or methods used…

A: Transformation efficiency is defined as the number of colonies obtained per microgram of DNA plated.

Q: This is a map of PBR322 plasmid Nid If a recombinant plasmid were obtained inserting DNA into the…

A: Ans: Recombinant DNA technology: The technique of cutting and pasting of DNA molecule with another…

Q: To verify the is indeed inside your plasmid, you'd like to do a colony PCR. But you need primers for…

A: introduction you can confirm the presence of gene within the vector by two ways either by…

Q: How many DNA bands would you observe in an ethidium bromide-stained electrophoresis gel if you…

A: In agarose gels and various cesium chloride gra- dient techniques, ethidium bromide is employed to…

Q: You are using these two plasmids to make a recombinant DNA molecule. You want to make a molecule,…

A: Recombinant technology includes the process in which we can introduce our gene of interest with a…

Q: Discuss the importance on the addition of ampicillin and arabinose to the medium of the genetically…

A: pGLO bacterial transformation experiment. Controls that were incubated with no plasmid (-pGLO) grow…

Q: 1. You have the plasmid pUC18/19, which is a circular plasmid that consists of 2686 bp. What would…

A: As per our guidelines, we are supposed to answer only one question. Kindly repost the other question…

Q: A plasmid DNA and a linear DNA (both of the same size) have one site for a restriction endonuclease.…

A: Because plasmid is a circular DNA molecule, this is the case. It becomes linear but not fragmented…

Q: Consider the following plasmid (size 8000 bp), with restriction sites at the positions indicated:…

A: A plasmid is determined as a very small and circular molecule of DNA, which is also double-stranded…

Q: These are the results after running Agarose Gel Electrophoresis of the cut/uncut pUC19 plasmid and…

A: These are the results after running Agarose Gel Electrophoresis of the cut/uncut pUC19 plasmid and…

Q: An E. coli Hfr (high frequency recombination) strain is defined as an E. coli strain that Select…

A: Bacteria reproduce via binary fission and splits into two identical daughter cells. Binary fission…

Q: You are about to isolate a 3000 bp large plasmid from an E.coli culture. You know that the plasmid…

A: 1bp dsDNA=650 Dalton 1 Dalton=1.66×10^(-24) grams ng is nanograms mcl is micro liters 1 gram=…

Q: Refer to the diagram of pUC18 (Fig.) to determine which restriction enzymes you could use to insert…

A: The PUC 18 is an artificial cloning vector, which is used in genetically engineered techniques. It…

Q: If the luciferase open reading frame is 1,650 bp in the control plasmid, and the Rel open reading…

A: The mRNA is produced from the DNA by transcription process and then this mRNA is used for the…

Q: plasmid has a total size of 2686 bp. There is an EcoRI recognition site at position 439, BamHI…

A: Plasmid  It is an extra chromosomal piece of DNA in the cell.

Q: Need help with question one

A: The plasmid is the extrachromosomal DNA present in the bacterial cell. These are the extra DNA other…

Q: Calculate the transformation efficiency of an experiment conducted using 10ul of 0.005 ug/ul…

A: Transformation is the process by which fragment of DNA is taken from the surrounding that causes…

Q: You transform 500 uL of cells with 2.5 ug of plasmid DNA, and then you take 100 uL and spread that…

A: Answer. Transformation efficiency Transformation efficiency is defined as the number of…

Q: It is desired to isolate genomic DNA from liquid culture of S. cerevisiae yeast. A commercial kit…

A: DNA is a double stranded helical molecule that is made up of repeating nucleotides. The nucleotides…

Q: You have another circular plasmid. Complete and effective digestion of this plasmid with a…

A: The restriction enzymes are endonuclease that means they can cut the DNA from the middle. They are…

Q: How do we explain the large expected difference in free energy of binding for high fidelity of DNA…

A: The accuracy of DNA replication is most critical to living organisms. The fidelity of replication is…

Q: For your 25ul ligation reaction: You need to add 160ng of your digested insert DNA (which is at a…

A: Given: Total volume - 25ul. The DNA need to be added is 160 ng. One ul contains 160 ng of digested…

Q: How many microliters of pGLO plasmid will you need for a PCR reaction is you need 200 nanograms and…

A: PCR (Polymerase Chain Reaction) is a series of three reactions that happen in order. It leads to the…

Q: You have isolated genomic DNA and plasmid DNA from a bacterial culture. Please answer the following…

A: Genomic DNA and plasmid DNA are isolated using silica resin columns than using traditional…

Q: You are about to isolate a 3000 bp large plasmid from an E.coli culture. You know that the plasmid…

A: The correct answer is E) 1.6 * 1012 cells

Q: You have just transformed 1 ul (100 pg/ul) of control pWasabi (plasmid) DNA into 50 ul of E. coli…

A:

Q: Restriction mapping of a plasmid: Digestion of a plasmid pCHEM1234 with the following restriction…

A: Here pCHEM1234 is digested with BamH1 and HindIII individually and in combination. Plasmid vector is…

Q: U have the plasmid pUC18/19, which is a circular plasmid that consists of 2686 bp. What would the…

A: A restriction endonuclease or restriction enzyme is a bacterial enzyme that cuts dsDNA into…

Q: You are about to isolate a 3000 bp large plasmid from an E.coli culture. You know that the plasmid…

A: Multiple questions asked. I will answer the first question as per guidelines. Please repost the…

Q: We make a 1:5 dilution of our 450 ng/ul PML104 plasmid, and then use 15 of the diluted sample in a…

A: Plasmid digestion refers to the digestion of plasmid using a restriction enzyme. Restriction enzymes…

Q: You are about to isolate a 3000 bp large plasmid from an E.coli culture. You know that the plasmid…

A:

Q: you have transformed E.coli cells with a plasmid containing the gene for the enzyme beta-lactamase…

A: Escherichia coli is a Gram Negative and facultative anaerobic bacteria. Competent cells are the…

Q: The gel profile has plasmid DNA digested by different restriction enzymes in lanes 1 to 4 (left to…

A: First option is incorrect because in the given diagram of plasmid DNA gel profile the DNAs in lanes…

Q: Using the formulae for dsDNA to calculate g/mol: You have a 4110 bp cloning vector Want to ligate a…

A: The average weight of a single DNA bp is 650 daltons. This can also be represented as 650 g/mol (=…

Q: For each of the following ( A & B ) provide the method of transfer and a brief explanation as to why…

A: Bacteria employs three ways for transferring the DNA :- 1. Conjugation  2. Transformation  3.…

Q: In a molecular biology laboratory, a student obtained competent E. coli cells and used a common…

A: Kanamycin is an aminoglycoside antibiotic.

Q: 1.1kbp 1.5 kbp Hindll Hindll 0.15 kbp amp 0.25 kbp If you were to digest this plasmid with HindllI,…

A: Plasmids are a small, extrachromosomal segment of DNA(deoxyribonucleic acid) and are mostly found in…

Q: 1. You have the plasmid pUC18/19, which is a circular plasmid that consists of 2686 bp. What would…

A: Hello. Since your question has multiple parts, we will solve the first question for you. If you want…

Q: DNA of different densities is distinguished by a procedure called caesium chloride (CsCI) density…

A: Cesium chloride (CsCI) density centrifugation generates a gradient of cesium and chloride ions.…

Q: Calculate how you would make 100 ul of a 2 pg/ul plasmid solution from a 5 ug/ul stock. If it is a 5…

A: The conversions required to solve this question are; 1 pg= 10-12g1 μl= 10-6l The product of Molarity…

Q: Define Buoyant density. How can you isolate genomic and plasmid DNA from E.coli using with this…

A: Buoyant density is defined as the density of a substance or molecule at which it attains equilibrium…

Bacterial Genomics

The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.

Transformation Experiment in Bacteria

In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.

Plasmids and Vectors

The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.