Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 7, Problem 10RQ
Summary Introduction
To review:
The solution for the problem of replication of both strands at a single replication fork.
Introduction:
The process of DNA (deoxyribonucleic acid) replication involves the synthesis of new DNA molecule by copying information from the template DNA strand in a semiconservative manner. DNA is a coiled structure and in order to get replicated, it must first unwind to expose the
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What is the end-replication problem? Why, in the absence of telomerase, do the ends of linear chromosomes get progressively shorter each time the DNA is replicated?
In terms of the new DNA strands that are generated, what are the differences between replication and conventional polymerase chain reaction?
How does the process of DNA replication generate mismatch mutations? What mechanisms are available to fix these errors during the process of replication?
Chapter 7 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 7.2 - Prob. 1TQCh. 7.2 - Prob. 2TQCh. 7.2 - Prob. 3TQCh. 7.2 - Prob. 4TQCh. 7.3 - Prob. 1TQCh. 7.3 - Prob. 2TQCh. 7.3 - Prob. 3TQCh. 7.3 - Prob. 4TQCh. 7.3 - Prob. 5TQCh. 7.6 - Prob. 1TQ
Ch. 7.6 - Prob. 2TQCh. 7.6 - Prob. 3TQCh. 7 - Prob. 1RQCh. 7 - Prob. 2RQCh. 7 - Prob. 3RQCh. 7 - Prob. 4RQCh. 7 - Prob. 5RQCh. 7 - Prob. 6RQCh. 7 - Prob. 7RQCh. 7 - Prob. 8RQCh. 7 - Prob. 9RQCh. 7 - Prob. 10RQCh. 7 - Prob. 11RQCh. 7 - Prob. 12RQCh. 7 - Prob. 13RQCh. 7 - Prob. 14RQCh. 7 - Prob. 15RQCh. 7 - Prob. 1TQCh. 7 - Prob. 2TQCh. 7 - Prob. 3TQ
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- How many replication forks are formed at the origin of replication?arrow_forwardIf the sequence 5′-AACGC-3′ were damaged by reactive oxygen species, what would be the most prevalent product, and what would be the result of replication? (Note: show both strands after replication)arrow_forwardHow fast does template DNA spin (expressed in revolutions per second) at an E. coli replication fork?arrow_forward
- What is the difference in DNA replicatoin on the leading strand versus the lagging strand?arrow_forwardWhat enzymatic features of DNA polymerase prevent it from replicating one of the DNA strands at the ends of linear chromosomes? Compared with DNA polymerase, how is telomerase different in its ability to synthesize a DNA strand? What does telomerase use as its template for the synthesis of a DNA strand? How does the use of this template result in a telomere sequence that is tandemly repetitive?arrow_forwardIn the Meselson-Stahl experiment on DNA replication, what fraction of the DNA was composed of one light strand and one heavy strand ("hybrid") after one generation of growth in medium containing 14N? After two generations of growth in a medium containing 14N? What fraction of hybrid DNA is expected after n generations of growth in a medium containing 14N?arrow_forward
- What is one enzyme that is involved with DNA replication and how would the absence of this enzyme affect replication of both prokaryotic and eukaryotic DNA?arrow_forwardIn terms of new DNA strands that are generated, what are the differences between replication and conventional polymerase chain reactions?arrow_forwardWhat is the difference between semi-conservative replication and dispersive replication?arrow_forward
- In eukaryotes, what is meant by the term DNA replication licensing? How does the process occur?arrow_forwardHow are deoxyribonucleoside triphosphates (precursors of daughter DNA strands) and ribonucleoside triphosphates (precursors of RNA strand) differ from each other?arrow_forwardConsidering that DNA is synthesized in the 5' to 3' direction, which direction must DNA polymerase travel along the template strand: 5' to 3' or 3' to 5' ?arrow_forward
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