Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 7.2, Problem 3TQ
Summary Introduction
To review:
The reason behind the visibility of nalidixic acid-resistant cells even after containing the mutation in gyrA.
Introduction:
The enzyme that changes the supercoiling of the deoxyribonucleic acid (DNA) or regulates the supercoiling level in DNA (deoxyribonucleic acid) is known as topoisomerase. There are two types of topoisomerase present in a cell, topoisomerase I and topoisomerase II. Type I topoisomerase enzymes function by creating transient a single-stranded breaks while type II introduces transient double-stranded breaks.
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Chapter 7 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 7.2 - Prob. 1TQCh. 7.2 - Prob. 2TQCh. 7.2 - Prob. 3TQCh. 7.2 - Prob. 4TQCh. 7.3 - Prob. 1TQCh. 7.3 - Prob. 2TQCh. 7.3 - Prob. 3TQCh. 7.3 - Prob. 4TQCh. 7.3 - Prob. 5TQCh. 7.6 - Prob. 1TQ
Ch. 7.6 - Prob. 2TQCh. 7.6 - Prob. 3TQCh. 7 - Prob. 1RQCh. 7 - Prob. 2RQCh. 7 - Prob. 3RQCh. 7 - Prob. 4RQCh. 7 - Prob. 5RQCh. 7 - Prob. 6RQCh. 7 - Prob. 7RQCh. 7 - Prob. 8RQCh. 7 - Prob. 9RQCh. 7 - Prob. 10RQCh. 7 - Prob. 11RQCh. 7 - Prob. 12RQCh. 7 - Prob. 13RQCh. 7 - Prob. 14RQCh. 7 - Prob. 15RQCh. 7 - Prob. 1TQCh. 7 - Prob. 2TQCh. 7 - Prob. 3TQ
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- In E. coli, all newly synthesized DNA appears to be fragmented (an observation that could be interpreted to mean that the leading strand as well as the lagging strand is synthesized discontinuously). However, in E. coli mutants that are defective in uracil–DNA glycosylase, only about half the newly synthesized DNA is fragmented. Explain.arrow_forwardCTP synthetase catalyzes the glutamine-dependent conversion of UTP to CTP. The enzyme is allosterically inhibited by the product, CTP. Mamma- lian cells defective in this allosteric inhibition are found to have a complex phenotype: They require thymidine in the growth medium, they have unbal- anced nucleotide pools, and they have an elevated spontaneous mutation rate. Explain the likely basis for these observations.arrow_forwardE. coli DNA polymerase V has the ability to bypass thymine dimers. However, Pol V tends to incorporate G rather than A opposite the damaged T bases. Would you expect Pol V to be more or less processive than Pol III? Explain.arrow_forward
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