Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 7, Problem 14EQ
In a cotransduction experiment involving P1, the cotransduction frequency was 0.53. How far apart are the two genes?
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Chapter 7 Solutions
Genetics: Analysis and Principles
Ch. 7.1 - 1. A form of genetic transfer that involves the...Ch. 7.2 - 1. A bacterial cell with an F factor conjugates...Ch. 7.2 - 2. Which of the following is a type of plasmid?...Ch. 7.3 - 1. With regard to conjugation, a key difference...Ch. 7.3 - 2. In mapping experiments, ______ strains are...Ch. 7.4 - Prob. 1COMQCh. 7.4 - Cotransduction may be used to map bacterial genes...Ch. 7.5 - Prob. 1COMQCh. 7.5 - Prob. 2COMQCh. 7.6 - 1. Which of the following is an example of...
Ch. 7 - 1. The terms conjugation, transduction, and...Ch. 7 - Prob. 2CONQCh. 7 - If you mix together an equal number of F+ and F...Ch. 7 - What is the difference between an F+ and an Hfr...Ch. 7 - 5. What is the role of the origin of transfer...Ch. 7 - 6. What is the role of sex pili during...Ch. 7 - Prob. 7CONQCh. 7 - Prob. 8CONQCh. 7 - Prob. 9CONQCh. 7 - 10. What is cotransduction? What determines the...Ch. 7 - Prob. 11CONQCh. 7 - Prob. 12CONQCh. 7 - Describe the steps that occur during bacterial...Ch. 7 - Prob. 14CONQCh. 7 - Prob. 15CONQCh. 7 - Antibiotics such as tetracycline, streptomycin,...Ch. 7 - Prob. 1EQCh. 7 - 2. In the experiment of Figure 7.1, Lederberg and...Ch. 7 - Explain how a U-tube apparatus can distinguish...Ch. 7 - Prob. 4EQCh. 7 - 5. In a conjugation experiment, what is meant by...Ch. 7 - In your laboratory, you have an F strain of E....Ch. 7 - 7. As mentioned in question 2 of More Genetic...Ch. 7 - An Hfr strain that is hisE+ and pheA+ was mixed...Ch. 7 - Acridine orange is a chemical that inhibits the...Ch. 7 - Prob. 10EQCh. 7 - Prob. 11EQCh. 7 - Lets suppose a new strain of P1 phage has been...Ch. 7 - If two bacterial genes are 0.6 minute apart on the...Ch. 7 - 14. In a cotransduction experiment involving P1,...Ch. 7 - Prob. 15EQCh. 7 - Prob. 16EQCh. 7 - 1. Discuss the advantages of the genetic analysis...Ch. 7 - Prob. 2QSDC
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- In a cotransformation experiment (see question 4 of More GeneticTIPS), DNA was isolated from a donor strain that was proA+ andstrC+ and sensitive to tetracycline. (The proA and strC genes conferthe ability to synthesize proline and confer streptomycin resistance,respectively.) A recipient strain is proA− and strC− and isresistant to tetracycline. After transformation, the bacteria werefirst streaked on a medium containing proline, streptomycin, andtetracycline. Colonies were then restreaked on a medium containingstreptomycin and tetracycline. (Note: Each type of medium hadcarbon and nitrogen sources for growth.) The following resultswere obtained:70 colonies grew on the medium containing proline, streptomycin,and tetracycline, but only 2 of these 70 colonies grew whenrestreaked on the medium containing streptomycin and tetracyclinebut lacking proline. If we assume the average size of the DNA fragments is 2 minutes,how far apart are these two genes?arrow_forwardThe following recombinants are recovered when conjugation occurs between an a*d*g+ donor and an adg recipient. at d+ g+ = 84% a d g+ = 6% at d g+ = 10% a dt g+ = less than 1% What is the map distance between the a and d genes? 10 map units 74 map units less than 1 map unit 84 map units 6 map unitsarrow_forwardAfter Griffith injected the mouse with a heat-killed S strain and the live R strain, blood from the mouse contained live S strain. True or False?arrow_forward
- In a transformation experiment, donor DNA was obtained from a prototroph bacterial strain (x* y z'), and the recipient was a triple auxotroph (x y z ). The following transformant classes were obtained: x* y z xy'z 160 130 190 170 x'y 3 xy x'y 5 What general conclusions can you draw about the linkage relationships among the three genes?arrow_forwardAn Hfr strain that is leuA+ and thiL+ was mixed with a strain thatis leuA− and thiL−. In the data points shown in the following graph,the conjugation was interrupted at different time points, and thepercentage of recombinants for each gene was determined bystreaking on a medium that lacked either leucine or thiamine.What is the map distance (in minutes) between these two genes?arrow_forwardConsider the following types of cells: F+, F-, Hfr, and F’ cells. Which of these four types of cells are capable of acting as a donor during conjugation? What genes does each cell that is capable of acting as a donor donate to the recipient cell?arrow_forward
- Several cell culture lines of epithelial cells, called “Cell Line A, B, or C,” are incubated with 104 infectious particles of influenza virus, and viral titers in the culture media are measured 2 days later. Looking at the results of this experiment, it is apparent that the three lines do not all show the same response to the virus. To investigate these differences, mixing experiments are performed, where cells from two different cell lines are mixed together at a 1:1 ratio before the Influenza infection. Based on the results shown in the figure below, propose an explanation for these data.arrow_forwardFour Hfr strains are derived from an F+ strain of E. Coli to serve as donors for an interrupted-mating experiment. Use the time-table and partial map of the F+ strain (shown below) to determine the genes’ respective positions. Keep in mind that the map distances are NOT proportional, only the FIRST 5 markers are indicated per strain, and the entry times, recorded in minutes, are in parentheses. Transferred genes represent wild-type alleles. Based on the data, which gene can be located at position 5 on the map?arrow_forwardA series of Hfr strains that have genotype m+ n+ o+ p+ q+ r+ are mixed with an F− strain that has genotype m− n− o− p− q− r−. Conjugation is interrupted at regular intervals and the order of the appearance of genes from the Hfr strain is determined in the recipient cells. The order of gene transfer for each Hfr strain is What is the order of genes on the circular bacterial chromosome? For each Hfr strain, give the location of the F factor in the chromosome and its polarity. Hfr5 m+ q+ p+ n+ r+ o+ Hfr4 n+ r+ o+ m+ q+ p+ Hfr1 o+ m+ q+ p+ n+ r+ Hfr9 q+ m+ o+ r+ n+ p+arrow_forward
- A fluctuation test was carried out to determine the rate of mutation to Azetidine resistance (a toxic proline analog) in S. typhimurium. Twenty tubes of rich medium were each inoculated with a few wild-type cells and the cultures were grown to 10° cells / ml. A 0.1 ml sample of each culture was then plated on each plate (a total of 20 plates) to detect AztR mutants. The results are shown in the following table. Calculate the mutation rate of S. typhimurium to AztR. Culture # # AztR mutants Culture # # AztR mutants 11 12 3 4. 13 14 15 4. 30 303 97 69 14 16 17 18 19 20 10 19arrow_forwardBacteriophage P22 was used in generalised transduction experiments to infect the Salmonella typhimurium donor strains described in the table below. The resulting phage lysates were then used to infect the recipient strains of S. typhimurium recipient strains listed in the table. In each cross, a phenotype was selected for one of the selected for one of the three genetic markers studied (str, aceA, thrA), and were made to select the recombinants corresponding to the other two markers. markers. The results are given in the following table: Strain I donor str thrA aceA thrA str aceA+ Strain recipient strs thrA+ aceA thrA str aceA Phenotype selected Str Ace+ Str recombinants selected ThrA ThrA ThrA ThrA Ace Ace Number 60 40 95 5 10 90 str: gene involved in streptomycin resistance, aceA: gene involved in the use of acetate as a carbon source, thrA: gene involved in threonine biosynthesis. 1) What are the selective media used in these three transduction experiments? to obtain the selected…arrow_forwardStreptococcus pneumoniae cells of genotype str s mtl - are transformed by donor DNA of genotype strr mtl+ and (in a separate experiment) by a mixture of two DNAs with genotypes strr mtl - and str s mtl+. The accompanying table shows the results.a. What does the first row of the table tell you? Why? b. What does the second row of the table tell you? Why?arrow_forward
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license