Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 7, Problem 4EQ
Summary Introduction
To review:
The interrupted mating experiment, the type of information obtained from its study and the reason behind the necessity to interrupt the mating.
Introduction:
Conjugation is the transmission of bacterial genetic matter via direct cellular contact or by the formation of a connection between two cells. This mechanism involves horizontal transfer of the genes. Other horizontal transfer methods include transduction and transformation.
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What is an interrupted mating experiment? What type of experimentalinformation can be obtained from this type of study? Why is it necessary to interrupt conjugation?
Sam wants to alter his interrupted mating experiment by placing a filter in between the donor and the recipient cells in order to separate the two. Is Sam’s suggestion for this modification to the procedure utilized in interrupted mating experiments valid? Explain.
a) Sam wants to alter his interrupted mating experiment by placing a filter in between the donor and the recipient cells in order to separate the two. Is Sam’s suggestion for this modification to the procedure utilized in interrupted mating experiments valid? Explain.
b) Much to the dismay of the researcher with whom Sam is working, Sam is not only forgetful, but he is also lazy! Rather than devoting the necessary hours to conducting his assigned interrupted mating experiments, Sam has decided that his time would be better spent modifying the F plasmid so that the experiments wouldn’t require quite so much time. The diagram below shows a map of the E. coli F plasmid, similar to that shown in lecture. Sam is determined to “get rid of some of the excess DNA” present in the plasmid. Please keep in mind that the PI in the lab in which Sam works utilizes these F plasmids for interrupted mating experiments between Hfr and F- cells as well as for interrupted mating experiments involving F+…
Chapter 7 Solutions
Genetics: Analysis and Principles
Ch. 7.1 - 1. A form of genetic transfer that involves the...Ch. 7.2 - 1. A bacterial cell with an F factor conjugates...Ch. 7.2 - 2. Which of the following is a type of plasmid?...Ch. 7.3 - 1. With regard to conjugation, a key difference...Ch. 7.3 - 2. In mapping experiments, ______ strains are...Ch. 7.4 - Prob. 1COMQCh. 7.4 - Cotransduction may be used to map bacterial genes...Ch. 7.5 - Prob. 1COMQCh. 7.5 - Prob. 2COMQCh. 7.6 - 1. Which of the following is an example of...
Ch. 7 - 1. The terms conjugation, transduction, and...Ch. 7 - Prob. 2CONQCh. 7 - If you mix together an equal number of F+ and F...Ch. 7 - What is the difference between an F+ and an Hfr...Ch. 7 - 5. What is the role of the origin of transfer...Ch. 7 - 6. What is the role of sex pili during...Ch. 7 - Prob. 7CONQCh. 7 - Prob. 8CONQCh. 7 - Prob. 9CONQCh. 7 - 10. What is cotransduction? What determines the...Ch. 7 - Prob. 11CONQCh. 7 - Prob. 12CONQCh. 7 - Describe the steps that occur during bacterial...Ch. 7 - Prob. 14CONQCh. 7 - Prob. 15CONQCh. 7 - Antibiotics such as tetracycline, streptomycin,...Ch. 7 - Prob. 1EQCh. 7 - 2. In the experiment of Figure 7.1, Lederberg and...Ch. 7 - Explain how a U-tube apparatus can distinguish...Ch. 7 - Prob. 4EQCh. 7 - 5. In a conjugation experiment, what is meant by...Ch. 7 - In your laboratory, you have an F strain of E....Ch. 7 - 7. As mentioned in question 2 of More Genetic...Ch. 7 - An Hfr strain that is hisE+ and pheA+ was mixed...Ch. 7 - Acridine orange is a chemical that inhibits the...Ch. 7 - Prob. 10EQCh. 7 - Prob. 11EQCh. 7 - Lets suppose a new strain of P1 phage has been...Ch. 7 - If two bacterial genes are 0.6 minute apart on the...Ch. 7 - 14. In a cotransduction experiment involving P1,...Ch. 7 - Prob. 15EQCh. 7 - Prob. 16EQCh. 7 - 1. Discuss the advantages of the genetic analysis...Ch. 7 - Prob. 2QSDC
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- The map below was constructed using information from an interrupted mating experiment. Str# refers to the strain numnber from which the data were obtained. The strains that are referenced by the Str#'s on the map are considered to be F+ strains. The units for the map distance values on this map are minutes Assume that four genes were transferred by each strain in the interrupted mating experiment. Based on this map, list in order of transfer (first to last) the genes transferred by Str4. Type your answer by typing in the letters that represent the genes without any spaces so that Moodle can grade it correctly. K Str2 10 9 Strl W B Str5 8. H. R Str3 Str6. 11 A Str4 12arrow_forwardHow do you use qPCR to confirm the phenotype of dpy-5 and skn-1. Describe the experiment.arrow_forwardWhat are noninformative matings. ?arrow_forward
- How can the concept ofrecombination frequency beused in genetic mapping?arrow_forwardHow can Cri-Du-Chat syndrome be diagnose with Fluorescence in situ hybridization(FISH)?arrow_forwardWith the technique of interrupted mating four Hfr strains were tested for the sequence in which they transmitted a number of different genes to an F strain. Each Hfr strain was found to transmit its genes is a unique sequence, as shown in the accompanying table. What is the gene sequence in the original strain from which these Hfr strains were derived?arrow_forward
- We used/chose a human Rab protein with no direct yeast equivalent as an “outgroup” for this study (image). Question: Why have we chosen a human Rab protein with no direct yeast equivalent as an “outgroup” for this study (image given)?arrow_forwardWhat is inverse PCR? How are we going to use inverse PCR to help figure out the molecular location of the EP element on Drosophila melanogaster flies?arrow_forwardWhat is your understanding of the Meselson & Stahl Experiment?arrow_forward
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