Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 7, Problem 5CONQ
What is the role of the origin of transfer during
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Wild-type bacteria were used as donors to transduce the
following recipient genotypes with the results indicated as
the number of wild type colonies:
Recipient
Recipient
leu- phe+ his- met+ tyr+
leu- phe+ his+ met- tyr+
leu- phe+ his+ met+ tyr-
leu- phe- his+ met+ tyr+
leu+ phe+ his-met- tyr+
127
leu+ phe-his- met+ tyr+
#wild type
37
138
132
1
leu+ phe+ his- met+ tyr-
leu+ phe-his+ met- tyr+
leu+ phe-hist met+ tyr-
leu- phe+ his+ met+ tyr+
22
6
leu+ phe+ his+ met- tyr-
148
31
304
leu+ phe+ his+ met+ tyr-
What is the order of the genes?
tyr-leu-his-phe-met
O leu-met-phe-his-tyr
leu-his-met-tyr-phe
his-tyr-leu-phe-met
#wild type
356
10
In E. coli, four Hfr strains donate the following markers,shown in the order donated:Strain 1: M Z X W CStrain 2: L A N C WStrain 3: A L B R UStrain 4: Z M U R BAll these Hfr strains are derived from the same F+ strain.What is the order of these markers on the circularchromosome of the original F+?
Given what we've discussed in class, what will be most likely outcome if
you conjugate an streptomycin resistant ampicillin sensitive methionine auxotroph E.
coli strain (engineered to be pir+) that is F- with a streptomycin sensitive non-HFR
methionine prototroph strain that is F- and RP4+ but contains pUC18?
Colonies on minimal media + ampicillin +streptomycin plates
No colonies on minimal media +ampicillin +streptomycin plates
Chapter 7 Solutions
Genetics: Analysis and Principles
Ch. 7.1 - 1. A form of genetic transfer that involves the...Ch. 7.2 - 1. A bacterial cell with an F factor conjugates...Ch. 7.2 - 2. Which of the following is a type of plasmid?...Ch. 7.3 - 1. With regard to conjugation, a key difference...Ch. 7.3 - 2. In mapping experiments, ______ strains are...Ch. 7.4 - Prob. 1COMQCh. 7.4 - Cotransduction may be used to map bacterial genes...Ch. 7.5 - Prob. 1COMQCh. 7.5 - Prob. 2COMQCh. 7.6 - 1. Which of the following is an example of...
Ch. 7 - 1. The terms conjugation, transduction, and...Ch. 7 - Prob. 2CONQCh. 7 - If you mix together an equal number of F+ and F...Ch. 7 - What is the difference between an F+ and an Hfr...Ch. 7 - 5. What is the role of the origin of transfer...Ch. 7 - 6. What is the role of sex pili during...Ch. 7 - Prob. 7CONQCh. 7 - Prob. 8CONQCh. 7 - Prob. 9CONQCh. 7 - 10. What is cotransduction? What determines the...Ch. 7 - Prob. 11CONQCh. 7 - Prob. 12CONQCh. 7 - Describe the steps that occur during bacterial...Ch. 7 - Prob. 14CONQCh. 7 - Prob. 15CONQCh. 7 - Antibiotics such as tetracycline, streptomycin,...Ch. 7 - Prob. 1EQCh. 7 - 2. In the experiment of Figure 7.1, Lederberg and...Ch. 7 - Explain how a U-tube apparatus can distinguish...Ch. 7 - Prob. 4EQCh. 7 - 5. In a conjugation experiment, what is meant by...Ch. 7 - In your laboratory, you have an F strain of E....Ch. 7 - 7. As mentioned in question 2 of More Genetic...Ch. 7 - An Hfr strain that is hisE+ and pheA+ was mixed...Ch. 7 - Acridine orange is a chemical that inhibits the...Ch. 7 - Prob. 10EQCh. 7 - Prob. 11EQCh. 7 - Lets suppose a new strain of P1 phage has been...Ch. 7 - If two bacterial genes are 0.6 minute apart on the...Ch. 7 - 14. In a cotransduction experiment involving P1,...Ch. 7 - Prob. 15EQCh. 7 - Prob. 16EQCh. 7 - 1. Discuss the advantages of the genetic analysis...Ch. 7 - Prob. 2QSDC
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- What are the functions of relaxase, coupling factor, and the exporter in the process of conjugation?arrow_forwardWhy are regulatory mutants used to increase the production of glutamic acid by Corynebacterium glutamicum?arrow_forwardA series of Hfr strains that have genotype m+ n+ o+ p+ q+ r+ are mixed with an F− strain that has genotype m− n− o− p− q− r−. Conjugation is interrupted at regular intervals and the order of the appearance of genes from the Hfr strain is determined in the recipient cells. The order of gene transfer for each Hfr strain is What is the order of genes on the circular bacterial chromosome? For each Hfr strain, give the location of the F factor in the chromosome and its polarity. Hfr5 m+ q+ p+ n+ r+ o+ Hfr4 n+ r+ o+ m+ q+ p+ Hfr1 o+ m+ q+ p+ n+ r+ Hfr9 q+ m+ o+ r+ n+ p+arrow_forward
- A pure culture of an unknown bacterium was streaked onto plates of a variety of media. You notice that the colony morphologyis strikingly different on plates of minimal media with glucose compared to that seen on trypticase soy agar plates. How can you explain these differences in colony morphology? Also, describe what happens when a nonsense mutation is introduced into the gene encoding transposase within a transposon and why is it more likely that insertions or deletions will be more detrimental to a cell than point mutations?arrow_forward"what is the reason for forming mucoid colonies in some types of bacteria?"arrow_forwardIn what ways does conjugation create bacterial cells that are at least partially diploid? Is this a stable condition?arrow_forward
- With respect to F+ and F- bacterial matings, answer the following questions: (a) How was it established that physical contact between cells was necessary? (b) How was it established that chromosome transfer was unidirectional? (c) What is the genetic basis for a bacterium’s being F+?arrow_forwardIn conjugation, how are donor and recipient cells brought intocontact with each other?arrow_forwardWhat is the role of sex pili during conjugation?arrow_forward
- The intermediates A, B, C, D, E, and F all occur in the same biochemical pathway G is the product of the pathway, and mutations 1 through 7 are all G –, meaning that they cannot produce substance G. The following table shows which intermediates will promote growth in each of the mutants. Arrange the intermediates in order of their occurrence in the pathway at which each mutant strain is blocked. A “+” in the table indicates that the strain will grow if given that substance, an “o” means lack of growth.arrow_forwardA shuttle vector is a vector constructed so that it can propagate in two different host species. One of the most common types of shuttle vectors is the yeast shuttle vector. (1) Name the THREE (3) most common yeast shuttle vectors. (ii) Based on the answer above, differentiate these THREE (3) vectors.arrow_forwardThree different Hfr donor strains are mixed with separate samples of an F− strain, and the following mapping data are provided from studies of interrupted conjugation: Appearance of genes in F − cells Hfr1: Genes b+ d+ c+ f+ g+ Time* 3 5 16 27 59 Hfr2: Genes e+ f+ c+ d+ b+ Time 6 24 35 46 48 Hfr3: Genes d+ c+ f+ e+ g+ Time 4 15 26 44 58 Construct a genetic map for these genes, indicating their order on the bacterial chromosome and the distances between them.arrow_forward
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