Genetics: Analysis and Principles
Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 7, Problem 7EQ

As mentioned in question 2 of More Genetic TIPS, origins of transfer can be located in many different locations, and their direction of transfer can be clockwise or counterclockwise. Let’s suppose a researcher conjugated six different Hfr strains that were t h r + l e u + t o n s s t r r a z i s l a c + g a l + p r o + m e t + to an F strain that was t h r l e u t o n r s t r s a z i r l a c g a l p r o m e t , and obtained the following results:

Strain Order of Gene Transfer
1 t o n s a z i s l e u + t h r + m e t + s t r r g a l + l a c + p r o +
2 l e u + a z i s t o n s p r o + l a c + g a l + s t r r m e t + t h r +
3 l a c + g a l + s t r r m e t + t h r + l e u + a z i s t o n s p r o +
4 l e u + t h r + m e t + s t r r g a l + l a c + p r o + t o n s a z i s
5 t o n s   p r o +   l a c +   g a l + s t r r m e t + t h r + l e u + a z i s
6 m e t + s t r r g a l + l a c + p r o + t o n s a z i s l e u + t h r +

Draw a circular map of the E. coli chromosome that shows the locations and orientations of the origins of transfer in these six Hfr strains.

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In E. coli, four Hfr strains donate the following markers,shown in the order donated:Strain 1: M Z X W CStrain 2: L A N C WStrain 3: A L B R UStrain 4: Z M U R BAll these Hfr strains are derived from the same F+ strain.What is the order of these markers on the circularchromosome of the original F+?
DNA from a strain of Bacillus subtilis with genotype a+ b+ c+ d+ e+ is used to transform a strain with genotype a− b− c− d− e−. Pairs of genes are checked for cotransformation, and the following results are obtained: Pair of genes Cotransformation Pair of genes Cotransformation a+ and b+ No b+ and d+ No a+ and c+ No b+ and e+ Yes a+ and d+ Yes c+ and d+ No a+ and e+ Yes c+ and e+ Yes b+ and c+ Yes d+ and e+ No On the basis of these results, what is the order of the genes on the bacterial chromosome?
As mentioned in question 2 of More Genetic TIPS, origins oftransfer can be located in many different places on a bacterial chromosome,and their direction of transfer can be clockwise or counterclockwise.Let’s suppose a researcher conjugated six differentHfr strains that were thr + leu + tons str r azis lac + gal + pro + met +to an F − strain that was thr − leu − tonr str s azir lac − gal − pro −met −, and obtained the following results: Draw a map of the circular E. coli chromosome that shows thelocations and orientations of the origins of transfer in these six Hfrstrains.

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Genetics: Analysis and Principles

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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license