Concept explainers
To review:
About transduction and how it takes place with the help of the lytic cycle. Also, the type ofgenetic material present in the P1 phage in the lysate and in the P1 phage.
Introduction:
Transduction occurs when the virus infects a host cell of bacteria and then transfers the genetic material into other bacterial cells through the infected bacteria. The bacteriophages have a protein layer around their genetic material and this helps them to bind with the bacterium. This occurs when the bacteriophage attaches itself to the bacteria. The introduction of genetic material of the virus-cell into the bacterial cell occurs through this process. The bacteriophage follows a lytic process and this helps them to inject the genetic material into the bacterial cells.
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Genetics: Analysis and Principles
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- The bacteriophage genome consists of many genes encoding proteins that make up the head, collar, tail, and tail fibers. When these genes are transcribed following phage infection, how are these proteins synthesized, since the phage genome lacks genes essential to ribosome structure?arrow_forwardUsually, bacteria only make tryptophan when tryptophan is absent or available in low concentration. However, a particular bacterial mutation makes tryptophan all the time whether or not tryptophan is present. What could explain this phenotype? A) the terminator hairpin is unable to form B) the antiterminator hairpin is unable to form C) trpE is mutated D) trpD is mutated E) trpA is mutatedarrow_forwardWhich of the following terms describes bacteriophage DNA that has become integrated into the host cell chromosome? A) plasmid B) impolite phage C) transposon D) prophagearrow_forward
- Scientists at the SEA Phages lab were asked to find a phage that might infect a strain of M. abscessus that is infecting a young patient. Because the scientists did not have any phages known to infect M. abscessus, they first tested M. smeg phages that are also known to infect M. tuberculosis to see if they could infect this specific strain of M. abscessus. Why did they start with those phages? Select the best answer. O Phages that infect M. tuberculosis will also infect M. abscessus O The phages have already been FDA approved for use in phage therapy O Phages that infect two different hosts have a broader host range and are more likely to be able to infect a third host (such as M. abcessus) O The phages are lytic, which makes them good candidates for phage therapy O More information is known about the genomes of these phages than other phagesarrow_forwardFrom the perspective of a bacteriophage, what is the advantage of being able to follow either a lytic or a lysogenic cycle?arrow_forwardWhat is the difference between the lytic and lysogenic cycle of bacteriophages? In the lysogenic cycle, the phage DNA integrates itself into the host genome after penetration, and continues to biosynthesis when conditions change The host cell is not killed in the lysogenic cycle The lytic cycle occurs in the nucleus, while the lysogenic cycle occurs in the cytoplasm In the lysogenic cycle, penetration is achieved through phagocytosisarrow_forward
- If we were to examine a strain with the F plasmid inserted into the same site of the bacterial chromosome, but in the reverse orientation: a) What would the order of gene transfer be? Include all of the genetic markers including the amino acid and nucleic acid metabolism genes and streptomycin resistance. b) What cell types would be able to grow on the NA vs ECM media types? Be sure to include the genotypes of the cells that would grow. Remember that NA provides all nutrients the bacteria needs + no antibiotic and HCM = minimal medium + glucose + has streptomycin antibiotic c) Would we still be able to perform our mapping? Why or why not? (Hint: refer to part b above)arrow_forwardThe linear dsDNA genome of λ binds on the LamB receptor of E. Coli and conducts a normal lysogenic cycle. Exposure to stress will cause the excision of λ prophage from the E. Coli genome. The excised λ genome is then replicated, packaged, and released from the cell as mature λ phage particles and ready to infect other bacterial cells. Among λ phage particles,the transducing phage mediates a specific type of recombination. Understand this scenario and answer the following questions. 1. What are the basic requirements for the insertion of λ into the E. Coli genome? 2. What special features are found in the λ insertion site? 3. What type of recombination occurs with λ insertion in the E. Coli genome? 4. How you will differentiate λ transducing phage from normal λ phage? 5. What exclusive mechanism λ phage utilizes for recombination?arrow_forwardThe size of a DNA fragment that can be inserted into an unmodified λ vector is very limited. Large segment in the central region of the λ DNA molecule can be removed without affecting the ability of the phage to infect Escherichia coli cells. However, the deletion of this non- essential region does affect the life cycle of the bacteriophage. Explain this statement.arrow_forward
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