Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 7, Problem 15CONQ
Summary Introduction
To review:
The molecular mechanism of transformation, and the process using which bacteria absorb extracellular DNA (deoxyribonucleic acid). Also, the advantages for bacteria to import extra-chromosomal DNA into its chromosomes.
Introduction:
Transformation is a process in which genetic material is released into the environment when the bacterial cell dies. This material can then enter any other bacterial cell present in the environment.
The bacterial cells that absorb this DNA from the environment are known as competent cells, and then this genetic material reaches the chromosomes. The proteins present in these cells help in absorbing the extra-chromosomal DNA.
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Describe the mechanisms of horizontal gene transfer in bacteria, describing the manner in which the DNA can be transferred from one cell to another.Choose a disease or an organism that has a well-documented mechanism of resistance (such as methicillin resistant Staphylococcus aureus - MRSA or tuberculosis) and identify the gene or genes that confer resistance and the method of DNA transfer thought to contribute to its spread. This may be from one organism to another or may simply be passed down from mother to daughter cell due to environmental pressure.
In transformation, a bacterial cell takes up DNA fragments from its surroundings. The figure shows the process of natural
transformation. Position the labels below with the correct images.
(A) Donor and recipient genomes
The donor strand is integrated
into the recipient
chromosome and the displaced
bacterial DNA is degraded.
A wild-type donor cell is in the
vicinity of a double auxotroph. If
the wild-type cell lyses, it
releases DNA into the
environment.
One cell is identical to the
original recipient, while the other
carries the wild-type genes.
A competent recipient cells a
receptor site on its surface.
One strand of donor DNA enters the
cell and pairs with a homologous
region of the bacterial chromosome
The remaining DNA strand is
degraded.
A donor DNA strand binds to the
receptor site.
Reset
trpC1
Wild-type donor cell
Mechanism of natural transformation
Donor DNA
hal
Receptor site-
trpc/his double auxotroph
Recipient cell
Competent cell recipient
-Bacterial chromosome
(had.bpC")
Ma…
What is horizontal gene transfer? What are the three mechanisms for this to occur in bacteria?
What are the components needed for the processes of transformation, conjugation, and transduction? How does each process occur? What genes are involved in each process?
How do generalized and specialized transduction differ? What is the end result of each?
What is recombination? What is the importance to bacteria & archaea?
What are the two types of recombination? What are the details of each type? What components are needed for each type?
Chapter 7 Solutions
Genetics: Analysis and Principles
Ch. 7.1 - 1. A form of genetic transfer that involves the...Ch. 7.2 - 1. A bacterial cell with an F factor conjugates...Ch. 7.2 - 2. Which of the following is a type of plasmid?...Ch. 7.3 - 1. With regard to conjugation, a key difference...Ch. 7.3 - 2. In mapping experiments, ______ strains are...Ch. 7.4 - Prob. 1COMQCh. 7.4 - Cotransduction may be used to map bacterial genes...Ch. 7.5 - Prob. 1COMQCh. 7.5 - Prob. 2COMQCh. 7.6 - 1. Which of the following is an example of...
Ch. 7 - 1. The terms conjugation, transduction, and...Ch. 7 - Prob. 2CONQCh. 7 - If you mix together an equal number of F+ and F...Ch. 7 - What is the difference between an F+ and an Hfr...Ch. 7 - 5. What is the role of the origin of transfer...Ch. 7 - 6. What is the role of sex pili during...Ch. 7 - Prob. 7CONQCh. 7 - Prob. 8CONQCh. 7 - Prob. 9CONQCh. 7 - 10. What is cotransduction? What determines the...Ch. 7 - Prob. 11CONQCh. 7 - Prob. 12CONQCh. 7 - Describe the steps that occur during bacterial...Ch. 7 - Prob. 14CONQCh. 7 - Prob. 15CONQCh. 7 - Antibiotics such as tetracycline, streptomycin,...Ch. 7 - Prob. 1EQCh. 7 - 2. In the experiment of Figure 7.1, Lederberg and...Ch. 7 - Explain how a U-tube apparatus can distinguish...Ch. 7 - Prob. 4EQCh. 7 - 5. In a conjugation experiment, what is meant by...Ch. 7 - In your laboratory, you have an F strain of E....Ch. 7 - 7. As mentioned in question 2 of More Genetic...Ch. 7 - An Hfr strain that is hisE+ and pheA+ was mixed...Ch. 7 - Acridine orange is a chemical that inhibits the...Ch. 7 - Prob. 10EQCh. 7 - Prob. 11EQCh. 7 - Lets suppose a new strain of P1 phage has been...Ch. 7 - If two bacterial genes are 0.6 minute apart on the...Ch. 7 - 14. In a cotransduction experiment involving P1,...Ch. 7 - Prob. 15EQCh. 7 - Prob. 16EQCh. 7 - 1. Discuss the advantages of the genetic analysis...Ch. 7 - Prob. 2QSDC
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- Genetic transfer via transformation can also be used to map genes along the bacterial chromosome. In this approach, fragments of chromosomal DNA are isolated from one bacterial strain and used to transform another strain. The experimenter examines the transformed bacteria to see if they have incorporated two or more different genes. For example, the DNA may be isolated from a donor E. coli bacterium that has functional copies of the araB and leuD genes. Let’s call these genes araB+ and leuD+ to indicate the genes are functional. These two genes are required for arabinose metabolismand leucine synthesis, respectively. To map the distance betweenthese two genes via transformation, a recipient bacterium is used that is araB− and leuD−. Following transformation, the recipient bacterium may become araB+ and leuD+. This phenomenon is calledcotransformation because two genes from the donor bacterium have been transferred to the recipient via transformation. In this type of experiment, the…arrow_forwardGenetic transfer via transformation can also be used to map genes along the bacterial chromosome. In this approach, fragments of chromosomal DNA are isolated from one bacterial strain and used to transform another strain. The experimenter examines the transformed bacteria to see if they have incorporated two or more different genes. For example, the DNA may be isolated from a donor E. coli bacterium that has functional copies of the araB and leuD genes. Let’s call these genes araB+ and leuD+ to indicate the genes are functional. These two genes are required for arabinose metabolismand leucine synthesis, respectively. To map the distance betweenthese two genes via transformation, a recipient bacterium is used that is araB− and leuD−. Following transformation, the recipient bacterium may become araB+ and leuD+. This phenomenon is calledcotransformation because two genes from the donor bacterium have been transferred to the recipient via transformation. In this type of experiment, the…arrow_forwardResearchers who study the molecular mechanism of transformationhave identified many proteins in bacteria that function in theuptake of DNA from the environment and its recombination intothe host cell’s chromosome. This means that bacteria have evolvedmolecular mechanisms for the purpose of transformation by extracellularDNA. What advantage(s) does a bacterium derive from importing DNA from the environment and/or incorporating it into its chromosome?arrow_forward
- Genetic transfer via transformation can also be used to map genes along the bacterial chromosome. In this approach, fragments of chromosomal DNA are isolated from one bacterial strain and used to transform another strain. The experimenter examines the transformed bacteria to see if they have incorporated two or more different genes. For example, the DNA may be isolated from a donor E. coli bacterium that has functional copies of the araB and leuD genes. Let’s call these genes araB+ and leuD+ to indicate the genes are functional. These two genes are required for arabinose metabolismand leucine synthesis, respectively. To map the distance betweenthese two genes via transformation, a recipient bacterium is used that is araB− and leuD−. Following transformation, the recipient bacterium may become araB+ and leuD+. This phenomenon is calledcotransformation because two genes from the donor bacterium have been transferred to the recipient via transformation. In this type of experiment, the…arrow_forwardA hypothetical gene for cephalosporin resistance is found to be carried by a transposon. Explain what a transposon is. Then explain how the cephalosporin resistance could be horizontally transferred between organisms by transformation, conjugation, and transduction. What steps/events would have to occur to allow the transposon to be transferred by each method. Also, explain how it could be transferred vertically between organisms.arrow_forwardWhen an E. coli donor cell duplicates a strand of plasmid DNA, and passes this DNA strand to a recipient E. coli cell, without the use of naked DNA in solution or of a viral vector, this is: an example of horizontal gene transfer by means of lysogenic bacteriophages an example of horizontal gene transfer by means of lytic bacteriophages an example of horizontal gene transfer by means of transformation an example of horizontal gene transfer by means of transduction an example of horizontal gene transfer by means of conjugationarrow_forward
- High Frequency Recombination results in which of the following? O 1) Plasmid DNA incorporated into the cell 2) Genomic DNA incorporated into the cell 3) Viral DNA incorporated into the cell 4) Plasmid and genomic DNA from the donor cell is incorporated into the recipient cell's genome 5) The movement of genes within the cell through homologous recombinationarrow_forwardDespite being asexual, bacteria can develop genetic variability during the process of conjugation. An exogenous single-stranded piece of linear DNA can be sent from a donor into a recipient where it reforms double-stranded DNA that lines up next to a plasmid or the recipient's main genome and undergoes crossing over events to incorporate new alleles, or in some cases, entirely new genes. Consider a situation where an exogenote, which contains a series of six mutant LOF alleles, recombines with the donor's endogenote, which contains all wild type alleles, and pick the correct statement. a Recombination will produce a donor bacterium that is fully functional. b Recombination containing an odd number of cross-overs will keep the donor bacterium alive, but it will no longer be able to produce certain gene products since it now contains one of more LOF alleles. c Recombination containing an even number of cross-overs will keep the donor bacterium alive, but it will…arrow_forwardFor bacteria living in a rapidly changing environment, would a population of bacteria capable of conjugation be more successful than one that could not undergo conjugation? Explain why. Include in your answer a description of the process of conjugation and its advantages over other mechanisms of DNA transfer between prokaryotic organisms.arrow_forward
- We have two specific strains of E. coli that have shown horizontal gene transfer (HGT) when mixed. To experimentally determine the method of HGT that is happening, the following conditions are set up in different tubes of culture media: A) Donor and recipient strain mixed together (control - no treatment). B) Donor and recipient strains mixed together, DNase added (can digest DNA in solution, not within cells).C) Special tube containing a membrane filter (with pores that allow DNA and viruses to pass through, but not bacterial cells) that separates two compartments. Donor strain is added on one side, the recipient strain on the other (they are separated by the filter).D) Donor and recipient strains mixed together, with chemical that inactivates viruses (chemical affects bacteriophages in solution so they are unable to attach to cells). The results: Tubes A, B, and D: HGT was observed. Tube C: HGT was NOT observed. Based on this, which type of HGT was occurring? Conjugation,…arrow_forwardIt is desired to isolate genomic DNA from liquid culture of S. cerevisiae yeast. A commercial kit will be used to isolate genomic DNA from this liquid culture. Answer the following questions to understand the strategy used by commercial kits for genomic DNA isolation. a) List all the steps from cell pellet preparation to DNA elution. b) With which feature can the membrane in the column that comes with the commercial kit bind DNA? c) Which component in the kit would you use to recover the DNA from the membrane of the column to which the DNA was attached?arrow_forwardFour different types of molecules, namely A, B, C and D were isolated from a primitive life form and studied to determine which of these would have the characteristics of an ancient genetic material. The molecules were incorporated into vectors, which are the phages whose genome were transformed into recombinant DNA. The phages were then made to infect the host bacteria. Transfection with A increased bacterial motility; B resulted in the formation of a pili allowing bacteria to adh to surfaces; causes the overproduction of a membrane protein while D resulted in increased metabolism of the bacterial food source, glucose. Which of the molecules is most likely a genetic material for this primitive life form? А. А В. В С. С D. D E. all of thesearrow_forward
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license