Q: The figure below shows a partial chromosome map of an E. coli Hfr strain. Each mark = 10 minutes…
A: Given: A partial chromosome map of an E. coli Hfr strain. Each mark = 10 minutes between…
Q: An Hfrstrain that is a * b*c* d* ef g h*is mated with an F strain that is a b cd e fgh .The mating…
A: The gene that is transferred in the least time (that is during first interruption) is the first gene…
Q: A cross was performed between a yeast strain that requires methionine and lysine for growth (met−…
A: A cross had been performed in between yeast strain requiring lysine as well as methionine for growth…
Q: When the interrupted mating technique was used withfive different strains of Hfr bacteria, the…
A: Bacteria and other prokaryotes do not have a nucleus. Their genome is located in the cytoplasm of…
Q: I need the answer as soon as possible
A: Conjugation is the process of transfer of F-plasmid between bacterial cells. The cells which possess…
Q: Why are the recombinants produced from an Hfr x F- crossrarely, if ever, F+?
A: There are many methods which the bacteria used for the transfer of genes from one organism to…
Q: Let’s suppose a new strain of P1 phage has been identified thatpackages larger pieces of the E. coli…
A: When two genes are present close to each other in a chromosome, the bacteriophages can package the…
Q: Crosses are carried out between a bacterial F- strain and three different Hfr strains. In these…
A: The interrupted mating experiment is given in the question. It is used to find the order of genes…
Q: After conjugation, the donor cell is now F+ and lac+ v and the recipient cell is F+ and lac+ now
A: Answer: CONJUGATION : It serves as the process in bacteria through which one bacteria transfers the…
Q: From the data given, which statement below most accurately describes something that has occurred in…
A: It is the movement of water molecules from a solution with a high concentration to a solution with a…
Q: In Figure 5-12,a. Which Hfr gene enters the recipient last? (Whichdiagram shows it actually…
A: Some bacteria will have F-plasmid integrated with the chromosomal DNA, they are known as…
Q: As much fun as lInterrupted mating experiments are, they don't always capture a truly accurate…
A: From the given table it is observable that for the growth of P13 it is necessary to have a media…
Q: The schematic diagram below shows the total genomic DNA isolation of a bacterial colony on a mutant…
A: Centrifugation is done to divide the parts of liquid. Here in this DNA isolation process, the…
Q: An Hfr strain that is lacZ and galE" was mated to a strain that is lacZ and galE. The mating was…
A: Introduction: The transfer of the genetic material from one bacterium to another via the conjugation…
Q: You need to determine where your gene of interest lies on the bacterial chromosome. In order to…
A: Bacteria strain with Leu-bio-pro- are mixed with wild type Hfr strain . Also , pro gene is…
Q: In a conjugation experiment, what is meant by the time of entry?
A: Conjugation is nothing more than the fusion of two compatible bacterial cells. Bringing together and…
Q: A yeast geneticist irradiates haploid cells of a strain thatis an adenine-requiring auxotrophic…
A: A mutant strain that isn't able to grow on a minimal medium and requires some specific supplements…
Q: An Hfr strain that is hisE+ and pheA+ was mixed with a strain thatis hisE− and pheA−. The…
A: Transfer of genes in prokaryotes occurs through horizontal transfer mechanisms such as transduction,…
Q: A donor strain of bacteria with genotype leu+ gal− pro+ is infected with phages. The phage lysate…
A: The transduction is the process of transfer of genes of a genetic material from bacteria to the…
Q: An F' merozygote is formed as a result of the following events: 1) the recipient cell receives…
A: The inaccurately exercised plasmid from the chromosome of Hfr and causes the part of chromosomes (F…
Q: Beadle and Tatum Experiment X rays Conidia from the cultures that fail to grow on minimal medium are…
A: Beadle and Tatum experiment... Beadle and Tatum started their experiment by creating mutants of the…
Q: Figure 3 16 Mean 14 level 12 of expres 10 sion strain 17 strain 22 17 (arbitr ary units) 31 28 11 21…
A: Recombinant DNA technology alters the genetic material by inserting parts of DNA from various…
Q: An Hfr strain that is hisE+ and pheA+ was mixed with a strain thatis hisE− and pheA−. The…
A: Transfer of genes in prokaryotes occurs through horizontal transfer mechanisms such as transduction,…
Q: In threonine selected marker number of co-transformation rate is Met 2%-Gly 60%- Trp 10%. In…
A: Introduction: When transformation of two or more genes occur together simultaneously then this…
Q: DNA from a strain of Bacillus subtilis with the genotype trp" tyr* was used to transform a recipient…
A: Transformation is dependent on the competence of the cells i.e., whether a cell is competent enough…
Q: In this experiment, _____ plates were used to differentiate between red and white diploid yeast…
A: Yeast cells are eukaryotic, single-celled microorganisms that belong to the fungus kingdom. They are…
Q: Suppose a researcher conducts matings between three Hfr bacterial strains and an F¯ strain. The…
A: The F-plasmid in E. coli can exist as an extrachromosomal genetic element or can be integrated…
Q: A line of mouse cells is grown for many generations in a medium with 15N. Cells in G1 are then…
A: Since we only answer up to 3 sub-parts, we’ll answer the first three. Please resubmit the question…
Q: In an interrupted mating experiment, crosses of three different Hfr strains to an F- strain are…
A: Given: In the mating experiment, crosses of three different Hfr strains to an F- strain are carried…
Q: When demonstrating horizontal gene transfer, why is it important to use strains that each require at…
A: Introduction Gene transfer is referred to the process in which the DNA (Deoxyribose Nucleic Acid)…
Q: An F* strain, marked at 10 loci, gives rise spontaneously to Hfr progeny whenever the F factor…
A: Hfr Strain : Hfr is stands for high frequency of recombination, it is described by Luca Cavalli…
Q: When you testcross the offspring of ccWW x CCww, you get the following results: CcWw = 135 Ccww =…
A: A test cross is a cross between an individual organism with a dominating phenotype but uncertain…
Q: A prototrophic strain (his" arg" lac') was used as a donor to transform an auxotrophic strain (his…
A: Minimal media is the one which contains only nutrients essential for the growth of bacteria. There…
Q: An Hfr strain is used to map three genes in an interrupted mating experiment. The cross is…
A: rifamycin is used to confirm transformed bacteria as the Hfr has rif+ gene
Q: Match the following Conjugation terms with the most appropriate description in the image: F-…
A: Introduction Conjugation Is The Transfer Of Genetic Material From One Bacterium To Another Through…
Q: Most yeast grow suspended in culture, but some yeast grow as a film on the top of a liquid culture.…
A: Yeasts are unicellular eukaryotic organisms that are classified under the kingdom fungi. Yeasts are…
Q: You have conducted a transposon mutagenesis experiment using the same PRL27 system that was used…
A: Transposon mutagenesis, is a biological process that allows genes to be transferred to a host…
Q: An interrupted mating study is carried out on Hfr strains 1, 2, and 3. After conjugation is…
A: Conjugation is that the method by that one microorganism transfers genetic material to a different…
Q: Three pairs of bacterial cells with the given genotypes undergo conjugation. Place match the…
A: F+ bacteria contain gene for the formation of sex pillus which is important for the transfer of…
Q: Why are the recombinants produced from an Hfr * F- cross rarely, if ever, F+?
A: An Hfr cell is a bacterium with a conjugative plasmid (such as the F-factor) incorporated into its…
Q: An interrupted mating study is carried out on Hfr strains 1, 2 and 3. After conjugation is…
A:
Q: Four E. coli strains of genotype a*b¯ are labeled 1, 2, 3, and 4. Four strains of genotype a¯b* are…
A: ANSWER;- HFR is the high-frequency recombination cell F+ is the donor cell whereas F- is the…
Q: Below is the mapping data from three different crosses of Hfr strains with separate samples of an F-…
A: Hfr is a bacterium that has a recombined plasmid incorporated into its chromosomal DNA. Conjugation…
Q: An Hfr strain that is leuA+ and thiL+ was mixed with a strain thatis leuA− and thiL−. In the data…
A: Horizontal gene transfer, also known as lateral gene transfer, is a process in which one bacterium…
Q: . a. You want to perform an interrupted-mating mappingwith an E. coli Hfr strain that is Pyr+, Met+,…
A: Interrupted mating is a technique by which bacterial genes are mapped. In this technique, bacterial…
Q: A particular Hfr strain normally transmits the pro+marker as the last one in conjugation. In a cross…
A: If the cell such as a Hfr cell loses any of the F-plasmid sequence, then it gets reverted to a…
Q: An F' cell with a functional copy of the threonine gene on the bacterial chromosome, but with a…
A: Introduction Conjugation is the transfer of genetic material from one bacterium to another through…
Q: Which of the following statements about conjugation is false? After a F- cell has conjugated…
A: Bacteria have the ability to respond to selective pressures and will start to adapt to new…
Q: The schematic diagram below shows the total genomic DNA isolation of a bacterial colony on a mutant…
A: Cell centrifugation Centrifugation is a widely used technique in molecular laboratories to separate…
Q: An Hfr strain that is leuA+ and thiL+ was mixed with a strain thatis leuA− and thiL−. In the data…
A: Auxotrophs are microbes designed to or naturally lack some enzymes to produce essential nutrients.…
Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
An Hfr strain that is leuA+ and thiL+ was mixed with a strain that
is leuA− and thiL−. In the data points shown in the following graph,
the conjugation was interrupted at different time points, and the
percentage of recombinants for each gene was determined by
streaking on a medium that lacked either leucine or thiamine.
What is the map distance (in minutes) between these two genes?
Step by step
Solved in 2 steps
- Eight mutant bacteriophage strains cannot lyse a certain type of bacteria that can be lysed by wild-type bacteriophages. The mutant strains were allowed to infect the bacteria in a complementation test. A "+" indicates that lysis occurred with coinfection. A "-" indicates that lysis did not occur. GKWTMAQC G- ++++- K W T M A Q C - + +++ - ++++ - + + - + - + - - + + + A cistron is defined by no complementation in the How many genes are controlling lysis in this bacteriophage? (Use a number not a word in the space) configuration.An ade+ arg+ cys+ his+ leu+ pro+ bacterial strain is knownto be lysogenic for a newly discovered phage, but the siteof the prophage is not known. The bacterial map isleucysarghisadeproThe lysogenic strain is used as a source of the phage, andthe phages are added to a bacterial strain of genotypeade- arg- cys- his- leu- pro-. After a short incubation,samples of these bacteria are plated on six differentmedia, with the supplementations indicated in thefollowing table. The table also shows whether colonieswere observed on the various media.PresenceMedium Ade Arg Cys His Leu Pro of colonies1 - + + + + + N2 + - + + + + N3 + + - + + + C4 + + + - + + N5 + + + + - + C6 + + + + + - NNutrient supplementation in medium(In this table, a plus sign indicates the presence of anutrient supplement, a minus sign indicates that asupplement is not present, N indicates no colonies, and Cindicates colonies present.)a. What genetic process is at work here?b. What is the approximate locus of the prophage?2 3 inte tion of Lormhda. ne att si ucated betwee de ne att sites and go ns one te n vacter aged ot utilize doot ractose ato la t don s gal* uc Integration to form prophage om SOward cor coct some cro on! ni imple ex 2 3 bio* gal* 14. The figure provided portrays the integration of Lambda phage into a host chromosome at the att site, located between the gal+ and bio+ genes. This prophage may disintegrate from the host carrying with it host genes, such as gal+ and/or bio+ and go on to transduce another host bacterium. How would one determine if a gal- host bacterium's phenotype was changed from gal- to gal+? To clarify, the minus version cannot utilize galactose as a carbon source for growth because it does not produce galactase, the enzyme that hydrolyzes galactose into monomeric sugars. There is a straight forward answer/solution to this – do not concoct some crazy solution! Think simple experiment.
- Nine rII− mutants of bacteriophage T4 were used inpairwise infections of E. coli K(λ) hosts. Six of themutations in these phages are point mutations; theother three are deletions. The ability of the doubly infected cells to produce progeny phages in large numbers is scored in the following chart.1 2 3 4 5 6 7 8 91 − − + + − − − + +2 − + + − − − + +3 − − + − + − −4 − + − + − −5 − − − + +6 − − − −7 − + +8 − −9 −The same nine mutants were then used in pairwise infections of E. coli B hosts. The production of progenyphages that can subsequently lyse E. coli K(λ) hosts isnow scored. In the table, 0 means the progeny do notproduce any plaques on E. coli K(λ) cells; − meansthat only a very few progeny phages produce plaques;and + means that many progeny produce plaques(more than 10 times as many as in the − cases).1 2 3 4 5 6 7 8 91 − + + + + − − + +2 − + + + + − + +3 0 − + 0 + + −4 − + − + + +5 − + − + +6 0 0 − +7 0 + +8 − +9 −a. Which of the mutants are the three deletions? Whatcriteria did…Please select all cellular processes that do NOT rely on RecA protein function as far as we considered them. bacteriophage lambda switch to lytic stage Lacl repression O LexA repression O natural transformation ODNA damage response O homologous recombination insertion of lambda phage into chromosomeArial BIUA 11 + .. | I 1 I 3 I 4 i.) Fill in the table for each of the E. coli: (0) = No Activity (+) = Basal Activity and (+++) = High Activity E. coli chromosome F' Plasmid B-gal activity? Permease activity? When When When When Glucose is Lactose is Glucose is Lactose is present present present present a.) I+ P+ O+ Z+ Y+ Inone +++ +++ b.) I^[S] P+ O+ Z+ Y+ none c.) I+ P+ O^[c] Z+ Y+ none d.) I+ P+ O- Z- Y+ none e.) I+ P+ O+ Z+ Y+ I^[S] P+ O+ Z+ Y+ f.) I^[S] P+ O+ Z- Y+ I+ P+ O^[c] Z+ Y- g.) I^[TB] P+ O+ Z+ Y 1+ P+ O^[c] Z- Y+ h.) I+ P+ O^[c] Z+ Y- I+ P+ O+ Z** Y+ i.) I^[TB] P+ O^[c] Z+ Y- 1+ P+ O+ Z- Y+ Z** is a polar mutation ii. ) If the lac operon in 'a' carried a mutation in the CAP binding site that rendered it nonfunctional, how would that affect the level of ß-galactosidase protein activity with and without lactose present, why? MacBook Air 000
- This question combines your knowledge of bacterial conjugationand the genetic regulation that directs thephage λ reproductive cycles. When researchers mix donor Hfr strains with recipient F– bacteria that are lysogenic for phage λ, the conjugated cells survive normally. However, if donor Hfr strains that are lysogenic for phage λ conjugate with recipient F– bacteria that do not contain any phage λ, the recipient cells often lyse, due to the induction of λ into the lytic cycle. Based on your knowledge of the regulation of the two reproductive cycles of phage λ, explain this experimental observation.Three pairs of bacterial cells with the given genotypes undergo conjugation. Place match the genotype of each cell after conjugation to its initial genotype. F+ x F Hfr F- F' F- Answer Bank F F F+ Hfr What is the role of the F-factor in conjugation? It contains genes necessary for replication of the donor's F plasmid. can occur. It allows auxotrophic bacterial cells to survive on minimal medium so that conjugation It contains genes that force recombination between the donor and recipient chromosomes. It contains genes necessary for the formation of the pilus. O It degrades the chromosome of the recipient cell after conjugation.41 The initiation complex for translation includes transfer RNA-MET. Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. TRUE FALSE
- 21:14 +4G KB/S LTED 41 96 09.00 Vo Microsoft Word - Assignment 02.docx Assignment 02 1. Groups of alleles associated with the lactose operon are as follows (in order of dominance for each allelic series): repressor, IS (superrepressor), I *(inducible), and I "(constitutive); operator, OC (constitutive, cis dominant) and 0+ (inducible, cis- dominant); structural, Z and Y. (a) Which of the following genotypes will produce B-galactosidase and B-galactoside permease if lactose is present: (1) 1*0* Z* Y*, (2) 1 *OCZ+ Y+, (3) OCZ+ Y+, (4) IS O+ Z+ Y+, and (5) 10+ Z+Y+? (b) Which of the above genotypes will produce ß-galactosidase and ß-galactoside permease if lactose is absent? Why? 2. By what mechanism does the presence of tryptophan in the medium in which E. coli cells are growing result in premature termination or attenuation of transcription of the trp operon? 3. Suppose that you used site-specific mutagenesis to modify the trpL sequence such that the two UGG Trp codons at positions…11:19 A for the virulence of a phage. A bacteriophage solution of a given concentration is inoculated with the bacterial colonies and the number of plaques on the bacterial plate is counted. The plaques are the proxy for the number of phages present in the solution as each plaque is likely to come from a single parent phage. 3. Why is it that only a lytic phage will yield accurate results from this type of experiment? Why can't you use a latent or filamentous phage? | B I U A. Yeah I'm Q w ERTYUIO P AS D F GHJK L Z X C V B N M 123 space return lill ןכΣ P UTF-8"Chapter 12 Solutions 202 x 02-xythos.content.blackboardcdn.com/5b19550bob538/10621560?X-Blackboard-Expiration3=D1649052000 DWorksheet.docx Edits will not be automatically saved. aved changes MOu a Garamond 14. B. 三: 三 山 8. When placed in each of the following, indicate if a red blood cell will 1) not change 2) hemolyze 3) crenate 5%(m/v) glucose solution 1%(m/v) glucose solution 0.5%(m/v) NaCl solution 2%(m/v) NaCl solution