Concepts of Genetics (12th Edition)
12th Edition
ISBN: 9780134604718
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino, Darrell Killian
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 6, Problem 9PDQ
In a transformation experiment, donor DNA was obtained from a prototroph bacterial strain (a+b+c+), and the recipient was a triple auxotroph (a−b−c−). What general conclusions can you draw about the linkage relationships among the three genes from the following transformant classes that were recovered?
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
In a transformation experiment, donor DNA was obtained from aprototroph bacterial strain (a+b+c+), and the recipient was a tripleauxotroph (a-b-c-). What general conclusions can you draw aboutthe linkage relationships among the three genes from the followingtransformant classes that were recovered? a+ b- c- 180 a- b+ c- 150 a+ b+ c- 210 a- b- c+ 179 a+ b- c+ 2 a- b+ c+ 1 a+ b+ c+ 3
After mutagenesis of wild type Vibrio fisheri, you isolate two different mutant strains (A and B) that, unlike the wild type cells, fail to luminesce when grown to high density in a flask with appropriate medium. Curiously, however, when you inoculate both mutant strains in the same flask, you observe that the mixed (A+B) culture begins to emit light after growing dense.
a) What gene/functions are likely affected in each of the two mutants?
b) How does this explain their phenotypes?
a)Dr. Thisisaneasyexam decides to amplify a gene from a plasmid using PCR. She starts out with 6.6 x 10-14g of a 10 kb template in a 100 µl reaction. Assuming that the molecular weight of the average base pair is 660 Daltons calculate the number of molecules of the template.
b)Given that the concentration of each primer (20 base pairs each) is 0.1µM in this same reaction volume (100 µl) calculate the number of molecules of the primer present
Chapter 6 Solutions
Concepts of Genetics (12th Edition)
Ch. 6 - When the interrupted mating technique was used...Ch. 6 - In a transformation experiment involving a...Ch. 6 - In complementation studies of the rII locus of...Ch. 6 - A 4-month-old infant had been running a moderate...Ch. 6 - Prob. 2CSCh. 6 - Prob. 3CSCh. 6 - Prob. 4CSCh. 6 - HOW DO WE KNOW? In this chapter, we have focused...Ch. 6 - Review the Chapter Concepts list on p. 123. Many...Ch. 6 - With respect to F+ and F bacterial matings, answer...
Ch. 6 - List all major differences between (a) the F+ F...Ch. 6 - Describe the basis for chromosome mapping in the...Ch. 6 - In general, when recombination experiments are...Ch. 6 - Why are the recombinants produced from an Hfr F...Ch. 6 - Describe the origin of F bacteria and merozygotes.Ch. 6 - In a transformation experiment, donor DNA was...Ch. 6 - Describe the role of heteroduplex formation during...Ch. 6 - Explain the observations that led Zinder and...Ch. 6 - Prob. 12PDQCh. 6 - Two theoretical genetic strains of a virus (abc...Ch. 6 - The bacteriophage genome consists of many genes...Ch. 6 - If a single bacteriophage infects one E. coli cell...Ch. 6 - A phage-infected bacterial culture was subjected...Ch. 6 - In recombination studies of the rII locus in phage...Ch. 6 - In an analysis of rII mutants, complementation...Ch. 6 - If further testing of the mutations in Problem 18...Ch. 6 - Using mutants 2 and 3 from Problem 19, following...Ch. 6 - During the analysis of seven rII mutations in...Ch. 6 - In studies of recombination between mutants 1 and...Ch. 6 - Prob. 23ESPCh. 6 - An Hfr strain is used to map three genes in an...Ch. 6 - A plaque assay is performed beginning with 1 mL of...Ch. 6 - In a cotransformation experiment, using various...Ch. 6 - For the experiment in Problem 26, another gene, g,...Ch. 6 - Bacterial conjugation, mediated mainly by...Ch. 6 - A study was conducted in an attempt to determine...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Consider the following experiment. First, large populations of two mutant strains of Escherichia coli are mixed, each requiring a different, single amino acid. After plating them onto a minimal medium, 45 colonies grew. Which of the following may explain this result? A) The colonies may be due to back mutation (reversion). B) The colonies may be due to recombination. C) Either A or B is possible. D) Neither A nor B is possible.arrow_forwardStreptococcus pneumoniae cells of genotype str s mtl - are transformed by donor DNA of genotype strr mtl+ and (in a separate experiment) by a mixture of two DNAs with genotypes strr mtl - and str s mtl+. The accompanying table shows the results.a. What does the first row of the table tell you? Why? b. What does the second row of the table tell you? Why?arrow_forwardGenomic DNA from a family where sickle-cell disease is known to be hereditary, is digested with the restriction enzyme MstII and run in a Southern Blot. The blot is hybridised with two different 0.6 kb probes, both probes (indicated in red in the diagram below) are specific for the β-globin gene (indicated as grey arrow on the diagram below). The normal wild-type βA allele contains an MstII restriction site indicated with the asterisk (*) in the diagram below; in the mutated sickle-cell βS allele this restriction site has been lost. What size bands would you expect to see on the Southern blots using probe 1 and probe 2 for an individual with sickle cell disease (have 2 βS alleles)? Probe 1 Probe 2 (a) 0.6kb 0.6kb and 1.2kb (b) 0.6kb and 1.8kb 0.6kb, 1.2kb and 1.8kb (c) 1.2kb 0.6kb (d) 1.8kb 1.8kb a. (a) b. (b) c. (c) d. (d)arrow_forward
- Let’s suppose you make a transposon library of the cellulose-secreting bacterium Komagataeibacter xylinus, with the goal of finding mutants that produce higher than normal amounts of cellulose, which would be useful industrially. However, despite your best efforts you are unable to isolate any transposon mutants that make more cellulose than the wild-type strain.Why might this have failed? List as many reasons as you can think of.arrow_forwardF ′strains in E. coli are derived from Hfr strains. In some cases, these F ′strains show a high rate of integration back into the bacterial chromosome of a second strain. Furthermore, the site of integration is often the site occupied by the sex factor in the original Hfr strain (before production of the F ′strains). Explain these results.arrow_forwardFour Hfr strains (A, B, C, D), were all derived from a F+ strain of E. coli. When each strain is used as a donor in an interrupted-conjugation experiment, the entry times of the first five markers are indicated in the table below (time of entry in minutes is indicated in brackets). Below is a partial map of the F+ strain (distances not proportional). Match the position of all genes with their correct position on this map. Strain A ala+ (5) ser+ (10) ade+ (15) his+ (25) val+ (30) 3 5 2 7 4 1 6 Strain B ade+ (12) his+ (22) val+ (27) pro+ (42) met+ (45) > sert ade+- 1. lac+ his+ ala+ met+ mal+ pro+ val+ Strain C pro+ (1) met+ (6) lac+ (11) mal+ (21) ala+ (31) 7. Strain D met+ (10) pro+ (15) val+ (30) his+ (35) ade+ (45) 2. 6. 3. 5. 4. 1. position #1 2. position #2 3. position #3 4. position #4 5. position #5 6. position #6 7. position #7arrow_forward
- From an Escherichia coli strain, five Hfr strains were isolated. The location and orientation of the transfer origin of each Hfr strain is shown in Figure 1. You want to use these five strains to map the locus responsible for thiamine synthesis, called thi. Each Hfr strain is sensitive to rifampicin (Rifs) and thi*. Conjugation experiments are performed between each of the Hfr strains and an F strain Rif Thi™. 0 T leu 10 20 T nadD pyrC trp 40 T his 60 70 2) The results are shown in the following table: Donor strain Hfr1 Hfr2 Hfr3 Hfr4 Hfr5 cysG 80 90 1) What is the selection medium used in these conjugation experiments metA Colonies Thi+ 1000 0 400 0 25 100 Hfr1 Hfr2 Figure 1: Chromosome map of Escherichia coli. Five Hfr strains (Hfr1 to Hfr5) were isolated and the location and orientation of the origin of transfer is shown by the arrows in each Hfr strain. Distances in minutes are shown. Leu: leucine biosynthesis; nadD: NAD biosynthesis; pyrC: pyrimidine biosynthesis; trp: tryptophan…arrow_forwardA 2.0kb bacterial plasmid ‘BS1030’ is digested with the restriction endonuclease Sau3A; the plasmid map is depicted in the diagram below and the Sau3A (S) restriction sites are indicated. Which of the following DNA fragments do you expect to see on an agarose gel when you run Sau3A-digested plasmid ‘BS1030’ DNA? a. 250 bp, 450 bp, 550 bp, 1.1 kb, 1.5 kb and 2.0 kb b. 2.0kb c. 250 bp, 400 bp, 450 bp, 500 bp and 550 bp d. 100 bp, 200 bp, 250 bp, 400 bp, 500 bp and 550 bparrow_forwardIn a cotransformation experiment (see question 4 of More GeneticTIPS), DNA was isolated from a donor strain that was proA+ andstrC+ and sensitive to tetracycline. (The proA and strC genes conferthe ability to synthesize proline and confer streptomycin resistance,respectively.) A recipient strain is proA− and strC− and isresistant to tetracycline. After transformation, the bacteria werefirst streaked on a medium containing proline, streptomycin, andtetracycline. Colonies were then restreaked on a medium containingstreptomycin and tetracycline. (Note: Each type of medium hadcarbon and nitrogen sources for growth.) The following resultswere obtained:70 colonies grew on the medium containing proline, streptomycin,and tetracycline, but only 2 of these 70 colonies grew whenrestreaked on the medium containing streptomycin and tetracyclinebut lacking proline. If we assume the average size of the DNA fragments is 2 minutes,how far apart are these two genes?arrow_forward
- A prototrophic strain (his" arg" lac') was used as a donor to transform an auxotrophic strain (his arg lac'). Initial transformants are isolated on minimal medium + histidine + arginine + lactose - glucose. i. What genotypes will grow on this medium? ii. These colonies are replicated to minimal medium + histidine, and 50% of the original colonies grow. What genotypes will grow on this medium? iii. The original colonies are also replicated to minimal medium + arginine, and 10% of the colonies grow. What genotypes will grow on this medium? iv. The original colonies are also replicated to minimal medium. No colonies grow. Based on this information, what genotypes will grow on minimal medium + histidine and on minimal medium + arginine? What is the relative gene order for his, arg, and lac? Which two genes are closer? Explain your answer.arrow_forwardBACTERIA STRAIN A IS AUXOTROPHIC FOR METHIONINE AND STRAIN B IS AUXOTROPHIC FOR LEUCINE. A. WILL THESE STRAIN GROW ON MINIMAL MEDIUM (A MEDIUM THAT DOES NOT CONTAIN EITHER METHIONINE OR LEUCINE)? B. WHAT TYPE OF GENETIC RECOMBINATION MUST OCCUR TO RECOVER A PROTOTROPH?arrow_forwardDuring your experiment you analysed only a few of the recombinant clones for the presence of the highly repeated Aluelements. If you wanted to screen for a single-copy gene, you would need to screen a much larger genomic library. Assuming, that you already know the amino acid sequence of unicorn (a species with a similar physiology to humans) insulin, how would you construct a probe which would enable you to use nucleic acid hybridisation to screen a unicorn genomic DNA library for the insulin gene? Hint: you have access to any molecular biology reagents and equipment you might need, such as vectors, enzymes, and DNA sequencers.arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSON
Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Anatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
Bacterial Genomics and Metagenomics; Author: Quadram Institute;https://www.youtube.com/watch?v=_6IdVTAFXoU;License: Standard youtube license