Concepts of Genetics (12th Edition)
12th Edition
ISBN: 9780134604718
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino, Darrell Killian
Publisher: PEARSON
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Chapter 6, Problem 15PDQ
If a single bacteriophage infects one E. coli cell present on a lawn of bacteria and, upon lysis, yields 200 viable viruses, how many phages will exist in a single plaque if three more lytic cycles occur?
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Students have asked these similar questions
Which of the following terms describes bacteriophage DNA that has become integrated into the host cell chromosome?
A) plasmid
B) impolite phage
C) transposon
D) prophage
If a single bacteriophage infects one E. coli cell present on a lawn of bacteria and, upon lysis, yields 185 viable viruses, how many phages will exist in a single plaque if 5 more lytic cycles occur?
Express your answer using three significant figures (example: 1.11×10^3).
Which of the statements about bacteriophage is FALSE:
a)
specialized transduction is a low frequency event
b)
may accidently package host DNA into a phage particle
c)
may perform specialized transduction
d)
only have a lytic life cycle
Chapter 6 Solutions
Concepts of Genetics (12th Edition)
Ch. 6 - When the interrupted mating technique was used...Ch. 6 - In a transformation experiment involving a...Ch. 6 - In complementation studies of the rII locus of...Ch. 6 - A 4-month-old infant had been running a moderate...Ch. 6 - Prob. 2CSCh. 6 - Prob. 3CSCh. 6 - Prob. 4CSCh. 6 - HOW DO WE KNOW? In this chapter, we have focused...Ch. 6 - Review the Chapter Concepts list on p. 123. Many...Ch. 6 - With respect to F+ and F bacterial matings, answer...
Ch. 6 - List all major differences between (a) the F+ F...Ch. 6 - Describe the basis for chromosome mapping in the...Ch. 6 - In general, when recombination experiments are...Ch. 6 - Why are the recombinants produced from an Hfr F...Ch. 6 - Describe the origin of F bacteria and merozygotes.Ch. 6 - In a transformation experiment, donor DNA was...Ch. 6 - Describe the role of heteroduplex formation during...Ch. 6 - Explain the observations that led Zinder and...Ch. 6 - Prob. 12PDQCh. 6 - Two theoretical genetic strains of a virus (abc...Ch. 6 - The bacteriophage genome consists of many genes...Ch. 6 - If a single bacteriophage infects one E. coli cell...Ch. 6 - A phage-infected bacterial culture was subjected...Ch. 6 - In recombination studies of the rII locus in phage...Ch. 6 - In an analysis of rII mutants, complementation...Ch. 6 - If further testing of the mutations in Problem 18...Ch. 6 - Using mutants 2 and 3 from Problem 19, following...Ch. 6 - During the analysis of seven rII mutations in...Ch. 6 - In studies of recombination between mutants 1 and...Ch. 6 - Prob. 23ESPCh. 6 - An Hfr strain is used to map three genes in an...Ch. 6 - A plaque assay is performed beginning with 1 mL of...Ch. 6 - In a cotransformation experiment, using various...Ch. 6 - For the experiment in Problem 26, another gene, g,...Ch. 6 - Bacterial conjugation, mediated mainly by...Ch. 6 - A study was conducted in an attempt to determine...
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- Why is a webbed plate capable of producing a higher lysate titer (pfu/mL) than a cleared plate? a.) A webbed plate has plaques that have gone through more rounds of replication because the number of host cells did not limit phage replication, creating exponentially more phage particles b.) A webbed plate has no more host available for replication, meaning the plate is full of exponentially higher amounts of phage c.) A cleared plate has less bacteria than a webbed plate, so there are more plaques present d.) A cleared plate contains more phage than a webbed plate because it started with a more concentrated phage samplearrow_forwardWhich of the statements is TRUE about conjugation? a) Plasmid DNA is transferred from a donor (F+) cell b) It may only be performed by competent cells c) DNA is always integrated into recipient chromosome d) It may be specialized or generalizedarrow_forwardWhat does a bacteriophage accomplish with lysogeny? O 1) it allows the bacteriophage to destroy the host cell's DNA. 2) it enables the bacteriophage to take over the cell. O 3) it speeds up the viral infection cycle. 4) it allows the bacteriophage to infect cells it would not normally infect. O 5) the genetic material of the bacteriophage can be passed on to future generations of infected cells.arrow_forward
- 1 mL of a bacteriophage suspension is mixed with 20 mL of a bacterial culture and 50% of the phages adsorb. We know that the bacteriophage suspension had a concentration of 1x1010 viruses per mL, and the bacterial culture had a concentration of 3x108 bacteria per mL. What fraction of the cells is multiple infected?arrow_forwardWhich process listed below allows genetic material to be transferred from a virus-like particle that lacks genes for its own replication? A) Conjugation of an F+ plasmid B) Gene transfer through a gene transfer agent C) Transduction by a dsDNA phage Mu D) Transformation of a linear piece of DNAarrow_forwardThere have been recent outbreaks of dog flu in the US. Why doesn't this virus infect humans? A) The virus can replicate in cells of all species but can only egress from dog cells. B) The genetic code of the virus is the same as that in dog cells but is different from other organisms. C) The virus can only attach to dog cells. D) The virus can enter cells of all species but can only replicate in dog cells.arrow_forward
- One mL of a bacteriophage suspension is mixed with 20 mL of a bacterialculture and 50% of the phages adsorb. We know that the bacteriophagesuspension had a concentration of 1x10^10 viruses per mL, and the bacterialculture had a concentration of 3x10^8 bacteria per mL. How many viruses are adsorbed per cell (multiplicity of infection)?arrow_forwardYou are studying your favorite (hypothetical) bacteriophage named M277. It infects E. coli and stays dormant within the bacterial cell. How does a phage become dormant within a cell? Generally, describe the steps of infection by phage in this scenarioarrow_forwardWhy are prokaryotes able to replicate so much more quickly than eukaryotes? (Hint: What cellular components must be copied before a cell splits in two?) Why is this difference in replication rate an important part of our vulnerability to bacterial pathogens?arrow_forward
- Scientists at the SEA Phages lab were asked to find a phage that might infect a strain of M. abscessus that is infecting a young patient. Because the scientists did not have any phages known to infect M. abscessus, they first tested M. smeg phages that are also known to infect M. tuberculosis to see if they could infect this specific strain of M. abscessus. Why did they start with those phages? Select the best answer. O Phages that infect M. tuberculosis will also infect M. abscessus O The phages have already been FDA approved for use in phage therapy O Phages that infect two different hosts have a broader host range and are more likely to be able to infect a third host (such as M. abcessus) O The phages are lytic, which makes them good candidates for phage therapy O More information is known about the genomes of these phages than other phagesarrow_forwardWhich of the following statements is not applicable to conjugation? A) OF factors are transferrable plasmids passed from one cell to another during conjugation. B) O During conjugation, rolling circle replication transfers an F factor from an F+ cell to an F- cell. ) O An F factor acquired during conjugation will automatically be integrated into the recipient's chromosome. D) O Conjugation requires physical contact between cells via the formation of a sex pilus. E) O An Hfr strain is capable of transferring a portion of the chromosome during conjugation.arrow_forwardWhat would happen to a virus that found its way to an environment filled only with cells that were outside of its host range? a) This virus would evolve until it was able to use the surrounding cells as hosts b) This virus would actively transport itself to another area in search of host cells C) This virus would replicate until it found cells that were within its host range d) Nothing; this virus would not be replicatedarrow_forward
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